Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A7 F1 I2 R1 | 766 | 64.2 | 3232166 | 90.9% | 2938038 | 104.5 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,090,046 | G→A | *17* (TAG→TAA) | hisL → | his operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,090,046 | 0 | G | A | 100.0% | 203.6 / NA | 64 | *17* (TAG→TAA) | hisL | his operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/36); total (28/36) | |||||||||||
TATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAG > NC_000913/2089915‑2090181 | tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa > 1:355176/1‑139 (MQ=255)tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa > 1:326005/1‑139 (MQ=255) taAAAAAGCCCATGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg < 2:1046585/139‑1 (MQ=255) aaaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGa > 1:1221627/1‑139 (MQ=255) aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt < 2:1221627/139‑1 (MQ=255) aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt < 2:49952/139‑1 (MQ=255) aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt < 2:1527753/139‑1 (MQ=255) aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt > 2:167946/1‑139 (MQ=255) cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt > 2:1324811/1‑139 (MQ=255) cTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa < 2:326005/139‑1 (MQ=255) ttCTAAGGTGAAAGTGTTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg < 1:1426371/139‑1 (MQ=255) ttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg < 1:167946/139‑1 (MQ=255) cTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGAc > 1:175818/1‑139 (MQ=255) tGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAg > 1:1212869/1‑139 (MQ=255) gTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa < 2:719796/122‑1 (MQ=255) gTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa > 1:719796/1‑122 (MQ=255) tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg < 1:1324811/139‑1 (MQ=255) aGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTcc < 1:1410502/134‑1 (MQ=255) aGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTcc > 2:1410502/1‑134 (MQ=255) aaaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCAt < 2:175818/139‑1 (MQ=255) aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg > 2:1406106/1‑139 (MQ=255) aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCGTCCAGTGGTGCATg < 1:1406106/139‑1 (MQ=255) aGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa < 2:1423373/139‑1 (MQ=255) aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt > 2:1592064/1‑95 (MQ=255) aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt < 1:1592064/95‑1 (MQ=255) ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga < 2:355176/139‑1 (MQ=255) gAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaaa < 2:754330/139‑1 (MQ=255) aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGcccc < 2:973554/139‑1 (MQ=255) aCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAGGAGAAAGccccc < 1:983844/139‑1 (MQ=255) aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgtg < 1:1469145/85‑1 (MQ=255) aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgtg > 2:1469145/1‑85 (MQ=255) aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg > 1:105809/1‑83 (MQ=255) aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg < 2:105809/83‑1 (MQ=255) agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa > 1:1415643/1‑93 (MQ=255) agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa < 2:1415643/93‑1 (MQ=255) agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGa > 1:1443746/1‑132 (MQ=255) agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGa < 2:1443746/132‑1 (MQ=255) gaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg > 1:1350137/1‑72 (MQ=255) gaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg < 2:1350137/72‑1 (MQ=255) cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt > 1:507678/1‑60 (MQ=255) cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt < 2:507678/60‑1 (MQ=255) cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg > 1:1480771/1‑61 (MQ=255) cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg < 2:1480771/61‑1 (MQ=255) cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt < 1:1513209/58‑1 (MQ=255) cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt > 2:1513209/1‑58 (MQ=255) cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg > 1:236828/1‑87 (MQ=255) cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg < 2:236828/87‑1 (MQ=255) cgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg < 2:1385052/85‑1 (MQ=255) cgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg > 1:1385052/1‑85 (MQ=255) cgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt > 2:325367/1‑139 (MQ=255) ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCTAg > 2:1427047/1‑83 (MQ=255) ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg < 1:1427047/83‑1 (MQ=255) tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg < 1:325367/139‑1 (MQ=255) catcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg < 1:386149/112‑1 (MQ=255) catcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg > 2:386149/1‑112 (MQ=255) tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGAt > 1:901501/1‑90 (MQ=255) tcatcCTGACTAATCTTTAAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGAt < 2:901501/90‑1 (MQ=255) cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa < 1:1546681/130‑1 (MQ=255) cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa > 2:1546681/1‑130 (MQ=255) aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg < 1:314609/108‑1 (MQ=255) aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg > 2:314609/1‑108 (MQ=255) aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa < 2:917745/127‑1 (MQ=255) aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa > 1:917745/1‑127 (MQ=255) cTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag < 2:1212869/139‑1 (MQ=255) | TATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAG > NC_000913/2089915‑2090181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |