Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,096,356	C→T	A171A (GCC→GCT)	hisF →	imidazole glycerol phosphate synthase, catalytic subunit with HisH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,096,356	0	C	T	100.0%	1022.3 / NA	307	A171A (GCC→GCT)	hisF	imidazole glycerol phosphate synthase, catalytic subunit with HisH
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (170/137); total (170/137)

Only 100 of 307 total aligned reads displayed.

TGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACT  >  NC_000913/2096220‑2096489

tgGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTgg                                                                                                                                     >  1:1606737/1‑139 (MQ=255)

  gTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGgag                                                                                                                                   <  2:1362209/139‑1 (MQ=255)

   tCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGgaga                                                                                                                                  >  1:208042/1‑139 (MQ=255)

     ggTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGaaa                                                                                                                                >  1:1049208/1‑139 (MQ=255)

         ttGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGt                                                                                                                            <  2:21847/139‑1 (MQ=255)

          tGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAATGCTCGACTGGGTACTGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTc                                                                                                                           <  2:1072000/139‑1 (MQ=255)

          tGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTc                                                                                                                           >  2:1551889/1‑139 (MQ=255)

            aTACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCt                                                                                                                         >  2:845258/1‑139 (MQ=255)

            aTACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCt                                                                                                                         <  1:192767/139‑1 (MQ=255)

                  ggTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAatat                                                                                                                   <  1:703214/139‑1 (MQ=255)

                   gTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatg                                                                                                                  <  1:1551889/139‑1 (MQ=255)

                   gTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTAGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatg                                                                                                                  >  2:281634/1‑139 (MQ=255)

                    tACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatga                                                                                                                 >  1:912637/1‑139 (MQ=255)

                      cgacgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatgatg                                                                                                               >  2:373344/1‑139 (MQ=255)

                      cgacgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatgatg                                                                                                               >  2:1457229/1‑139 (MQ=255)

                      cgacgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatgatg                                                                                                               >  1:233195/1‑139 (MQ=255)

                        acgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGaaa                                                                                                                                >  2:1612955/1‑120 (MQ=255)

                        acgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGaa                                                                                                             >  1:56320/1‑139 (MQ=255)

                         cgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTc                                                                                                                           <  1:882881/124‑1 (MQ=255)

                         cgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTc                                                                                                                           >  2:882881/1‑124 (MQ=255)

                         cgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGaa                                                                                                             <  1:1454384/138‑1 (MQ=255)

                         cgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAAt                                                                                                            >  1:1418886/1‑139 (MQ=255)

                          gCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATc                                                                                                           >  1:106895/1‑139 (MQ=255)

                              aaaCCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGa                                                                                                       >  1:149877/1‑139 (MQ=255)

                                  cGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGgc                                                                                                   <  2:119114/139‑1 (MQ=255)

                                            cATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGgag                                                                                                                                   <  1:918994/97‑1 (MQ=255)

                                              tgtgAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGt                                                                                       >  1:592563/1‑139 (MQ=255)

                                              tgtgAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGt                                                                                       >  1:1147434/1‑139 (MQ=255)

                                                 gAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGAGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTAc                                                                                    <  1:1034495/139‑1 (MQ=255)

                                                   aTCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGa                                                                                  >  1:1105286/1‑139 (MQ=255)

                                                   aTCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGa                                                                                  >  1:350433/1‑139 (MQ=255)

                                                   aTCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGa                                                                                  >  2:164569/1‑139 (MQ=255)

                                                        tataCCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCg                                                                             <  2:592563/139‑1 (MQ=255)

                                                          taCCGGCGATGAAAGCCGCACCCGCGCCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                           <  2:181108/139‑1 (MQ=255)

                                                            ccGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCCCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaaca                                                                         >  2:969091/1‑139 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGaaa                                                                                                                                <  2:24514/83‑1 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTc                                                                                                                           <  2:1581676/88‑1 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaacaa                                                                        >  1:176472/1‑139 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTAGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTGACGACCTCGaacaa                                                                        >  1:1015077/1‑139 (MQ=255)

                                                              ggCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaacaac                                                                       >  1:111292/1‑139 (MQ=255)

                                                               gCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACt                                                                      >  2:1448373/1‑139 (MQ=255)

                                                               gCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACt                                                                      >  1:968684/1‑139 (MQ=255)

                                                                cGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTAc                                                                                    >  2:720496/1‑124 (MQ=255)

                                                                 gATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGa                                                                    >  2:765230/1‑139 (MQ=255)

                                                                  aTGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGATGGCGTGCGTAACGGTTACGACCTCGAACAACTGaa                                                                   >  1:1000021/1‑139 (MQ=255)

                                                                  aTGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGgcgtgc                                                                                               <  1:62857/111‑1 (MQ=255)

                                                                  aTGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGgcgtgc                                                                                               >  2:62857/1‑111 (MQ=255)

                                                                       aGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTa                                                                                     >  2:545188/1‑116 (MQ=255)

                                                                         ccGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTa                                                                                            <  1:1051667/107‑1 (MQ=255)

                                                                         ccGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTa                                                                                            >  2:1051667/1‑107 (MQ=255)

                                                                           gCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCg                                                          <  1:133716/139‑1 (MQ=255)

                                                                            cACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCaa                                                                                                                      >  1:1177498/1‑78 (MQ=255)

                                                                                  cgTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                           >  1:696005/1‑115 (MQ=255)

                                                                                       cTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCa                                              >  1:18631/1‑139 (MQ=255)

                                                                                        tCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGCCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCAc                                             <  2:1003999/139‑1 (MQ=255)

                                                                                         cAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                           <  2:41263/108‑1 (MQ=255)

                                                                                         cAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                           >  1:41263/1‑108 (MQ=255)

                                                                                            tGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTa                                                                                     <  1:1258625/95‑1 (MQ=255)

                                                                                               gAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCt                                                                                                                         <  2:1373931/56‑1 (MQ=255)

                                                                                                  aCGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGa                                   >  1:268349/1‑139 (MQ=255)

                                                                                                    gCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTa                                                                                            >  1:1629286/1‑80 (MQ=255)

                                                                                                    gCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGAtt                                 <  2:11446/139‑1 (MQ=255)

                                                                                                     cTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTa                                                                                            >  2:793686/1‑79 (MQ=255)

                                                                                                     cTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTa                                                                                            <  1:793686/79‑1 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGGAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGc                               >  1:1572610/1‑139 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatga                                                                                                                 <  2:1290613/57‑1 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATatga                                                                                                                 >  1:1290613/1‑57 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTa                                                                                     <  1:1534860/85‑1 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCg                                                                             <  2:504590/93‑1 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                           <  2:123510/95‑1 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGc                               >  2:438141/1‑139 (MQ=255)

                                                                                                        gACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTa                                                                                     <  1:846186/83‑1 (MQ=255)

                                                                                                        gACTGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCt                             >  2:671437/1‑139 (MQ=255)

                                                                                                         aCTGGGTACAGGAAGTGCAAAAACGCGGTGCTGgag                                                                                                                                   <  1:674025/36‑1 (MQ=37)

                                                                                                           tGGGTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCg                          <  2:923298/139‑1 (MQ=255)

                                                                                                             ggTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGa                                                                                                       >  2:734852/1‑60 (MQ=255)

                                                                                                             ggTACAGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaaca                                                                         <  2:868273/90‑1 (MQ=255)

                                                                                                                  aGGAAGTGCAAAAACGCGGTGCTGGAGAAATCGTc                                                                                                                           >  1:1208239/1‑35 (MQ=255)

                                                                                                                   ggAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGcgcg                  <  1:379111/139‑1 (MQ=255)

                                                                                                                    gAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCa                                              <  1:1370372/110‑1 (MQ=255)

                                                                                                                    gAAGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGt                        >  2:914216/1‑132 (MQ=255)

                                                                                                                     aaGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCggg                <  2:199319/139‑1 (MQ=255)

                                                                                                                     aaGTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCggg                <  1:731890/139‑1 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGTAGAAATCGTCCTCAATATTATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGcc                                               >  1:143112/1‑106 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                           <  2:110919/78‑1 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGcc                                               <  2:143112/106‑1 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGcgcg                  >  2:821882/1‑135 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa              >  1:596868/1‑139 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa              >  1:325992/1‑139 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa              <  2:701953/139‑1 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa              >  2:83537/1‑139 (MQ=255)

                                                                                                                       gTGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa              <  1:753647/139‑1 (MQ=255)

                                                                                                                       atGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa              >  2:1034694/2‑139 (MQ=255)

                                                                                                                        tGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCggg                >  1:506721/1‑136 (MQ=255)

                                                                                                                        tGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCAc             >  2:690113/1‑139 (MQ=255)

                                                                                                                        tGCAAAAACGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCAGATTGCCTCCGGTGGCGCGGGCAc             >  1:925776/1‑139 (MQ=255)

                                                                                                                            aaaaCGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaaca                                                                         >  2:1532002/1‑75 (MQ=255)

                                                                                                                            aaaaCGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATg         >  1:444107/1‑139 (MQ=255)

                                                                                                                               aCGCGGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGaa      <  2:337786/139‑1 (MQ=255)

                                                                                                                                  cgGTGCTGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAacac   <  2:1404185/139‑1 (MQ=255)

TGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACT  >  NC_000913/2096220‑2096489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A