Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 715 38.4 1606192 96.6% 1551581 120.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,099,237 G→A A126V (GCG→GTG)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,099,2370GA100.0% 176.2 / NA 55A126V (GCG→GTG) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/29);  total (26/29)

CGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGG  >  NC_000913/2099108‑2099370
                                                                                                                                 |                                                                                                                                     
cGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTg                                                                                                                                    <  2:23529/133‑1 (MQ=255)
cGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTg                                                                                                                                    >  1:23529/1‑133 (MQ=255)
  ttCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTaaaa                                                                                                                            <  1:501874/139‑1 (MQ=255)
     tGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCa                                                                                                                         <  1:431480/139‑1 (MQ=255)
                 aCGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGtt                                                                                                             >  1:503415/1‑139 (MQ=255)
                       aCCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                       <  2:404338/139‑1 (MQ=255)
                       aCCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                       <  2:665780/139‑1 (MQ=255)
                          gATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACTGTTGATTtgatg                                                                                                    >  1:499096/1‑139 (MQ=255)
                            tGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                  <  2:309773/139‑1 (MQ=255)
                            tGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                  >  2:479418/1‑139 (MQ=255)
                              aCAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCTGTAAAACCAACGGGAACCGTTGATTTGATGACCa                                                                                                >  2:514387/1‑139 (MQ=255)
                               cAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCAt                                                                                               >  2:159917/1‑139 (MQ=255)
                                  tACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                            <  2:312317/139‑1 (MQ=255)
                                      tgacgtgtGGAGATTATCGTCAAGGGCTTTCCCATAACGGAGGAACTCCGCGTAGAATATAAGATTTTCGTTGCGATATTTCTTATGCATCACTGCGGTAATACCAACCGGCACGGTTCATTTGATGCCCATAACCGCa                                                                                        <  1:595142/132‑1 (MQ=255)
                                       gAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAAAATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                       >  1:233141/1‑139 (MQ=255)
                                            aTGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                  <  2:518058/139‑1 (MQ=255)
                                             tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCCGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGAATAAGGAt                                                                                 >  2:475065/1‑139 (MQ=255)
                                             tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAt                                                                                 >  2:265682/1‑139 (MQ=255)
                                              ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                                <  1:197883/139‑1 (MQ=255)
                                              ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                                >  2:526945/1‑139 (MQ=255)
                                               gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCAt                                                                                               <  1:356639/123‑1 (MQ=255)
                                               gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCAt                                                                                               >  2:356639/1‑123 (MQ=255)
                                                     aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCa                                                                         >  1:23939/1‑139 (MQ=255)
                                                     aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCa                                                                         >  2:266384/1‑139 (MQ=255)
                                                        gTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                      <  1:145639/139‑1 (MQ=255)
                                                            aGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGt                                                                  >  2:787472/1‑139 (MQ=255)
                                                             gggCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTc                                                                 <  2:291414/139‑1 (MQ=255)
                                                                cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                       >  2:171522/1‑98 (MQ=255)
                                                                cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                       <  1:171522/98‑1 (MQ=255)
                                                                cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                              >  2:34465/1‑139 (MQ=255)
                                                                      ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                        <  1:266384/139‑1 (MQ=255)
                                                                       cTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACt                                                       <  2:23939/139‑1 (MQ=255)
                                                                          aCGGAGAAATTCCGGGGAGAATCTAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTc                                                                 <  2:484193/126‑1 (MQ=255)
                                                                          aCGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCTTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTc                                                                 >  1:484193/1‑126 (MQ=255)
                                                                            ggAGAAATTCCGGGGAGAAAATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                  <  2:233141/139‑1 (MQ=255)
                                                                                aaaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                              <  1:736343/139‑1 (MQ=255)
                                                                                aaaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                              <  1:337583/139‑1 (MQ=255)
                                                                                 aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                             <  1:140836/139‑1 (MQ=255)
                                                                                    tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTgg                                          <  1:787472/139‑1 (MQ=255)
                                                                                        gggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGt                                      >  1:625600/1‑139 (MQ=255)
                                                                                                   atatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAGCGGGAACCGTTGATTTGTTGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaagaat                           <  1:514387/139‑1 (MQ=255)
                                                                                                       tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                       >  2:657792/1‑59 (MQ=255)
                                                                                                       tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                       <  1:657792/59‑1 (MQ=255)
                                                                                                       tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGt                       >  2:492726/1‑139 (MQ=255)
                                                                                                         tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                        <  1:550267/104‑1 (MQ=255)
                                                                                                         tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                        >  2:550267/1‑104 (MQ=255)
                                                                                                         tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                             >  1:778743/1‑115 (MQ=255)
                                                                                                         tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                             <  2:778743/115‑1 (MQ=255)
                                                                                                         tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                     >  2:578550/1‑139 (MQ=255)
                                                                                                         tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                     >  2:598519/1‑139 (MQ=255)
                                                                                                          cAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                              <  2:139201/113‑1 (MQ=255)
                                                                                                          cAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                              >  1:139201/1‑113 (MQ=255)
                                                                                                                   atTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATa           <  1:694227/139‑1 (MQ=255)
                                                                                                                     ttCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGt         <  1:265682/139‑1 (MQ=255)
                                                                                                                            gCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTgg  >  1:619910/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                     
CGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGG  >  NC_000913/2099108‑2099370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A