Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 765 39.6 1867486 94.4% 1762906 108.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,099,237 G→A A126V (GCG→GTG)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,099,2370GA100.0% 128.2 / NA 41A126V (GCG→GTG) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

CGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099115‑2099371
                                                                                                                          |                                                                                                                                       
cgcgTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAAc                                                                                                                        >  1:118181/1‑139 (MQ=255)
             cTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGttgat                                                                                                           <  2:435985/139‑1 (MQ=255)
                aCCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGttgatttg                                                                                                        >  2:45622/1‑139 (MQ=255)
                          aTACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              <  2:117293/139‑1 (MQ=255)
                             cGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCg                                                                                           <  2:42571/139‑1 (MQ=255)
                                      tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:637345/139‑1 (MQ=255)
                                      tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAt                                                                                  >  2:236112/1‑139 (MQ=255)
                                       ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                                 >  1:541819/1‑139 (MQ=255)
                                                    aaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    >  1:157716/1‑139 (MQ=255)
                                                       ggCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCt                                                                 >  1:713476/1‑139 (MQ=255)
                                                         cTTTACCCTCACGGAGAAATTCCGGGGAGAATCTAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    <  2:594027/134‑1 (MQ=255)
                                                         cTTTACCCTCACGGAGAAATTCCGGGGAGAATATATTATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                   <  2:511770/103‑1 (MQ=255)
                                                         cTTTACCCTCACGGAGAAATTCCGGGGAGAATATATTATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                   >  1:511770/1‑103 (MQ=255)
                                                         cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    >  1:594027/1‑134 (MQ=255)
                                                         cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                               >  2:467592/1‑139 (MQ=255)
                                                         cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                               >  2:679642/1‑139 (MQ=255)
                                                          tttACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTa                                                              >  1:502984/1‑139 (MQ=255)
                                                                   aCGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAt                                                     >  1:734015/1‑139 (MQ=255)
                                                                     ggAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                   <  1:679642/139‑1 (MQ=255)
                                                                      gagaAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCt                                                  <  2:546876/139‑1 (MQ=255)
                                                                         aaaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                               <  1:325398/139‑1 (MQ=255)
                                                                          aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  2:502984/139‑1 (MQ=255)
                                                                                           aatatTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                              <  2:118716/90‑1 (MQ=255)
                                                                                           aatatTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                              >  1:118716/1‑90 (MQ=255)
                                                                                            atatTTTCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            <  2:157716/139‑1 (MQ=255)
                                                                                                tttCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        >  2:825832/1‑91 (MQ=255)
                                                                                                tttCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        <  1:825832/91‑1 (MQ=255)
                                                                                                tttCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  2:4418/117‑1 (MQ=255)
                                                                                                tttCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              >  1:4418/1‑117 (MQ=255)
                                                                                                tttCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGt                        >  1:389068/1‑139 (MQ=255)
                                                                                                  tCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACggg                                                                                                                     >  2:339645/1‑44 (MQ=255)
                                                                                                  tCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACggg                                                                                                                     <  1:339645/44‑1 (MQ=255)
                                                                                                  tCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              >  1:728402/1‑115 (MQ=255)
                                                                                                  tCAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  2:728402/115‑1 (MQ=255)
                                                                                                   cAGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGtttt                     <  2:438492/139‑1 (MQ=255)
                                                                                                    aGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  1:695196/1‑76 (MQ=255)
                                                                                                    aGTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  2:695196/76‑1 (MQ=255)
                                                                                                     gTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         >  2:21092/1‑101 (MQ=255)
                                                                                                     gTGCGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:21092/101‑1 (MQ=255)
                                                                                                        cGATATTTCTTATGCATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGAt                <  1:767643/139‑1 (MQ=255)
                                                                                                                      cATCACTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGa  <  1:467592/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                                       
CGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGC‑GGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099115‑2099371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A