Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,099,237	G→A	A126V (GCG→GTG)	ugd ←	UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,099,237	0	G	A	100.0%	145.4 / NA	45	A126V (GCG→GTG)	ugd	UDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/23); total (22/23)

GCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTG  >  NC_000913/2099102‑2099369

gcGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTg                                                                                                                                   >  2:1067261/1‑139 (MQ=255)

                     gAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCg                                                                                                              <  1:1067261/139‑1 (MQ=255)

                          cTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgat                                                                                                         <  2:1513460/139‑1 (MQ=255)

                             aCCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                      <  1:763248/139‑1 (MQ=255)

                                        tACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                           >  1:1613105/1‑139 (MQ=255)

                                             tgaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                      <  2:334768/137‑1 (MQ=255)

                                              aaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTaaaa                                                                                                                           >  2:478577/1‑101 (MQ=255)

                                              aaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTaaaa                                                                                                                           <  1:478577/101‑1 (MQ=255)

                                                   tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATAACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                 >  1:1235962/1‑138 (MQ=255)

                                                   tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATAACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                 <  2:1235962/138‑1 (MQ=255)

                                                    ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                               >  1:1565442/1‑139 (MQ=255)

                                                       gaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGAATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                            <  1:1477608/139‑1 (MQ=255)

                                                                aaaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGtt                                                                                                            >  2:219273/1‑98 (MQ=255)

                                                                aaaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGtt                                                                                                            <  1:219273/98‑1 (MQ=255)

                                                                aaaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                           >  2:868661/1‑115 (MQ=255)

                                                                aaaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                           <  1:868661/115‑1 (MQ=255)

                                                                  aGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                     >  2:2132/1‑135 (MQ=255)

                                                                  aGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                     <  1:2132/135‑1 (MQ=255)

                                                                       tttACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTa                                                            >  1:544932/1‑139 (MQ=255)

                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                       <  1:35255/139‑1 (MQ=255)

                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                       <  2:1613105/139‑1 (MQ=255)

                                                                              tCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTc                                                                <  2:782976/128‑1 (MQ=255)

                                                                              tCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTc                                                                >  1:782976/1‑128 (MQ=255)

                                                                                       aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                            <  2:1565442/139‑1 (MQ=255)

                                                                                            cGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGAt                                       >  1:1020037/1‑139 (MQ=255)

                                                                                                       taatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                               <  2:655640/88‑1 (MQ=255)

                                                                                                       taatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                               >  1:655640/1‑88 (MQ=255)

                                                                                                        aatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                       <  2:396294/111‑1 (MQ=255)

                                                                                                        aatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                       >  1:396294/1‑111 (MQ=255)

                                                                                                        aatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataa                           <  1:210480/139‑1 (MQ=255)

                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                     <  2:221228/76‑1 (MQ=255)

                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                     >  1:221228/1‑76 (MQ=255)

                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGt                      >  2:1534120/1‑139 (MQ=255)

                                                                                                              ttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGtt                     >  1:1438862/1‑139 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                       >  1:187936/1‑104 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                       <  2:187936/104‑1 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAtt                                                  <  2:1233849/109‑1 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAtt                                                  >  1:1233849/1‑109 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                             >  2:1029698/1‑114 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                             <  1:1029698/114‑1 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                          >  2:42535/1‑133 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                          <  1:42535/133‑1 (MQ=255)

                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                    >  1:65626/1‑139 (MQ=255)

                                                                                                                               tATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGt    <  2:1020037/139‑1 (MQ=255)

                                                                                                                                 tGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTg  >  1:250444/1‑139 (MQ=255)

GCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTG  >  NC_000913/2099102‑2099369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A