Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 760 67.6 3323148 92.7% 3080558 109.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,099,237 G→A A126V (GCG→GTG)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,099,2370GA100.0% 1185.8 / NA 353A126V (GCG→GTG) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (171/182);  total (171/182)

Only 100 of 353 total aligned reads displayed.

GCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099102‑2099371
                                                                                                                                       |                                                                                                                                      
 cGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGc                                                                                                                                    >  2:1039505/1‑139 (MQ=255)
    aaCGTTCTGCGCGTTCTGAACGCTAACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGt                                                                                                                                 <  2:1142589/139‑1 (MQ=255)
        ttCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTaaaa                                                                                                                             <  2:262982/139‑1 (MQ=255)
                  tCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGaa                                                                                                                   >  2:739291/1‑139 (MQ=255)
                           tCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttg                                                                                                             >  1:1592828/1‑136 (MQ=255)
                             aCCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  2:1431814/139‑1 (MQ=255)
                                gATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAAcc                                                                                                                           <  1:181500/117‑1 (MQ=255)
                                  tGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                   >  2:924277/1‑139 (MQ=255)
                                     cAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCAt                                                                                                >  1:195382/1‑139 (MQ=255)
                                       aTACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              <  1:97938/139‑1 (MQ=255)
                                       aTACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATAT‑ATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                             >  2:540008/1‑139 (MQ=255)
                                        tACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                             <  1:128829/139‑1 (MQ=255)
                                             gAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        <  2:874566/139‑1 (MQ=255)
                                             gAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        <  1:1375338/139‑1 (MQ=255)
                                              aaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTaaaa                                                                                                                             >  2:616109/1‑101 (MQ=255)
                                                  aTGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCGACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  2:964918/1‑139 (MQ=255)
                                                  aTGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  1:682314/1‑139 (MQ=255)
                                                  aTGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:603582/139‑1 (MQ=255)
                                                   tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAt                                                                                  <  2:1648301/139‑1 (MQ=255)
                                                     gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttga                                                                                                            >  2:16758/1‑111 (MQ=255)
                                                     gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              >  2:1396951/1‑125 (MQ=255)
                                                           aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        >  2:518727/1‑109 (MQ=255)
                                                           aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  1:518727/109‑1 (MQ=255)
                                                           aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCa                                                                          >  1:1450202/1‑139 (MQ=255)
                                                           aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCa                                                                          >  2:1272829/1‑139 (MQ=255)
                                                           aTCGTAAAGGGCTTTACCCCCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCa                                                                          >  2:215436/1‑139 (MQ=255)
                                                            tCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGGTATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAtc                                                                             <  2:1588780/135‑1 (MQ=255)
                                                             cGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGTGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        <  1:1039505/139‑1 (MQ=255)
                                                             cGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAttt                                                                                >  1:278183/1‑131 (MQ=255)
                                                              gTAAAGGGCTTTACCCCCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                       <  1:215436/139‑1 (MQ=255)
                                                               tAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              <  2:447163/115‑1 (MQ=255)
                                                               tAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              >  1:447163/1‑115 (MQ=255)
                                                               tAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTa                                                                      >  1:114816/1‑139 (MQ=255)
                                                                 aaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    <  1:245110/139‑1 (MQ=255)
                                                                  aGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGt                                                                   >  2:799895/1‑139 (MQ=255)
                                                                      cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAACCCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                               >  2:1186098/1‑139 (MQ=255)
                                                                      cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGtt                                                                                                              >  1:410486/1‑92 (MQ=255)
                                                                      cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGtt                                                                                                              <  1:1574440/92‑1 (MQ=255)
                                                                         tACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAAt                                                            >  1:141403/1‑139 (MQ=255)
                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  2:195382/139‑1 (MQ=255)
                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:330775/139‑1 (MQ=255)
                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  2:1568470/139‑1 (MQ=255)
                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:721892/139‑1 (MQ=255)
                                                                            ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTGTAATTAc                                                         <  2:1085495/139‑1 (MQ=255)
                                                                              tCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAAc                                                                                                                        <  2:885569/74‑1 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAGCCGTTGATTTGATGACCATa                                                                                               >  1:1090226/1‑98 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAcc                                                                                            <  1:1212661/101‑1 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  1:565638/1‑110 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  2:565638/110‑1 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCaag                                                                        >  1:1113809/1‑120 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                       <  1:804743/122‑1 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGa                                                      >  2:110914/1‑139 (MQ=255)
                                                                               cACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGa                                                      >  1:919581/1‑139 (MQ=255)
                                                                                aCGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAt                                                     >  1:1331649/1‑139 (MQ=255)
                                                                                      atatcgcactgaaaatataatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                               >  1:118570/14‑139 (MQ=255)
                                                                                      aaaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                       >  1:1624522/1‑115 (MQ=255)
                                                                                       aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  1:1230850/139‑1 (MQ=255)
                                                                                        aTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    <  2:425693/116‑1 (MQ=255)
                                                                                          tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTgg                                           <  1:1129198/139‑1 (MQ=255)
                                                                                             ggggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATg                                        <  1:835777/139‑1 (MQ=255)
                                                                                              gggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCAt                                                                                                <  2:159515/82‑1 (MQ=255)
                                                                                              gggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                       >  2:1240785/1‑91 (MQ=255)
                                                                                                  gaATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              <  2:385758/80‑1 (MQ=255)
                                                                                                   aatataatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:35413/116‑1 (MQ=255)
                                                                                                     tataatatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAATCTACACTGGATGTATTGaaa                                <  1:739291/139‑1 (MQ=255)
                                                                                                         atatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            <  2:1331649/139‑1 (MQ=255)
                                                                                                          tatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            >  2:249381/1‑138 (MQ=255)
                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        <  1:641831/75‑1 (MQ=255)
                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        >  2:641831/1‑75 (MQ=255)
                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  2:690721/106‑1 (MQ=255)
                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAtt                                                    >  1:880691/1‑111 (MQ=255)
                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            >  2:112082/1‑135 (MQ=255)
                                                                                                             tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGt                        >  2:1150729/1‑139 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGTATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        <  1:1169712/73‑1 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGTATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        >  2:1169712/1‑73 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  2:849204/78‑1 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTa                                                                      <  1:872146/91‑1 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    >  1:1648171/1‑93 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         >  1:852678/1‑104 (MQ=255)
                                                                                                               tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTa                          <  1:542987/135‑1 (MQ=255)
                                                                                                                 aGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  2:3325/55‑1 (MQ=255)
                                                                                                                 aGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTa                    >  1:464525/1‑139 (MQ=255)
                                                                                                                  gTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  2:1300865/101‑1 (MQ=255)
                                                                                                                  gTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         >  1:1300865/1‑101 (MQ=255)
                                                                                                                  gTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            >  2:1367340/1‑130 (MQ=255)
                                                                                                                  gTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAg                   >  1:1007545/1‑139 (MQ=255)
                                                                                                                     cGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGAt                >  2:1383972/1‑139 (MQ=255)
                                                                                                                         atTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATa            <  1:1383972/139‑1 (MQ=255)
                                                                                                                         atTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATa            <  1:1579668/139‑1 (MQ=255)
                                                                                                                           ttCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTc                                                                  >  2:1024087/1‑83 (MQ=255)
                                                                                                                             cTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:1493162/64‑1 (MQ=255)
                                                                                                                             cTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  2:1493162/1‑64 (MQ=255)
                                                                                                                              ttATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCgg       <  1:952793/139‑1 (MQ=255)
                                                                                                                               tATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttga                                                                                                       <  2:292580/42‑1 (MQ=255)
                                                                                                                               tATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATaa                                                                                      >  2:1474360/1‑59 (MQ=255)
                                                                                                                                aTGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGtt     >  1:134966/1‑139 (MQ=255)
                                                                                                                                 tGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTac                                                <  1:824361/95‑1 (MQ=255)
                                                                                                                                 tGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTac                                                >  2:824361/1‑95 (MQ=255)
                                                                                                                                    aTCACTGCGGTAAAACCAACGGGAACCGttgatttgat                                                                                                      >  2:1209122/1‑38 (MQ=255)
                                                                                                                                    aTCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  2:947561/94‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099102‑2099371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A