Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,099,237 G→A A126V (GCG→GTG)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,099,2370GA100.0% 215.0 / NA 66A126V (GCG→GTG) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/35);  total (31/35)

AGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099101‑2099371
                                                                                                                                        |                                                                                                                                      
agcGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACt                                                                                                                                      <  1:270681/139‑1 (MQ=255)
  cGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACGATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGCATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGc                                                                                                                                    >  1:1159632/1‑139 (MQ=255)
         ttCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGAATCACTGCGGTaaaa                                                                                                                             <  2:1159632/139‑1 (MQ=255)
                 gTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACggg                                                                                                                     <  2:133042/139‑1 (MQ=255)
                       aaCGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGt                                                                                                               >  2:973359/1‑139 (MQ=255)
                           cTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgat                                                                                                           <  2:448403/139‑1 (MQ=255)
                            tCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatt                                                                                                          >  2:1262259/1‑139 (MQ=255)
                             cACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgattt                                                                                                         >  2:242923/1‑139 (MQ=255)
                                         tACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                             <  1:74951/139‑1 (MQ=255)
                                         tACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAACTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  2:1128004/128‑1 (MQ=255)
                                         tACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAACTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        >  1:1128004/1‑128 (MQ=255)
                                          aCGTGAAGGATGGAGTTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTCTGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAcc                                                                                            >  2:1158969/1‑139 (MQ=255)
                                          aCGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAcc                                                                                            >  2:405375/1‑139 (MQ=255)
                                              gAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        >  2:91010/1‑139 (MQ=255)
                                               aaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              >  2:1404603/1‑132 (MQ=255)
                                               aaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              <  1:1404603/132‑1 (MQ=255)
                                               aaGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                       <  2:1605871/139‑1 (MQ=255)
                                                    tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCTGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAt                                                                                  <  2:395017/139‑1 (MQ=255)
                                                    tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                       >  2:1258063/1‑134 (MQ=255)
                                                    tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                       <  1:1258063/134‑1 (MQ=255)
                                                     ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                                 >  1:533168/1‑139 (MQ=255)
                                                      gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  2:863115/1‑136 (MQ=255)
                                                      gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:863115/136‑1 (MQ=255)
                                                      gagaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAttt                                                                                >  1:446540/1‑139 (MQ=255)
                                                            aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCa                                                                          >  1:1401392/1‑139 (MQ=255)
                                                                tAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAGCCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTa                                                                      <  1:405375/139‑1 (MQ=255)
                                                                  aaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAtc                                                                             <  1:746093/130‑1 (MQ=255)
                                                                  aaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAtc                                                                             >  2:746093/1‑130 (MQ=255)
                                                                  aaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAAGATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACTGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    >  1:1103508/1‑139 (MQ=255)
                                                                       cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              >  1:743455/1‑108 (MQ=255)
                                                                       cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATaa                                                                                              <  2:743455/108‑1 (MQ=255)
                                                                       cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                               >  1:257357/1‑139 (MQ=255)
                                                                             ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:242923/139‑1 (MQ=255)
                                                                             ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  2:1010148/139‑1 (MQ=255)
                                                                                  cGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAtt                                                    <  1:973359/139‑1 (MQ=255)
                                                                                    gagaAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCt                                                  <  2:533168/139‑1 (MQ=255)
                                                                                    gagaAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCt                                                  >  1:840604/1‑139 (MQ=255)
                                                                                      gaAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTg                                                                                                                                     >  1:1512401/1‑54 (MQ=255)
                                                                                      gaAATTCCGGGGAGAATATAATACTTTCAGTGCGATATTTCTTATGCATCACTg                                                                                                                                     <  2:1512401/54‑1 (MQ=255)
                                                                                       aaaTTCCGGGGAGAACATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTaca                                               <  1:1158969/139‑1 (MQ=255)
                                                                                        aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  2:1401392/139‑1 (MQ=255)
                                                                                        aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  2:446540/139‑1 (MQ=255)
                                                                                           tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                              <  2:346389/104‑1 (MQ=255)
                                                                                           tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                              >  1:346389/1‑104 (MQ=255)
                                                                                               gggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              <  1:1556057/132‑1 (MQ=255)
                                                                                               gggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTacac                                              >  2:1556057/1‑132 (MQ=255)
                                                                                                ggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTa                                      <  2:257357/139‑1 (MQ=255)
                                                                                                              tttCAGTGCGATTTTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            <  2:71451/135‑1 (MQ=255)
                                                                                                              tttCAGTGCGATTTTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            >  1:71451/1‑135 (MQ=255)
                                                                                                              tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttgat                                                                                                      >  1:211235/1‑61 (MQ=255)
                                                                                                              tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttgat                                                                                                      <  2:211235/61‑1 (MQ=255)
                                                                                                              tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                   >  1:171551/1‑64 (MQ=255)
                                                                                                              tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                   <  2:171551/64‑1 (MQ=255)
                                                                                                                tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        <  1:1485118/89‑1 (MQ=255)
                                                                                                                tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        >  2:1485118/1‑89 (MQ=255)
                                                                                                                tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                      >  1:1480084/1‑139 (MQ=255)
                                                                                                                tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                      <  2:840604/139‑1 (MQ=255)
                                                                                                                tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                      >  1:1606999/1‑139 (MQ=255)
                                                                                                                     gCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGa                 >  2:1167321/1‑139 (MQ=255)
                                                                                                                     gCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGa                 <  1:1262259/139‑1 (MQ=255)
                                                                                                                       gATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                              >  1:439921/1‑76 (MQ=255)
                                                                                                                       gATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTAt                                                                              <  2:439921/76‑1 (MQ=255)
                                                                                                                        atatTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCa              <  1:1396386/139‑1 (MQ=255)
                                                                                                                           tttCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAg           <  2:1606999/139‑1 (MQ=255)
                                                                                                                                 aTGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGtt     >  1:457941/1‑139 (MQ=255)
                                                                                                                                    cATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGa  <  2:1480084/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099101‑2099371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A