Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A7 F1 I2 R1 | 766 | 64.2 | 3232166 | 90.9% | 2938038 | 104.5 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,365,284 | G→A | Q113* (CAG→TAG) | ais ← | putative LPS core heptose(II)‑phosphate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,365,284 | 0 | G | A | 100.0% | 213.7 / NA | 65 | Q113* (CAG→TAG) | ais | putative LPS core heptose(II)‑phosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/30); total (35/30) | |||||||||||
TGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTT > NC_000913/2365152‑2365417 | tGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGt > 2:905388/1‑139 (MQ=255) aaaaTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCTACTAAATGATcc > 2:445910/1‑139 (MQ=255) aaaaTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTcc > 1:395262/1‑139 (MQ=255) aaTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCgg < 1:874797/139‑1 (MQ=255) aTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGa < 1:905388/139‑1 (MQ=255) atatTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTAt < 2:293903/139‑1 (MQ=255) tCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAAt < 1:1110622/139‑1 (MQ=255) aGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATaa < 2:395262/139‑1 (MQ=255) gcgcTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg < 2:171086/118‑1 (MQ=255) gcgcTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg > 1:171086/1‑118 (MQ=255) ccTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa < 1:1262066/110‑1 (MQ=255) ccTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa > 2:1262066/1‑110 (MQ=255) gCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTaa < 2:1035454/139‑1 (MQ=255) ataAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCg < 2:635269/139‑1 (MQ=255) taAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa > 1:1104919/1‑96 (MQ=255) taAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa < 2:1104919/96‑1 (MQ=255) ctcATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc < 1:1199068/120‑1 (MQ=255) ctcATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc > 2:1199068/1‑120 (MQ=255) tACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc > 2:672824/1‑79 (MQ=255) tACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc < 1:672824/79‑1 (MQ=255) tACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTc > 1:310618/1‑139 (MQ=255) cACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACggg > 2:1376333/1‑139 (MQ=255) cACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACggg > 1:269501/1‑139 (MQ=255) tGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCAt < 2:310618/139‑1 (MQ=255) gaagaaGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATc < 1:1341485/139‑1 (MQ=255) aagaagTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc < 1:663901/69‑1 (MQ=255) aagaagTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc > 2:663901/1‑69 (MQ=255) aagaagTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATcc > 2:691160/1‑139 (MQ=255) agTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTggg < 1:1376333/139‑1 (MQ=255) tcatttATCTAGCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAACTGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc > 1:933231/4‑99 (MQ=255) tCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc < 2:933231/99‑1 (MQ=255) gTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc < 1:1268357/97‑1 (MQ=255) gTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc > 2:1268357/1‑97 (MQ=255) gTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTAcc > 1:398683/1‑139 (MQ=255) ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg > 2:646301/1‑56 (MQ=255) ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg < 1:646301/56‑1 (MQ=255) ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc < 1:1410192/95‑1 (MQ=255) ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc > 2:1410192/1‑95 (MQ=255) ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCtt > 2:531246/1‑139 (MQ=255) ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCtt > 1:1279691/1‑139 (MQ=255) cGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGa > 1:534937/1‑50 (MQ=255) cGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGa < 2:534937/50‑1 (MQ=255) tCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACgg < 2:795313/113‑1 (MQ=255) tCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACgg > 1:795313/1‑113 (MQ=255) tCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAAt > 1:462162/1‑139 (MQ=255) aaTTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGata < 2:1324013/81‑1 (MQ=255) aaTTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGata > 1:1324013/1‑81 (MQ=255) aTTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATAcc > 1:466849/1‑139 (MQ=255) gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAAt < 1:775777/49‑1 (MQ=255) gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAAt > 2:775777/1‑49 (MQ=255) gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc > 2:105745/1‑89 (MQ=255) gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc < 1:105745/89‑1 (MQ=255) gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTg > 2:1436759/1‑139 (MQ=255) cTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGa < 2:269501/139‑1 (MQ=255) cTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGa < 2:1279691/139‑1 (MQ=255) ggTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTggg < 1:1534838/98‑1 (MQ=255) ggTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTggg > 2:1534838/1‑98 (MQ=255) ggTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCattga > 1:114422/1‑139 (MQ=255) gTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCt > 1:1594922/1‑126 (MQ=255) gTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCt < 2:1594922/126‑1 (MQ=255) gTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCattgat > 1:1170417/1‑139 (MQ=255) ccGACTAAATGGTGCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGt > 1:1074789/1‑139 (MQ=255) ccGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc > 2:1054691/1‑73 (MQ=255) ccGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc < 1:1054691/73‑1 (MQ=255) cGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGtt > 2:1457861/1‑139 (MQ=255) | TGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTT > NC_000913/2365152‑2365417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |