Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
766 |
64.2 |
3232166 |
90.9% |
2938038 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,449,228 |
C→T |
*274* (TAG→TAA) |
yfcO ← |
DUF2544 family putative outer membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,449,228 | 0 | C | T | 100.0%
| 98.1
/ NA
| 31 | *274* (TAG→TAA) | yfcO | DUF2544 family putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/16); total (15/16) |
GCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCC > NC_000913/2449093‑2449363
|
gCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTAc < 2:1037707/139‑1 (MQ=255)
gcggcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAtt < 1:236969/139‑1 (MQ=255)
ggcgTTGGTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGAGTTACAACTCATTCa < 1:1079656/139‑1 (MQ=255)
attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCg < 2:759037/139‑1 (MQ=255)
aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAg > 2:681039/1‑139 (MQ=255)
aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAg > 1:277882/1‑139 (MQ=255)
gTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGt > 1:485844/1‑139 (MQ=255)
agagTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAAt < 2:277882/139‑1 (MQ=255)
gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTATGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa < 2:1614750/139‑1 (MQ=255)
cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa < 2:485844/139‑1 (MQ=255)
gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTgg > 2:1472842/1‑139 (MQ=255)
aggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg < 2:1138202/119‑1 (MQ=255)
aggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg > 1:1138202/1‑119 (MQ=255)
ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgc > 2:1350946/1‑139 (MQ=255)
cgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt > 2:1382739/1‑104 (MQ=255)
cgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt < 1:1382739/104‑1 (MQ=255)
tGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa < 1:233831/118‑1 (MQ=255)
tGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa > 2:233831/1‑118 (MQ=255)
cAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTta < 1:1472842/139‑1 (MQ=255)
ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTAc > 2:1503488/1‑37 (MQ=255)
ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTAc < 1:1503488/37‑1 (MQ=255)
gtgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa < 1:497308/66‑1 (MQ=255)
gtgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa > 2:497308/1‑66 (MQ=255)
tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCaa > 1:338390/1‑46 (MQ=255)
tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCaa < 2:338390/46‑1 (MQ=255)
tCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca < 1:798425/82‑1 (MQ=255)
tCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca > 2:798425/1‑82 (MQ=255)
cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt < 1:16112/105‑1 (MQ=255)
cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt > 2:16112/1‑105 (MQ=255)
cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc > 1:1058008/1‑139 (MQ=255)
gggTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTcc > 2:1336017/1‑139 (MQ=255)
|
GCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCC > NC_000913/2449093‑2449363
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
