Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
766 |
64.2 |
3232166 |
90.9% |
2938038 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,574,135 |
G→A |
D57D (GAC→GAT) |
eutT ← |
cobalamin adenosyltransferase involved in ethanolamine utilization |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,574,135 | 0 | G | A | 100.0%
| 105.0
/ NA
| 33 | D57D (GAC→GAT) | eutT | cobalamin adenosyltransferase involved in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/20); total (13/20) |
CCATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT > NC_000913/2573999‑2574269
|
ccATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCatcatc > 1:650815/1‑139 (MQ=255)
cATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTtcatcatca > 2:1227043/1‑139 (MQ=255)
tttttCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaaca < 1:1469234/139‑1 (MQ=255)
cgccgACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg < 1:1266014/139‑1 (MQ=255)
cgccgACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg < 1:568049/139‑1 (MQ=255)
tGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt < 1:1227043/139‑1 (MQ=255)
tGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt < 1:525110/139‑1 (MQ=255)
tCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAc > 2:967304/1‑138 (MQ=255)
tCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAc < 1:967304/138‑1 (MQ=255)
gACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAg < 2:650815/139‑1 (MQ=255)
aGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc > 2:794118/1‑135 (MQ=255)
aGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc < 1:794118/135‑1 (MQ=255)
ttCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTcc > 2:1094033/1‑139 (MQ=255)
agcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATgcg > 1:562334/1‑109 (MQ=255)
agcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATgcg < 2:562334/109‑1 (MQ=255)
ccTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg < 1:434410/139‑1 (MQ=255)
gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc < 1:448942/79‑1 (MQ=255)
gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc > 2:448942/1‑79 (MQ=255)
gTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCC‑GGCAGAGGGCGTCAGGCGa > 2:438665/1‑139 (MQ=255)
aTCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTg > 2:995029/1‑139 (MQ=255)
tCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGAc < 2:1577340/136‑1 (MQ=255)
tCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGAc > 1:1577340/1‑136 (MQ=255)
tCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGt < 1:1094033/139‑1 (MQ=255)
cGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg < 2:1538751/59‑1 (MQ=255)
cGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg > 1:1538751/1‑59 (MQ=255)
cTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCaa < 1:1085144/73‑1 (MQ=255)
cTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCaa > 2:1085144/1‑73 (MQ=255)
cTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAGACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCg < 2:283438/139‑1 (MQ=255)
gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTcc > 2:64363/1‑95 (MQ=255)
gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTcc < 1:64363/95‑1 (MQ=255)
ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAATTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTgcgc > 1:1067485/1‑139 (MQ=255)
ggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGc < 2:692571/139‑1 (MQ=255)
ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAATTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg > 1:929920/1‑110 (MQ=255)
ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg < 2:929920/110‑1 (MQ=255)
gctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGt < 1:995029/139‑1 (MQ=255)
tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg < 2:1177724/103‑1 (MQ=255)
tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg > 1:1177724/1‑103 (MQ=255)
catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt < 1:1374443/139‑1 (MQ=255)
catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt < 2:1067485/139‑1 (MQ=255)
|
CCATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT > NC_000913/2573999‑2574269
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
