Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A3 F1 I2 R1 | 777 | 59.2 | 2955938 | 92.5% | 2734242 | 106.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,574,135 | G→A | D57D (GAC→GAT) | eutT ← | cobalamin adenosyltransferase involved in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,574,135 | 0 | G | A | 100.0% | 111.4 / NA | 35 | D57D (GAC→GAT) | eutT | cobalamin adenosyltransferase involved in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/17); total (18/17) | |||||||||||
TTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT > NC_000913/2574004‑2574269 | ttttGCGCCAACAGGTGAGTCAGCGGATCCGGATTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCTTCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa < 1:77444/139‑1 (MQ=255) gccgACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAggc > 2:1016625/1‑139 (MQ=255) tGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt < 1:1016625/139‑1 (MQ=255) ccGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggcg < 2:572889/139‑1 (MQ=255) gTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTc > 2:533573/1‑139 (MQ=255) cagcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCggg < 2:409011/139‑1 (MQ=255) gcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCa < 2:606630/139‑1 (MQ=255) caCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCag < 1:533573/139‑1 (MQ=255) gCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAgg > 2:1365972/1‑139 (MQ=255) ccTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg < 2:270562/139‑1 (MQ=255) cTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGc > 2:783581/1‑139 (MQ=255) cTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGc > 1:555312/1‑139 (MQ=255) ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCG‑‑‑GCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGt > 1:493892/1‑74 (MQ=255) ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCG‑‑‑GCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGt < 2:493892/74‑1 (MQ=255) aTCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTg > 2:553350/1‑139 (MQ=255) gCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTcc > 2:1223965/1‑139 (MQ=255) cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg < 2:1319474/139‑1 (MQ=255) cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg > 1:164516/1‑54 (MQ=255) cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg < 2:164516/54‑1 (MQ=255) cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt < 1:1148651/56‑1 (MQ=255) cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt > 2:1148651/1‑56 (MQ=255) cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATgg < 1:171219/139‑1 (MQ=255) gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGcc < 2:1206793/46‑1 (MQ=255) gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGcc > 1:1206793/1‑46 (MQ=255) gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCaa < 1:821062/91‑1 (MQ=255) gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCaa > 2:821062/1‑91 (MQ=255) gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGAtctc > 1:987481/1‑139 (MQ=255) ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCa > 2:1402072/1‑67 (MQ=255) ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCa < 1:1402072/67‑1 (MQ=255) cggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg > 1:67012/1‑116 (MQ=255) cggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg < 2:67012/116‑1 (MQ=255) ggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGc > 2:114940/1‑139 (MQ=255) ctgctgTTCATCATCAACAAACAGGCGGCCCTGCt < 2:330514/35‑1 (MQ=255) ctgctgTTCATCATCAACAAACAGGCGGCCCTGCt > 1:330514/1‑35 (MQ=255) ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGGGc > 2:953697/1‑139 (MQ=255) tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGt < 1:114940/139‑1 (MQ=255) catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggcg < 1:907829/55‑1 (MQ=255) catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggcg > 2:907829/1‑55 (MQ=255) catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt < 1:990877/139‑1 (MQ=255) catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt < 1:553350/139‑1 (MQ=255) | TTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT > NC_000913/2574004‑2574269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |