Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,340,588 T→C L105S (TTA→TCA)  yrbG → putative calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,340,5880TC100.0% 203.5 / NA 57L105S (TTA→TCA) yrbGputative calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

CCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340480‑3340721
                                                                                                            |                                                                                                                                     
ccGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         <  2:827565/139‑1 (MQ=255)
    tGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGt                                                                                                     >  2:1278887/1‑139 (MQ=255)
              cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              >  1:1568117/1‑120 (MQ=255)
              cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              <  2:1568117/120‑1 (MQ=255)
              cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTActct                                                                                           >  2:720037/1‑139 (MQ=255)
                      aaaCATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGa                                                                                   <  1:1341115/139‑1 (MQ=255)
                                    tatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     <  1:720037/139‑1 (MQ=255)
                                       tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTa                                                                                          <  1:1429075/115‑1 (MQ=255)
                                       tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTa                                                                                          >  2:1429075/1‑115 (MQ=255)
                                       tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAAGTTAGTCGCAGCGa                                                                  >  2:1535237/1‑139 (MQ=255)
                                       tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGa                                                                  <  1:1278887/139‑1 (MQ=255)
                                        gctaatgagaggTCTGGCTGCGCAGGCTCGTCCTTTTACCGTACATTATGATGTTCTACGCCGTGAATCAACCTTAATGTTGTTGGTCAGAGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGAt                                                                 >  1:721630/11‑139 (MQ=255)
                                        gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGAt                                                                 >  2:685106/1‑139 (MQ=255)
                                          tGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCa                                                                                                                   >  1:1148855/1‑87 (MQ=255)
                                          tGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCa                                                                                                                   <  2:1148855/87‑1 (MQ=255)
                                          tGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATgg                                                               >  1:1488146/1‑139 (MQ=255)
                                            aTCGTCGGTCTGGCTGCGCCGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             >  1:1039116/1‑139 (MQ=255)
                                            aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             <  1:1375519/139‑1 (MQ=255)
                                            aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             >  2:1375519/1‑139 (MQ=255)
                                            aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             <  2:1460513/139‑1 (MQ=255)
                                            aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             <  2:1488146/139‑1 (MQ=255)
                                            aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             >  2:1094032/1‑139 (MQ=255)
                                              ccTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATc                                                           >  2:104504/1‑139 (MQ=255)
                                              ccTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATc                                                           >  1:623000/1‑139 (MQ=255)
                                               cTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCt                                                          <  2:623000/139‑1 (MQ=255)
                                                        gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCctct                                                 >  2:25679/1‑139 (MQ=255)
                                                           gcgcTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTc                                              <  2:1039116/139‑1 (MQ=255)
                                                             gcTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     >  1:682790/1‑114 (MQ=255)
                                                             gcTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     <  2:682790/114‑1 (MQ=255)
                                                             gcTGGTTCGTCCTTTTACCGTCCATTCTGATGTCCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              >  2:1545224/1‑73 (MQ=255)
                                                             gcTGGTTCGTCCTTTTACCGTCCATTCTGATGTCCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              <  1:1545224/73‑1 (MQ=255)
                                                                  ttCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCtgt                                       >  1:411651/1‑139 (MQ=255)
                                                                      tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  2:432771/1‑77 (MQ=255)
                                                                      tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:432771/77‑1 (MQ=255)
                                                                      tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTa                                   >  1:771748/1‑139 (MQ=255)
                                                                           ttACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:1001480/72‑1 (MQ=255)
                                                                           ttACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  2:1001480/1‑72 (MQ=255)
                                                                                 tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        >  1:400935/1‑139 (MQ=255)
                                                                                    aTTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGt                                                                          >  2:794130/1‑86 (MQ=255)
                                                                                    aTTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGt                                                                          <  1:794130/86‑1 (MQ=255)
                                                                                         gATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTa                <  1:104504/139‑1 (MQ=255)
                                                                                           tGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCtt                                                <  1:1550591/105‑1 (MQ=255)
                                                                                           tGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCtt                                                >  2:1550591/1‑105 (MQ=255)
                                                                                               cTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  1:458602/46‑1 (MQ=255)
                                                                                               cTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  2:458602/1‑46 (MQ=255)
                                                                                                    ccGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  2:162001/1‑47 (MQ=255)
                                                                                                    ccGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:162001/47‑1 (MQ=255)
                                                                                                     cGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtctc                                                     <  1:354330/90‑1 (MQ=255)
                                                                                                     cGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtctc                                                     >  2:354330/1‑90 (MQ=255)
                                                                                                       tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        <  1:1206532/53‑1 (MQ=255)
                                                                                                       tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        >  2:1206532/1‑53 (MQ=255)
                                                                                                       tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     >  1:642057/1‑72 (MQ=255)
                                                                                                       tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                                     <  2:642057/72‑1 (MQ=255)
                                                                                                       tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  1:766056/1‑139 (MQ=255)
                                                                                                       tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  1:212787/1‑139 (MQ=255)
                                                                                                         aaTCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     >  2:514300/1‑54 (MQ=255)
                                                                                                         aaTCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     <  1:514300/54‑1 (MQ=255)
                                                                                                            |                                                                                                                                     
CCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340480‑3340721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A