Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,588	T→C	L105S (TTA→TCA)	yrbG →	putative calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,588	0	T	C	100.0%	188.7 / NA	52	L105S (TTA→TCA)	yrbG	putative calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/24); total (28/24)

TCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340455‑3340721

tctcTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCt                                                                                                                                    >  2:837825/1‑139 (MQ=255)

          aacaacGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATgttgtt                                                                                                                          <  1:396775/139‑1 (MQ=255)

                    tttAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                                <  1:738693/139‑1 (MQ=255)

                           gTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                           <  1:23536/137‑1 (MQ=255)

                           gTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                           >  2:23536/1‑137 (MQ=255)

                                 aCAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                   <  1:186348/139‑1 (MQ=255)

                                       cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                         >  1:772601/1‑127 (MQ=255)

                                       cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGACTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                         <  2:772601/127‑1 (MQ=255)

                                         cGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCTGGTTCCGTA‑‑CTCTAt                                                                                         >  1:428091/1‑139 (MQ=255)

                                          ggCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                         <  2:519083/124‑1 (MQ=255)

                                          ggCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                         >  1:519083/1‑124 (MQ=255)

                                                      aTCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                   <  1:215758/118‑1 (MQ=255)

                                                      aTCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                   >  2:215758/1‑118 (MQ=255)

                                                           tatatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                           >  1:799963/1‑105 (MQ=255)

                                                           tatatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                           <  2:799963/105‑1 (MQ=255)

                                                           tatatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGc                                                                       >  2:1044346/1‑139 (MQ=255)

                                                             tatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGttgttg                                                                                                                         <  1:820777/89‑1 (MQ=255)

                                                             tatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGttgttg                                                                                                                         >  2:820777/1‑89 (MQ=255)

                                                               ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                                <  2:766772/96‑1 (MQ=255)

                                                               ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                                >  1:766772/1‑96 (MQ=255)

                                                               ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTCTATGACGGACAACTTAGTCGCAg                                                                     >  1:817514/1‑139 (MQ=255)

                                                               ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGc                                                                    <  2:30970/138‑1 (MQ=255)

                                                               ttGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGc                                                                    >  1:30970/1‑138 (MQ=255)

                                                                 gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCAc                                                                                                                                       >  1:230317/1‑71 (MQ=255)

                                                                     caccTCGATCTGGCTGCGCTGGTTCGATATTTTACCGCCCATTCTGATGATCTGTGCCGTGAATCACCCTTAAATTTGTTGGTCAGCGTGGTTGACGCTTCCGTA‑‑CTCTATGACAGGCAACTTAGTCGCAGCGTTGGTa                                                             >  1:111659/3‑139 (MQ=255)

                                                                     aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTcc                                                                                                    >  2:921641/1‑102 (MQ=255)

                                                                     aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTcc                                                                                                    <  1:921641/102‑1 (MQ=255)

                                                                     aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTa                                                             >  1:212765/1‑139 (MQ=255)

                                                                               tGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGa                                                                  >  1:663081/1‑124 (MQ=255)

                                                                               tGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGa                                                                  <  2:663081/124‑1 (MQ=255)

                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                   >  1:804126/1‑91 (MQ=255)

                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                   <  2:804126/91‑1 (MQ=255)

                                                                                     cgcTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAg                                                                     <  1:895543/115‑1 (MQ=255)

                                                                                     cgcTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAg                                                                     >  2:895543/1‑115 (MQ=255)

                                                                                       cTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTgg                                           >  2:1020766/1‑139 (MQ=255)

                                                                                       cTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTgg                                           <  1:837825/139‑1 (MQ=255)

                                                                                        tGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGc                                          <  1:368223/139‑1 (MQ=255)

                                                                                                ccTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTAt                                  <  1:390645/139‑1 (MQ=255)

                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        >  1:1013536/1‑139 (MQ=255)

                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        <  1:1020766/139‑1 (MQ=255)

                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGACAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGTCGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        >  2:766493/1‑139 (MQ=255)

                                                                                                            cATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGc                                     <  2:752410/124‑1 (MQ=255)

                                                                                                            cATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGc                                     >  1:752410/1‑124 (MQ=255)

                                                                                                                         tACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCTGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGc         <  2:428091/139‑1 (MQ=255)

                                                                                                                             ccGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        <  1:285262/120‑1 (MQ=255)

                                                                                                                             ccGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        >  2:285262/1‑120 (MQ=255)

                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAg                                                                     <  2:528003/72‑1 (MQ=255)

                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAg                                                                     >  1:528003/1‑72 (MQ=255)

                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGgctg                             <  1:1195508/112‑1 (MQ=255)

                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGgctg                             >  2:1195508/1‑112 (MQ=255)

                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  1:638251/1‑139 (MQ=255)

                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  2:850934/1‑139 (MQ=255)

TCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTA‑‑CTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340455‑3340721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A