Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,934	A→G	Y143H (TAC→CAC)	psd ←	phosphatidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,934	0	A	G	100.0%	166.1 / NA	48	Y143H (TAC→CAC)	psd	phosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/21); total (27/21)

ATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGA  >  NC_000913/4389804‑4390065

aTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcgcg                                                                                                                             <  2:144477/139‑1 (MQ=255)

           gttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                  <  1:1468208/139‑1 (MQ=255)

                   cAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAg                                                                                                                 <  2:456328/132‑1 (MQ=255)

                   cAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAg                                                                                                                 >  1:456328/1‑132 (MQ=255)

                   cAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGACCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTcac                                                                                                          >  1:963822/1‑139 (MQ=255)

                     aaCAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTGCGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACaa                                                                                                        >  1:820164/1‑139 (MQ=255)

                            tCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                            >  2:514408/1‑112 (MQ=255)

                            tCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                            <  1:514408/112‑1 (MQ=255)

                                    ttCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGa                                                                                         >  1:93183/1‑139 (MQ=255)

                                           ccGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                  <  2:963822/139‑1 (MQ=255)

                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                               <  1:1069140/139‑1 (MQ=255)

                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                               >  2:751579/1‑139 (MQ=255)

                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                               >  1:421207/1‑139 (MQ=255)

                                                     gTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                  >  2:1014768/1‑97 (MQ=255)

                                                     gTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                  <  1:1014768/97‑1 (MQ=255)

                                                             agaagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                             >  1:353536/1‑110 (MQ=255)

                                                             agaagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                             <  2:353536/110‑1 (MQ=255)

                                                                agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTa                                                                                                                   >  2:569460/1‑85 (MQ=255)

                                                                agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTa                                                                                                                   <  1:569460/85‑1 (MQ=255)

                                                                   gaTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcag                                                          <  2:820164/139‑1 (MQ=255)

                                                                    aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGTGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagc                                                         >  2:85072/1‑139 (MQ=255)

                                                                       gCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGga                                                                                                                         >  2:312131/1‑72 (MQ=255)

                                                                       gCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGga                                                                                                                         <  1:312131/72‑1 (MQ=255)

                                                                       gCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAgg                                                      >  1:236702/1‑139 (MQ=255)

                                                                                   gATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                          <  1:85072/139‑1 (MQ=255)

                                                                                            aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                                <  1:1566856/44‑1 (MQ=255)

                                                                                            aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                                >  2:1566856/1‑44 (MQ=255)

                                                                                            aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                  >  1:546231/1‑90 (MQ=255)

                                                                                            aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                  <  2:546231/90‑1 (MQ=255)

                                                                                              gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGcc                               >  1:1399408/1‑139 (MQ=255)

                                                                                               cAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCt                              <  2:236702/139‑1 (MQ=255)

                                                                                                aGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCtt                             >  1:1253550/1‑139 (MQ=255)

                                                                                                 gAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCttt                            >  2:755074/1‑139 (MQ=255)

                                                                                                    tACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATGGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGc                         <  1:673060/139‑1 (MQ=255)

                                                                                                        gTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTc                                                 >  2:16927/1‑111 (MQ=255)

                                                                                                        gTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTc                                                 <  1:16927/111‑1 (MQ=255)

                                                                                                          tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCCGCCAGCAGGGCTTc                                                 >  1:1197317/1‑109 (MQ=255)

                                                                                                          tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCCGCCAGCAGGGCTTc                                                 <  2:1197317/109‑1 (MQ=255)

                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa                 >  2:376170/1‑139 (MQ=255)

                                                                                                              cGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACATACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCATGCCAGCAGGGCTTCGAGGCTGTAGTtg                                    <  2:999720/118‑1 (MQ=255)

                                                                                                              cGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTc                                                 >  2:25739/1‑105 (MQ=255)

                                                                                                              cGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTc                                                 <  1:25739/105‑1 (MQ=255)

                                                                                                              cGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTtg                                    >  1:999720/1‑118 (MQ=255)

                                                                                                                   tgtgTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTAtc          >  1:830809/1‑139 (MQ=255)

                                                                                                                           cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGACCAGGTCCGCCATCAGATAGTTGCCTGCCACCAGGGCTTCGGGGCTGTCGTTGTGGCCTTTGGCTTGCAGGGTTTTATCTTCTTCgc  >  1:149436/1‑138 (MQ=255)

                                                                                                                           cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGa  >  1:794627/1‑139 (MQ=255)

                                                                                                                           cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGCtgtctcttatac                          >  2:690582/1‑106 (MQ=255)

                                                                                                                           cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGCtgtctcttatac                          <  1:690582/115‑10 (MQ=255)

                                                                                                                               ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATcttctt     <  2:1615320/132‑1 (MQ=255)

                                                                                                                               ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGCTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAgg                  <  2:993983/119‑1 (MQ=255)

ATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGA  >  NC_000913/4389804‑4390065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A