Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,934	A→G	Y143H (TAC→CAC)	psd ←	phosphatidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,934	0	A	G	100.0%	158.1 / NA	44	Y143H (TAC→CAC)	psd	phosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/17); total (27/17)

CAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGAT  >  NC_000913/4389801‑4390066

cAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                                 <  1:134014/139‑1 (MQ=255)

            tcgttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGt                                                                                                                     <  2:309947/139‑1 (MQ=255)

               ttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAg                                                                                                                  >  2:285954/1‑139 (MQ=255)

                    ggCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTc                                                                                                             >  2:1018984/1‑139 (MQ=255)

                      cAAACAGATTCGGCACGTACTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTcac                                                                                                           >  2:585963/1‑139 (MQ=255)

                              ttCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                   <  2:218498/139‑1 (MQ=255)

                               tCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAcc                                                                                                  >  2:382906/1‑139 (MQ=255)

                                   cagggtCTGAGACGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                              >  2:613206/6‑139 (MQ=255)

                                                 tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGt                                                                                                                 >  2:749651/1‑106 (MQ=255)

                                                 tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGt                                                                                                                 <  1:749651/106‑1 (MQ=255)

                                                 tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                                >  2:989694/1‑139 (MQ=255)

                                                    gaTGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCAt                                                                             <  2:2602/139‑1 (MQ=255)

                                                           aaCGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAg                                                                      >  2:106271/1‑139 (MQ=255)

                                                            aCGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCggg                                                                                                                            >  1:1002080/1‑84 (MQ=255)

                                                            aCGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCggg                                                                                                                            <  2:1002080/84‑1 (MQ=255)

                                                              ggAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtg                                                                   <  1:653949/139‑1 (MQ=255)

                                                               gagaAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgc                                                                  >  2:415750/1‑139 (MQ=255)

                                                                  aagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcctg                                                               >  2:199418/1‑139 (MQ=255)

                                                                  aagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCATTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcctg                                                               >  1:904240/1‑139 (MQ=255)

                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgggg                                                                                                                           >  2:34527/1‑74 (MQ=255)

                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgggg                                                                                                                           <  1:34527/74‑1 (MQ=255)

                                                                       aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagc                                                          <  2:197594/139‑1 (MQ=255)

                                                                          gCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAgg                                                       >  1:565940/1‑139 (MQ=255)

                                                                              ggCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCtt                                                   <  1:585963/139‑1 (MQ=255)

                                                                                          atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGt                                       >  1:359329/1‑139 (MQ=255)

                                                                                                 gCAGAATACTGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTAGAGGCTg                                           >  1:708916/1‑128 (MQ=255)

                                                                                                 gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                           <  2:708916/128‑1 (MQ=255)

                                                                                                  cAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCt                               <  1:285954/139‑1 (MQ=255)

                                                                                                     aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCATCAGAGCTTCGAGGCTGTAGGTGTGGCCTTTg                            >  2:1003368/1‑139 (MQ=255)

                                                                                                     aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTg                            >  2:308683/1‑139 (MQ=255)

                                                                                                          cGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTg                       <  1:613206/139‑1 (MQ=255)

                                                                                                          cGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTg                       <  1:199418/139‑1 (MQ=255)

                                                                                                            ttGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCa                     <  1:1018984/139‑1 (MQ=255)

                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa                  >  1:424529/1‑139 (MQ=255)

                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa                  >  1:591648/1‑139 (MQ=255)

                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa                  >  1:325766/1‑139 (MQ=255)

                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa                  >  2:68229/1‑139 (MQ=255)

                                                                                                               cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa                  >  2:571952/1‑139 (MQ=255)

                                                                                                                 cGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGAtt                >  2:1001700/1‑139 (MQ=255)

                                                                                                                    cATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTa             <  1:1001700/139‑1 (MQ=255)

                                                                                                                       gtgtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                   >  1:10445/1‑50 (MQ=255)

                                                                                                                       gtgtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                   <  2:10445/50‑1 (MQ=255)

                                                                                                                       gtgtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTAtct          >  2:1008008/1‑139 (MQ=255)

                                                                                                                         gtACGCGGTGGTGGTCACGCGGGGAGAGGGAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTAtcttc        >  2:462909/1‑139 (MQ=255)

                                                                                                                               ggtggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGAt  <  2:1003830/139‑1 (MQ=255)

                                                                                                                                  ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGa                                                >  2:692410/1‑90 (MQ=255)

CAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGAT  >  NC_000913/4389801‑4390066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A