Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 424,700 C→T A73A (GCG→GCA)  acpH ← acyl carrier protein (ACP) phosphodiesterase, ACP hydrolyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913424,7000CT100.0% 68.4 / NA 23A73A (GCG→GCA) acpHacyl carrier protein (ACP) phosphodiesterase, ACP hydrolyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

TGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCA  >  NC_000913/424571‑424829
                                                                                                                                 |                                                                                                                                 
tGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACgcgg                                                                                                                          <  2:860676/139‑1 (MQ=255)
       ggCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGttt                                                                                                                   <  1:508822/139‑1 (MQ=255)
            aaTCGTCATCACTTGCTCGCGGACATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTa                                                                                                              <  1:306774/139‑1 (MQ=255)
                                                   cTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTg                                                                       <  1:43517/139‑1 (MQ=255)
                                                     gTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTgtc                                                                     <  2:353642/139‑1 (MQ=255)
                                                         cGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGa                                                                          >  2:93551/1‑130 (MQ=255)
                                                         cGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGa                                                                          <  1:93551/130‑1 (MQ=255)
                                                          cggaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTgtcagtca                                                                >  1:855741/4‑139 (MQ=255)
                                                                  cGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcg                                                        >  1:314067/1‑139 (MQ=255)
                                                                   ggCTACAGCTGCGACCAGTGGCGGGAAATGAAGTAAACCCACATGGCTTCCAGCTTACTGGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGTTTGTCAGTCAagccgtcga                                                       <  1:776528/139‑1 (MQ=255)
                                                                              cGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                        <  2:971515/79‑1 (MQ=255)
                                                                              cGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                        >  1:971515/1‑79 (MQ=255)
                                                                                       gCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATg                                   <  1:118897/139‑1 (MQ=255)
                                                                                       gCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCAGCGCGTTTCAATACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATg                                   <  2:5320/139‑1 (MQ=255)
                                                                                         gggAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATgcc                                 <  2:314067/139‑1 (MQ=255)
                                                                                          ggAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATgcca                                >  2:618685/1‑139 (MQ=255)
                                                                                                          cacaTGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATgccagcca                            >  2:645194/1‑127 (MQ=255)
                                                                                                          cacaTGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATgccagcca                            <  1:645194/127‑1 (MQ=255)
                                                                                                          cacaTGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCg                >  1:962769/1‑139 (MQ=255)
                                                                                                            caTGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGa              >  1:1036337/1‑139 (MQ=255)
                                                                                                                       gCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcgatacgt                                                  <  1:959394/92‑1 (MQ=255)
                                                                                                                       gCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcgatacgt                                                  >  2:959394/1‑92 (MQ=255)
                                                                                                                        cGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCa  >  2:612403/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                 
TGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCA  >  NC_000913/424571‑424829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A