Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	716,388	C→T	pseudogene (210/651 nt)	ybfG ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	716,388	0	C	T	100.0%	163.0 / NA	51	pseudogene (210/651 nt)	ybfG	pseudogene
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/21); total (30/21)

TTAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAACTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTT  >  NC_000913/716257‑716520

ttAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCAt                                                                                                                               <  2:943170/139‑1 (MQ=255)

        aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                       >  1:1035500/1‑139 (MQ=255)

        aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                       >  1:805270/1‑139 (MQ=255)

        aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                       >  2:208385/1‑139 (MQ=255)

                    tGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCg                                                                                                           <  1:1163694/139‑1 (MQ=255)

                    tGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCg                                                                                                           <  2:1035500/139‑1 (MQ=255)

                     gCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCgg                                                                                                          <  1:768522/139‑1 (MQ=255)

                      cGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGa                                                                                                         >  1:24914/1‑139 (MQ=255)

                            cGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATa                                                                                                   >  2:601240/1‑139 (MQ=255)

                                   gATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAgc                                                                                            <  1:605423/139‑1 (MQ=255)

                                           aaGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCAt                                                                                    <  1:208385/139‑1 (MQ=255)

                                                  cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:990853/1‑139 (MQ=255)

                                                  cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:884129/1‑139 (MQ=255)

                                                  cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:836399/1‑139 (MQ=255)

                                                  cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:1080347/1‑139 (MQ=255)

                                                      ccTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCCCCGGAGCCATAGACAAGCGCACCCATCGGTGATTttg                                                                         <  1:942834/139‑1 (MQ=255)

                                                             aTTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCAc                                                                  <  2:1080347/139‑1 (MQ=255)

                                                                       ttGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGt                                                                                <  1:959001/115‑1 (MQ=255)

                                                                       ttGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGt                                                                                >  2:959001/1‑115 (MQ=255)

                                                                         gtgtCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATa                                                      >  1:454570/1‑139 (MQ=255)

                                                                                    aCTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGttt                                           >  2:449142/1‑139 (MQ=255)

                                                                                    aCTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGttt                                           >  2:344278/1‑139 (MQ=255)

                                                                                    aCTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGttt                                           >  2:338100/1‑139 (MQ=255)

                                                                                         aTCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      <  2:884129/139‑1 (MQ=255)

                                                                                         aTCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      <  1:338100/139‑1 (MQ=255)

                                                                                           ctcGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAg                                                                                                        <  1:256995/71‑1 (MQ=255)

                                                                                           ctcGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAg                                                                                                        >  2:256995/1‑71 (MQ=255)

                                                                                             cGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAAt                                  >  2:1004627/1‑139 (MQ=255)

                                                                                                ggCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCtt                               >  1:22833/1‑139 (MQ=255)

                                                                                                  cAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAgcgc                                                                                          >  2:928164/1‑78 (MQ=255)

                                                                                                  cAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAgcgc                                                                                          <  1:928164/78‑1 (MQ=255)

                                                                                                    aCCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTttatt                           >  2:1141810/1‑139 (MQ=255)

                                                                                                           gTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGt                    <  1:1141810/139‑1 (MQ=255)

                                                                                                               ccAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAaga                <  2:805270/139‑1 (MQ=255)

                                                                                                                   aTCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAagag               <  1:736674/136‑1 (MQ=255)

                                                                                                                   aTCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAagag               >  2:736674/1‑136 (MQ=255)

                                                                                                                   aTCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTc            >  2:958570/1‑139 (MQ=255)

                                                                                                                      cTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGt         <  1:449142/139‑1 (MQ=255)

                                                                                                                        gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      <  2:693358/108‑1 (MQ=255)

                                                                                                                        gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      >  1:693358/1‑108 (MQ=255)

                                                                                                                        gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTAt       >  1:104833/1‑139 (MQ=255)

                                                                                                                        gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTAt       >  1:1182406/1‑139 (MQ=255)

                                                                                                                          tATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCt                                                                >  2:328346/1‑80 (MQ=255)

                                                                                                                          tATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCt                                                                <  1:328346/80‑1 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAtt                                                                            >  2:436328/1‑65 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAtt                                                                            <  1:436328/65‑1 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTaa                         >  2:648572/1‑116 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTaa                         <  1:648572/116‑1 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt  >  2:657696/1‑139 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt  >  1:183000/1‑139 (MQ=255)

                                                                                                                             gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt  >  2:11676/1‑139 (MQ=255)

TTAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAACTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTT  >  NC_000913/716257‑716520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A