Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,315,402	C→T	L123L (CTG→CTA)	yciF ←	putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,315,402	0	C	T	100.0%	102.0 / NA	32	L123L (CTG→CTA)	yciF	putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16)

ATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCC  >  NC_000913/1315267‑1315537

atTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTcagctagc                                                                                                                                      <  1:40891/139‑1 (MQ=255)

       gCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               >  1:231741/1‑139 (MQ=255)

        cTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTaa                                                                                                                              <  2:66729/139‑1 (MQ=255)

             ttttCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTcc                                                                                                                         <  1:142599/139‑1 (MQ=255)

                      ttCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTgg                                                                                                                <  1:799164/139‑1 (MQ=255)

                       tCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGc                                                                                                               >  1:514749/1‑139 (MQ=255)

                              tACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCa                                                                                                        <  2:231741/139‑1 (MQ=255)

                                  ttattaATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               <  1:1153629/112‑1 (MQ=255)

                                  ttattaATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               >  2:1153629/1‑112 (MQ=255)

                                                     tcagtTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGtgctgc                                                                                 >  1:476236/1‑139 (MQ=255)

                                                          ttGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCaa                                                                            >  1:939772/1‑139 (MQ=255)

                                                           tGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           <  2:476236/139‑1 (MQ=255)

                                                           tGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           <  2:514749/139‑1 (MQ=255)

                                                                   gTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCgg                                                                   >  2:345631/1‑139 (MQ=255)

                                                                   gTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCgg                                                                   >  1:894385/1‑139 (MQ=255)

                                                                                     aGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               >  2:947391/1‑61 (MQ=255)

                                                                                     aGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               <  1:947391/61‑1 (MQ=255)

                                                                                      gggTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGttttt                                                >  1:1075170/1‑139 (MQ=255)

                                                                                           tctttcAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCa                                                                                                        <  1:774495/78‑1 (MQ=255)

                                                                                           tctttcAGAAGCTTCGCTGCTTTACGGTAACCTAATAGTTCAGCTAGAGTCGCTAATGTCCCGAAACTGGCAATCTCa                                                                                                        >  2:774495/1‑78 (MQ=255)

                                                                                              ttcAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTAc                                        <  2:1075170/139‑1 (MQ=255)

                                                                                                      cTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGc                                                                                                               >  1:858436/1‑60 (MQ=255)

                                                                                                      cTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGc                                                                                                               <  2:858436/60‑1 (MQ=255)

                                                                                                        tCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATg                                <  2:36270/137‑1 (MQ=255)

                                                                                                        tCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATg                                >  1:36270/1‑137 (MQ=255)

                                                                                                        tCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGAc                              >  2:1166679/1‑139 (MQ=255)

                                                                                                            tgctTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCa                          <  2:123006/139‑1 (MQ=255)

                                                                                                                 tACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGc                     <  1:690052/139‑1 (MQ=255)

                                                                                                                        aCCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCa              >  1:419018/1‑139 (MQ=255)

                                                                                                                                gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           >  2:889055/1‑70 (MQ=255)

                                                                                                                                gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           <  1:889055/70‑1 (MQ=255)

                                                                                                                                gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGc                                                           <  2:417782/86‑1 (MQ=255)

                                                                                                                                gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGc                                                           >  1:417782/1‑86 (MQ=255)

                                                                                                                                    agctagcGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTtct                                              <  1:896068/95‑1 (MQ=255)

                                                                                                                                    agctagcGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTcc  >  1:493453/1‑139 (MQ=255)

ATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCC  >  NC_000913/1315267‑1315537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A