Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,403,912 C→T V218V (GTG→GTA)  abgA ← p‑aminobenzoyl‑glutamate hydrolase, A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,403,9120CT100.0% 142.8 / NA 44V218V (GTG→GTA) abgAp‑aminobenzoyl‑glutamate hydrolase, A subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)

CAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGTTTGATGACG  >  NC_000913/1403782‑1404047
                                                                                                                                  |                                                                                                                                       
cAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGcc                                                                                                                                 <  2:337606/139‑1 (MQ=255)
      tGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGcc                                                                                                                                 <  1:650309/133‑1 (MQ=255)
      tGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGACAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGcc                                                                                                                                 >  2:650309/1‑133 (MQ=255)
        gCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGt                                                                                                                          >  2:655646/1‑138 (MQ=255)
        gCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGt                                                                                                                          <  1:655646/138‑1 (MQ=255)
        gCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTa                                                                                                                         >  1:671145/1‑139 (MQ=255)
        gCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTa                                                                                                                         >  1:722251/1‑139 (MQ=255)
           tgtgCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGc                                                                                                                      <  2:671145/139‑1 (MQ=255)
             tgctgcCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAgg                                                                                                                           >  2:659155/1‑132 (MQ=255)
             tgctgcCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAgg                                                                                                                           <  1:659155/132‑1 (MQ=255)
                   cgtcaacaaGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCa                                                                                                              <  1:744777/136‑1 (MQ=255)
                   cgccAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCa                                                                                                              <  1:980046/139‑1 (MQ=255)
                                       cGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCa                                                                                                       >  2:524240/1‑126 (MQ=255)
                                       cGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCa                                                                                                       <  1:524240/126‑1 (MQ=255)
                                       cGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACgg                                                                                                    <  1:735948/129‑1 (MQ=255)
                                       cGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACgg                                                                                                    >  2:735948/1‑129 (MQ=255)
                                       cGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACAGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAaata                                                                                          >  2:918767/1‑139 (MQ=255)
                                         tcttctGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGt                                                                                                                          >  1:660673/1‑105 (MQ=255)
                                         tcttctGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGt                                                                                                                          <  2:660673/105‑1 (MQ=255)
                                          cttctGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATc                                                                                       >  1:160336/1‑139 (MQ=255)
                                                      cgcCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTAc                                                                           <  2:722251/139‑1 (MQ=255)
                                                      cgcCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACAGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTAc                                                                           <  1:918767/139‑1 (MQ=255)
                                                            cGTGAGCGGCGGTACCGGTGAAGTGCCCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACAcc                                                                     <  1:571001/139‑1 (MQ=255)
                                                                aGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCa                                                                 >  2:752748/1‑139 (MQ=255)
                                                                     cggTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAAca                                                                                   >  1:536457/1‑116 (MQ=255)
                                                                     cggTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAAca                                                                                   <  2:536457/116‑1 (MQ=255)
                                                                     cggTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTAc                                                                           <  2:225299/124‑1 (MQ=255)
                                                                     cggTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTAc                                                                           >  1:225299/1‑124 (MQ=255)
                                                                                 aGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCcgcgc                                                >  2:641616/1‑139 (MQ=255)
                                                                                    gcgcGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGccc                                                                                                                                <  1:478903/56‑1 (MQ=255)
                                                                                    gcgcGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGccc                                                                                                                                >  2:478903/1‑56 (MQ=255)
                                                                                    gcgcGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGcc                                             >  1:1045734/1‑139 (MQ=255)
                                                                                     cgcgTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCa                                            >  2:760614/1‑139 (MQ=255)
                                                                                     cgcgTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCa                                            <  1:641616/139‑1 (MQ=255)
                                                                                       cgTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATc                                                                                       >  1:94819/1‑94 (MQ=255)
                                                                                       cgTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATc                                                                                       <  2:94819/94‑1 (MQ=255)
                                                                                          cAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGt                                       <  1:752748/139‑1 (MQ=255)
                                                                                           aaaTTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTa                                      <  1:760614/139‑1 (MQ=255)
                                                                                             aTTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCAc                                                                                                      <  1:830622/73‑1 (MQ=255)
                                                                                             aTTTGGTGGTTGCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCAc                                                                                                      >  2:830622/1‑73 (MQ=255)
                                                                                                        gCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAaataa                                                                                         >  2:430406/1‑75 (MQ=255)
                                                                                                        gCCATAAAATTATCACTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAaataa                                                                                         <  1:430406/75‑1 (MQ=255)
                                                                                                                       cTGCCGCACACTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGtt          >  1:1160284/1‑139 (MQ=255)
                                                                                                                              acacTACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGTTTGATGAc   <  2:160336/139‑1 (MQ=255)
                                                                                                                               cactacGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGTTTGATGACg  <  2:808982/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGTTTGATGACG  >  NC_000913/1403782‑1404047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A