Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A5 F1 I1 R1 | 770 | 51.4 | 2439994 | 93.8% | 2288714 | 110.3 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,766,131 | T→C | N852S (AAC→AGC) | ydiJ ← | putative FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,766,131 | 0 | T | C | 100.0% | 149.9 / NA | 42 | N852S (AAC→AGC) | ydiJ | putative FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/19); total (23/19) | |||||||||||
CCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACA > NC_000913/1766012‑1766262 | ccAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCtt > 2:233790/1‑139 (MQ=255)ccAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCtt > 1:1163155/1‑139 (MQ=255) cGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGt < 1:1168852/139‑1 (MQ=255) tCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCaa > 1:864014/1‑139 (MQ=255) tCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCaa < 1:233790/139‑1 (MQ=255) aTCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTg > 2:562048/1‑139 (MQ=255) tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCt > 1:1025041/1‑85 (MQ=255) tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCt < 2:1025041/85‑1 (MQ=255) tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCtt > 2:1094068/1‑98 (MQ=255) tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCtt < 1:1094068/98‑1 (MQ=255) cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAAt > 2:969622/1‑75 (MQ=255) cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAAt < 1:969622/75‑1 (MQ=255) cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGcc > 2:283313/1‑139 (MQ=255) cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGcc > 2:572094/1‑139 (MQ=255) cAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGt < 1:880078/86‑1 (MQ=255) cAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGt > 2:880078/1‑86 (MQ=255) aGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGaaa < 1:283313/139‑1 (MQ=255) tACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAAt < 1:572094/139‑1 (MQ=255) aGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAAt < 1:1069111/135‑1 (MQ=255) aGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAAt > 2:1069111/1‑135 (MQ=255) ccGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTaa > 2:152415/1‑139 (MQ=255) ggATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAaca > 1:728874/1‑139 (MQ=255) ccaccaTTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGaa < 2:1163155/139‑1 (MQ=255) cATACCCAGCTTCGCCATACGGCTGAGGAAATccgcc > 2:567656/1‑37 (MQ=255) cATACCCAGCTTCGCCATACGGCTGAGGAAATccgcc < 1:567656/37‑1 (MQ=255) cATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaa < 1:214590/71‑1 (MQ=255) cATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaa > 2:214590/1‑71 (MQ=255) tACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTa < 1:645082/77‑1 (MQ=255) tACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTa > 2:645082/1‑77 (MQ=255) cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa > 2:1180814/1‑68 (MQ=255) cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa < 1:1180814/68‑1 (MQ=255) cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa < 1:620996/66‑1 (MQ=255) cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa > 2:620996/1‑66 (MQ=255) cTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGaaa > 2:1215007/1‑98 (MQ=255) cTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGaaa < 1:1215007/98‑1 (MQ=255) cTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGa > 2:333081/1‑139 (MQ=255) ttCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGAc > 2:1171547/1‑139 (MQ=255) cGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa > 2:1021396/1‑60 (MQ=255) cGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa < 1:1021396/60‑1 (MQ=255) cATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACa < 2:864014/139‑1 (MQ=255) tACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa < 2:118784/53‑1 (MQ=255) tACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa > 1:118784/1‑53 (MQ=255) | CCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACA > NC_000913/1766012‑1766262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |