Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,971,902	T→C	T264A (ACC→GCC)	tar ←	methyl‑accepting chemotaxis protein II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,971,902	0	T	C	100.0%	99.0 / NA	29	T264A (ACC→GCC)	tar	methyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/15); total (14/15)

GCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGG  >  NC_000913/1971767‑1972037

gctCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCgtc                                                                                                                                      >  2:258704/1‑139 (MQ=255)

           gcggcAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAagag                                                                                                                            <  1:1136179/138‑1 (MQ=255)

           gcggcAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAagag                                                                                                                            >  2:1136179/1‑138 (MQ=255)

           gcggcAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGc                                                                                                                           >  2:477084/1‑139 (MQ=255)

              gcAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGtt                                                                                                                        <  1:756691/139‑1 (MQ=255)

               cAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTg                                                                                                                       <  2:936243/139‑1 (MQ=255)

                  tttCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCat                                                                                                                    <  1:231463/139‑1 (MQ=255)

                  tttCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCat                                                                                                                    <  2:672029/139‑1 (MQ=255)

                          aGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGaaa                                                                                                            <  1:258704/139‑1 (MQ=255)

                              cgGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGct                                                                                                        >  1:519176/1‑139 (MQ=255)

                                      tgctgTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCa                                                                                                >  1:385320/1‑139 (MQ=255)

                                           ttCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGgcgt                                                                                                                                       <  1:130214/95‑1 (MQ=255)

                                           ttCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGgcgt                                                                                                                                       >  2:130214/1‑95 (MQ=255)

                                                tACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCAt                                                                                      <  2:519176/139‑1 (MQ=255)

                                                  cGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAagag                                                                                                                            >  1:1168461/1‑99 (MQ=255)

                                                  cGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAagag                                                                                                                            <  2:1168461/99‑1 (MQ=255)

                                                     gaggaAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGgcgt                                                                                                                                       <  2:737684/85‑1 (MQ=255)

                                                     gaggaAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGAAGgcgt                                                                                                                                       >  1:737684/1‑85 (MQ=255)

                                                     gaggaAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACAGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCAc                                                                                 >  1:984870/1‑139 (MQ=255)

                                                                ggTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCa                                                                      <  2:156358/139‑1 (MQ=255)

                                                                 gtgtTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGt                                                                                                                         <  2:396805/87‑1 (MQ=255)

                                                                 gtgtTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGt                                                                                                                         >  1:396805/1‑87 (MQ=255)

                                                                   gtTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATg                                                                   >  1:926626/1‑139 (MQ=255)

                                                                       cccGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATAAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGct                                                                                                        <  2:176863/98‑1 (MQ=255)

                                                                       cccGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATAAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGct                                                                                                        >  1:176863/1‑98 (MQ=255)

                                                                            cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGgcgt                                                                                                                                       >  2:1000042/1‑62 (MQ=255)

                                                                            cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTa                                                          >  1:346430/1‑139 (MQ=255)

                                                                                                    gATGGCATCTGAACCTTCGCGGACATGAGTGACGGCgtca                                                                                                                                     <  2:300018/40‑1 (MQ=255)

                                                                                                    gATGGCATCTGAACCTTCGCGGACATGAGTGACGGCgtca                                                                                                                                     >  1:300018/1‑40 (MQ=255)

                                                                                                         cATCTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTcgcg                             >  2:954248/1‑139 (MQ=255)

                                                                                                            cTGAACCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGTGATTTCGCGAAt                          <  2:984870/139‑1 (MQ=255)

                                                                                                               aaCCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCaa                                                                     >  1:401486/1‑93 (MQ=255)

                                                                                                               aaCCTTCGCGGACATGAGTGACGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCaa                                                                     <  2:401486/93‑1 (MQ=255)

                                                                                                                                    cGGCGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCgg  <  2:385320/139‑1 (MQ=255)

GCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGG  >  NC_000913/1971767‑1972037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A