Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,149,963	G→T	G341G (GGC→GGA)	yegI ←	protein kinase‑related putative non‑specific DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,149,963	0	G	T	100.0%	154.9 / NA	49	G341G (GGC→GGA)	yegI	protein kinase‑related putative non‑specific DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/29); total (20/29)

TAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGA  >  NC_000913/2149828‑2150083

tAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCg                                                                                                                       <  2:1038386/139‑1 (MQ=255)

    ggAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCATCTCCGAGAt                                                                                                                   <  2:768059/139‑1 (MQ=255)

         cGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATa                                                                                                              <  2:65568/139‑1 (MQ=255)

           gCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATaaa                                                                                                            <  1:260297/139‑1 (MQ=255)

                 cAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGAt                                                                                                      <  2:688031/139‑1 (MQ=255)

                      ggTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAacac                                                                                                 <  2:1117795/139‑1 (MQ=255)

                         tGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCtt                                                                                              >  2:706405/1‑139 (MQ=255)

                               tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCTGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAATACCTTGATCAt                                                                                        <  1:706405/139‑1 (MQ=255)

                                 aTCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGAGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACAACTTGATTAAcc                                                                                      <  1:706439/139‑1 (MQ=255)

                                  tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCgag                                                                                                                     <  1:1160377/107‑1 (MQ=255)

                                  tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCgag                                                                                                                     >  2:1160377/1‑107 (MQ=255)

                                  tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATaaaa                                                                                                           <  1:332143/117‑1 (MQ=255)

                                  tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATaaaa                                                                                                           >  2:332143/1‑117 (MQ=255)

                                      gTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATaaaa                                                                                                           <  2:539411/113‑1 (MQ=255)

                                      gTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATaaaa                                                                                                           >  1:539411/1‑113 (MQ=255)

                                      gTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcgc                                                                                 <  2:445078/139‑1 (MQ=255)

                                         aTGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGAt                                                                                                      <  2:267764/115‑1 (MQ=255)

                                         aTGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGAt                                                                                                      >  1:267764/1‑115 (MQ=255)

                                           ggCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCAcacc                                                                            >  1:347876/1‑139 (MQ=255)

                                                ttGCAATGGTGGCAGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTcc                                                                                                                        <  1:986859/90‑1 (MQ=255)

                                                ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTcc                                                                                                                        >  2:986859/1‑90 (MQ=255)

                                                 tGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                      <  2:847492/139‑1 (MQ=255)

                                                         tGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCagtcagtc                                                              >  1:834129/1‑139 (MQ=255)

                                                          ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtca                                                                 >  1:236067/1‑135 (MQ=255)

                                                          ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtca                                                                 <  2:236067/135‑1 (MQ=255)

                                                          ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                    <  1:917266/132‑1 (MQ=255)

                                                          ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                    >  2:917266/1‑132 (MQ=255)

                                                           gCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                            <  1:426213/139‑1 (MQ=255)

                                                                           cATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                       >  1:28908/1‑128 (MQ=255)

                                                                           cATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                       <  2:28908/128‑1 (MQ=255)

                                                                            aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCgag                                                                                                                     >  1:134611/1‑65 (MQ=255)

                                                                            aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCgag                                                                                                                     <  2:134611/65‑1 (MQ=255)

                                                                            aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGA‑TATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                          <  1:992558/139‑1 (MQ=255)

                                                                                   tCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTcc                                                                                                                        >  2:293874/1‑55 (MQ=255)

                                                                                   tCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTcc                                                                                                                        <  1:293874/55‑1 (MQ=255)

                                                                                     gCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTg                                  >  1:410746/1‑139 (MQ=255)

                                                                                      cccAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGa                                 >  2:1054081/1‑139 (MQ=255)

                                                                                                cGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                       >  2:606498/1‑139 (MQ=255)

                                                                                                     gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                    >  2:631126/1‑89 (MQ=255)

                                                                                                     gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                    <  1:631126/89‑1 (MQ=255)

                                                                                                       acaAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTa                <  2:98218/139‑1 (MQ=255)

                                                                                                           aaTTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg            <  2:650266/139‑1 (MQ=255)

                                                                                                           aaTTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg            <  2:1063848/139‑1 (MQ=255)

                                                                                                                ccgccgGTGGTAATGACCTCTTCTCCAAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagt                                                                   >  2:631205/1‑79 (MQ=255)

                                                                                                                ccgccgGTGGTAATGACCTCTTCTCCAAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagt                                                                   <  1:631205/79‑1 (MQ=255)

                                                                                                                 cgccgGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                    <  2:1047346/77‑1 (MQ=255)

                                                                                                                 cgccgGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                    >  1:1047346/1‑77 (MQ=255)

                                                                                                                   ccgGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAATCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTa    >  1:43128/1‑139 (MQ=255)

                                                                                                                     ggtggtAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga  <  2:834129/139‑1 (MQ=255)

TAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGA  >  NC_000913/2149828‑2150083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A