Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,149,963	G→T	G341G (GGC→GGA)	yegI ←	protein kinase‑related putative non‑specific DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,149,963	0	G	T	100.0%	90.5 / NA	30	G341G (GGC→GGA)	yegI	protein kinase‑related putative non‑specific DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (12/18); total (12/18)

CTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCC  >  NC_000913/2149827‑2150095

cTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGTTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTcc                                                                                                                                    <  2:484174/139‑1 (MQ=255)

  aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCga                                                                                                                                  >  1:715688/1‑139 (MQ=255)

             ccTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGGGATCAATaaaa                                                                                                                       >  2:522552/1‑139 (MQ=255)

                       ggTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAacac                                                                                                             <  2:226183/139‑1 (MQ=255)

                       ggTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAACAGATAacac                                                                                                             <  2:532715/139‑1 (MQ=255)

                                 gATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                   <  1:49191/139‑1 (MQ=255)

                                           tGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCacac                                                                                         <  2:432772/139‑1 (MQ=255)

                                                 ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAGTGACCTCTTCTCCgag                                                                                                                                 >  1:575593/1‑93 (MQ=255)

                                                 ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCgag                                                                                                                                 <  2:575593/93‑1 (MQ=255)

                                                 ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACggg                                                                                   >  1:324244/1‑139 (MQ=255)

                                                    cAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                <  2:715688/139‑1 (MQ=255)

                                                            gCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                        >  1:271362/1‑139 (MQ=255)

                                                                 gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                   <  2:781567/139‑1 (MQ=255)

                                                                 gCTACAGGCGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCACGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATCATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                   <  1:672976/139‑1 (MQ=255)

                                                                    aCTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCCTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgg                                                                <  1:130545/139‑1 (MQ=255)

                                                                           ccATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAAc                                                         <  2:324244/139‑1 (MQ=255)

                                                                               cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGc                                                     >  2:445/1‑139 (MQ=255)

                                                                               cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGc                                                     <  1:802088/139‑1 (MQ=255)

                                                                                               ttCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                     <  1:719234/139‑1 (MQ=255)

                                                                                                            aaTTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                        <  1:328963/139‑1 (MQ=255)

                                                                                                                  cgccgGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGaaa                                           <  2:2842/114‑1 (MQ=255)

                                                                                                                  cgccgGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGaaa                                           >  1:2842/1‑114 (MQ=255)

                                                                                                                           tAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                      >  1:505605/1‑94 (MQ=255)

                                                                                                                           tAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                      <  2:505605/94‑1 (MQ=255)

                                                                                                                                aCCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGTGTAGAGAGTCCAGTg    >  1:307698/1‑139 (MQ=255)

                                                                                                                                  ctctTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCACTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGcc  >  1:26270/1‑139 (MQ=255)

                                                                                                                                    ctTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                   >  2:191148/1‑72 (MQ=255)

                                                                                                                                    ctTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                   <  1:191148/72‑1 (MQ=255)

                                                                                                                                    ctTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                      <  1:733661/85‑1 (MQ=255)

                                                                                                                                    ctTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                      >  2:733661/1‑85 (MQ=255)

CTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCC  >  NC_000913/2149827‑2150095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A