Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,442,490	+G	coding (727/2007 nt)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,442,486	1	.	G	100.0%	91.7 / NA	27	C241W (TGC→TGG)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/13); total (14/13)

CGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGC‑GGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGT  >  NC_000913/2442348‑2442598

cGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGc                                                                                                                   <  2:611885/139‑1 (MQ=255)

 gCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGC‑g                                                                                                                 <  1:1032501/139‑1 (MQ=255)

 gCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGC‑g                                                                                                                 >  2:695928/1‑139 (MQ=255)

     tGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGC‑ggggg                                                                                                             <  1:695928/139‑2 (MQ=255)

       gCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGC‑gggggtg                                                                                                           <  1:987725/139‑4 (MQ=255)

                 cgcgTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGa                                                                                                  >  2:538028/1‑139 (MQ=255)

                         ggcggcACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCg                                                                                          <  1:1215803/139‑1 (MQ=255)

                              cACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTccc                                                                                     >  1:1148094/1‑139 (MQ=255)

                                   tGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCt                                                                                <  2:443111/139‑1 (MQ=255)

                                    ggCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCg                                                                                          <  1:1133214/128‑1 (MQ=255)

                                    ggCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCg                                                                                          >  2:1133214/1‑128 (MQ=255)

                                        aCATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCcgcg                                                                           >  1:655727/1‑139 (MQ=255)

                                             tACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGc                                                                                                                   >  1:85516/1‑94 (MQ=255)

                                             tACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGc                                                                                                                   <  2:85516/94‑1 (MQ=255)

                                             tACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTg                                                                      >  1:701003/1‑139 (MQ=255)

                                                    gCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAAc                                                               <  2:655727/139‑1 (MQ=255)

                                                        gTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCa                                                           <  1:538028/139‑1 (MQ=255)

                                                            tGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACggg                                                       <  1:975976/139‑1 (MQ=255)

                                                                  ccgcGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGgcagcag                                                 <  2:353062/139‑1 (MQ=255)

                                                                               gACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAgcg                                    <  2:701003/139‑1 (MQ=255)

                                                                                  gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGa                                        >  1:845578/1‑132 (MQ=255)

                                                                                  gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGa                                        <  2:845578/132‑1 (MQ=255)

                                                                                  gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAgcggcg                                 >  2:418425/1‑139 (MQ=255)

                                                                                      gATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTa                             >  2:385068/1‑139 (MQ=255)

                                                                                                         tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACAt                                                                                               >  2:943122/1‑54 (MQ=255)

                                                                                                         tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACAt                                                                                               <  1:943122/54‑1 (MQ=255)

                                                                                                         tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAg          >  1:210212/1‑139 (MQ=255)

                                                                                                           aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCg                                                                                          <  1:157231/57‑1 (MQ=255)

                                                                                                           aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCg                                                                                          >  2:157231/1‑57 (MQ=255)

                                                                                                           aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGTGATTATCGGCggt                     <  1:209873/126‑1 (MQ=255)

                                                                                                           aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCggt                     >  2:209873/1‑126 (MQ=255)

                                                                                                                 ttGGGCGCAAACGGGAAATGCTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCgttgt  >  2:285327/1‑139 (MQ=255)

                                                                                                                                     cTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTa                             >  1:383721/1‑92 (MQ=255)

                                                                                                                                     cTTTGCGGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTa                             <  2:383721/92‑1 (MQ=255)

CGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGC‑GGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGT  >  NC_000913/2442348‑2442598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A