Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,015	G→A	427 (TAG→TAA)	gabT →	4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,015	0	G	A	100.0%	153.5 / NA	48	427 (TAG→TAA)	gabT	4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/22); total (26/22)

TGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGG  >  NC_000913/2793921‑2794146

tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                                         >  1:1064293/1‑139 (MQ=255)

tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                                         >  1:197530/1‑139 (MQ=255)

   tgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTAc                                                                                      >  1:1023234/1‑139 (MQ=255)

    gcgcATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     <  2:197530/139‑1 (MQ=255)

    gcgcATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     <  2:1064293/139‑1 (MQ=255)

         tCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                                                <  2:866805/139‑1 (MQ=255)

               tACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                          <  2:1023234/139‑1 (MQ=255)

                    cTCACCATTGAAGACGCTCATATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  1:717133/1‑139 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                            <  2:508890/116‑1 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                            >  1:508890/1‑116 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  2:954490/1‑139 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  2:805425/1‑139 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  2:51571/1‑139 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  2:1783/1‑139 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  1:660736/1‑139 (MQ=255)

                    cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  1:512950/1‑139 (MQ=255)

                      cACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATaa                                                                   >  1:235742/1‑139 (MQ=255)

                                        gATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATcc                                                 >  2:538137/1‑139 (MQ=255)

                                        gATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATcc                                                 >  2:811302/1‑139 (MQ=255)

                                          tCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                          <  2:1197455/112‑1 (MQ=255)

                                          tCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                          >  1:1197455/1‑112 (MQ=255)

                                               cAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                          <  2:512950/139‑1 (MQ=255)

                                                      tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGt                                   >  2:1178792/1‑139 (MQ=255)

                                                          gaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                               <  2:235742/139‑1 (MQ=255)

                                                            tcatcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAg                             <  2:1078395/139‑1 (MQ=255)

                                                             catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgccgc                                                     >  1:817911/1‑114 (MQ=255)

                                                             catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgccgc                                                     <  2:817911/114‑1 (MQ=255)

                                                              atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAgg                            >  2:1196763/1‑138 (MQ=255)

                                                              atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAgg                            <  1:1196763/138‑1 (MQ=255)

                                                              atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                           >  1:543854/1‑139 (MQ=255)

                                                                  gCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGcc                                                                                                                      >  1:247757/1‑44 (MQ=255)

                                                                  gCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGcc                                                                                                                      <  2:247757/44‑1 (MQ=255)

                                                                   ccAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     >  2:343072/1‑92 (MQ=255)

                                                                   ccAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                     <  1:343072/92‑1 (MQ=255)

                                                                       tgtTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     >  2:454458/1‑72 (MQ=255)

                                                                       tgtTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     <  1:454458/72‑1 (MQ=255)

                                                                          tttGATGGTGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCCTCTTGTCCGGTCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAGAAGGAAGAAATCTATGt               <  1:998810/139‑1 (MQ=39)

                                                                             gatgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTgg            <  2:660736/139‑1 (MQ=255)

                                                                                 aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTgc                                                                                                        <  2:367387/43‑1 (MQ=255)

                                                                                 aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTgc                                                                                                        >  1:367387/1‑43 (MQ=255)

                                                                                 aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     <  1:655218/62‑1 (MQ=255)

                                                                                 aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     >  2:655218/1‑62 (MQ=255)

                                                                                 aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAAt                                           <  1:567232/104‑1 (MQ=255)

                                                                                 aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAAt                                           >  2:567232/1‑104 (MQ=255)

                                                                                   gCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     <  2:734750/60‑1 (MQ=255)

                                                                                   gCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                     >  1:734750/1‑60 (MQ=255)

                                                                                    cGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTa     <  2:1076022/139‑1 (MQ=255)

                                                                                       aGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAgg  <  1:538137/139‑1 (MQ=255)

                                                                                       aGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAgg  <  1:811302/139‑1 (MQ=255)

TGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGG  >  NC_000913/2793921‑2794146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A