Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,015	G→A	427 (TAG→TAA)	gabT →	4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,015	0	G	A	100.0%	164.4 / NA	51	427 (TAG→TAA)	gabT	4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/22); total (29/22)

CGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGG  >  NC_000913/2793909‑2794141

cGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGcgcg                                                                                                >  1:294627/1‑139 (MQ=255)

            tgctgcGCATGCTTGTACCGCCCACCATTGCAGCCGCTCAGATCCGTCAGGGCCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                                    <  2:486246/139‑1 (MQ=255)

            tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                                    >  1:724750/1‑139 (MQ=255)

               tgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTAc                                                                                 >  1:554473/1‑139 (MQ=255)

                gcgcATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                <  2:294627/139‑1 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAgg                                                                                                            <  2:563782/95‑1 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAgg                                                                                                            >  1:563782/1‑95 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTg                                                                                                    <  1:1021414/103‑1 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTg                                                                                                    >  2:1021414/1‑103 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                >  2:984450/1‑123 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                <  1:984450/123‑1 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:907991/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:583426/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  1:849143/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  1:1037404/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:476416/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:461893/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:27816/1‑139 (MQ=255)

                                cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:265803/1‑139 (MQ=255)

                                       ttGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGt                                                         >  1:732104/1‑139 (MQ=255)

                                                      tCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                          <  1:749189/139‑1 (MQ=255)

                                                        cGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACa                                        <  1:461893/139‑1 (MQ=255)

                                                        cGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACa                                        <  2:554473/139‑1 (MQ=255)

                                                           cAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                     <  1:1014776/139‑1 (MQ=255)

                                                             gggTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTg                                   <  1:619637/139‑1 (MQ=255)

                                                               gTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                <  1:350811/92‑1 (MQ=255)

                                                               gTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                >  2:350811/1‑92 (MQ=255)

                                                               gTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTa                                 >  1:177562/1‑139 (MQ=255)

                                                               gTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTa                                 >  2:626092/1‑139 (MQ=255)

                                                               gTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTa                                 >  1:1052312/1‑139 (MQ=255)

                                                               gTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTa                                 >  2:971146/1‑139 (MQ=255)

                                                                      gaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                          <  2:724750/139‑1 (MQ=255)

                                                                         catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAAt                                      >  2:664540/1‑124 (MQ=255)

                                                                         catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAAt                                      <  1:664540/124‑1 (MQ=255)

                                                                         catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAgg                       <  1:972242/139‑1 (MQ=255)

                                                                          atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                      >  2:906388/1‑139 (MQ=255)

                                                                          atcaACCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTATCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                      >  2:614822/1‑139 (MQ=255)

                                                                           tcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                     <  2:849143/139‑1 (MQ=255)

                                                                               ccAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGaaa                 <  1:626092/139‑1 (MQ=255)

                                                                                 aGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAgg                       >  1:975105/1‑131 (MQ=255)

                                                                                 aGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAgg                       <  2:975105/131‑1 (MQ=255)

                                                                                         gatgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTg                                                                                                    <  2:427430/46‑1 (MQ=255)

                                                                                         gatgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTg                                                                                                    >  1:427430/1‑46 (MQ=255)

                                                                                         gatgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTgg       <  1:614822/139‑1 (MQ=255)

                                                                                          atgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACACTCTGTACGTGAACAGGAAGAAATCTATGTTGGc      >  2:139420/1‑139 (MQ=255)

                                                                                          atgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGc      <  1:907991/139‑1 (MQ=255)

                                                                                             aGGCGAAGCAGTAACGCCGCTCCTATGACGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCgg   >  2:630322/1‑139 (MQ=255)

                                                                                              ggCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCggg  <  2:774947/139‑1 (MQ=255)

                                                                                              ggCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCggg  >  2:152013/1‑139 (MQ=255)

                                                                                               gCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATcc                                            >  2:908206/1‑96 (MQ=255)

                                                                                               gCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATcc                                            <  1:908206/96‑1 (MQ=255)

CGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGG  >  NC_000913/2793909‑2794141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A