Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,188,124	A→G	E925E (GAA→GAG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,188,124	0	A	G	100.0%	237.8 / NA	65	E925E (GAA→GAG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/33); total (32/33)

AGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAG  >  NC_000913/4187992‑4188258

aGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGt                                                                                                                                  <  2:82075/139‑1 (MQ=255)

 gTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 <  2:610725/139‑1 (MQ=255)

      ttCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAg                                                                                                                            <  2:1216928/139‑1 (MQ=255)

      ttCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAg                                                                                                                            >  2:1082370/1‑139 (MQ=255)

      ttCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAg                                                                                                                            >  2:1050358/1‑139 (MQ=255)

                  ttGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGt                                                                                                                <  1:389425/139‑1 (MQ=255)

                     tgACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACg                                                                                                             >  2:109056/1‑139 (MQ=255)

                      gACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAGCAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGCGCCGGGTACACAGCTGACCATGCGTACGt                                                                                                            <  2:814434/139‑1 (MQ=255)

                          ccGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCca                                                                                                        >  2:145604/1‑139 (MQ=255)

                           cGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCcac                                                                                                       >  2:531133/1‑139 (MQ=255)

                             aCTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACAt                                                                                                     <  1:1050358/139‑1 (MQ=255)

                                  ggTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCggtg                                                                                                <  1:1082370/139‑1 (MQ=255)

                                        tgtgCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTc                                                                                                          >  2:665097/1‑123 (MQ=255)

                                        tgtgCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTc                                                                                                          <  1:665097/123‑1 (MQ=255)

                                             gcACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCAtct                                                                                     >  2:953482/1‑139 (MQ=255)

                                             gcACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCAtct                                                                                     >  2:27806/1‑139 (MQ=255)

                                                 tGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTg                                                                                 <  1:953482/139‑1 (MQ=255)

                                                 tGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTg                                                                                 <  1:145604/139‑1 (MQ=255)

                                                  gCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGc                                                                                >  1:512143/1‑139 (MQ=255)

                                                   cTACGGTCGTGACCTGGCGCGTTGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCg                                                                               >  2:270897/1‑139 (MQ=255)

                                                   cTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCg                                                                               >  2:167745/1‑139 (MQ=255)

                                                           gtggccTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACg                                                                                                             >  2:366890/5‑101 (MQ=255)

                                                           gTGGCCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACg                                                                                                             <  1:366890/101‑1 (MQ=255)

                                                           gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCt                                                                                                                          <  1:522040/88‑1 (MQ=255)

                                                           gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCt                                                                                                                          >  2:522040/1‑88 (MQ=255)

                                                           gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGa                                                                       >  2:1146732/1‑139 (MQ=255)

                                                           gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGa                                                                       >  2:1001759/1‑139 (MQ=255)

                                                             gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGATCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAAt                                                                     >  2:773846/1‑139 (MQ=255)

                                                             gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAAt                                                                     >  1:407347/1‑139 (MQ=255)

                                                              aCCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATc                                                                    <  2:949908/139‑1 (MQ=255)

                                                                   gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATc                                                                    >  2:403194/1‑134 (MQ=255)

                                                                   gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATc                                                                    <  1:403194/134‑1 (MQ=255)

                                                                        tGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCgg                                                                                                  <  1:124689/99‑1 (MQ=255)

                                                                        tGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCgg                                                                                                  >  2:124689/1‑99 (MQ=255)

                                                                        tGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCaa                                                          <  1:1001759/139‑1 (MQ=255)

                                                                        tGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCaa                                                          <  1:1146732/139‑1 (MQ=255)

                                                                              catcatCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCgg                                                                              >  1:1073458/1‑113 (MQ=255)

                                                                              catcatCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCgg                                                                              <  2:1073458/113‑1 (MQ=255)

                                                                                  atcaACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACa                                                <  2:147669/139‑1 (MQ=255)

                                                                                   tcaACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACaa                                               >  2:204292/1‑139 (MQ=255)

                                                                                          gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGcc                                                                                                                                      >  1:1206435/1‑45 (MQ=255)

                                                                                          gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTg                                                                                 >  1:391433/1‑98 (MQ=255)

                                                                                          gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTg                                                                                 <  2:391433/98‑1 (MQ=255)

                                                                                           ggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGTGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCa                                       >  2:358351/1‑139 (MQ=255)

                                                                                              gAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGa                                                                                                                        <  1:1140174/55‑1 (MQ=255)

                                                                                              gAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGa                                                                                                                        >  2:1140174/1‑55 (MQ=255)

                                                                                               aaGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 <  1:340225/45‑1 (MQ=255)

                                                                                               aaGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 >  2:340225/1‑45 (MQ=255)

                                                                                               aaGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAgg                                            <  2:280954/130‑1 (MQ=255)

                                                                                               aaGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAgg                                            >  1:280954/1‑130 (MQ=255)

                                                                                                aGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAg                                  <  1:167745/139‑1 (MQ=255)

                                                                                                     tCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAg                             >  2:31247/1‑139 (MQ=255)

                                                                                                     tCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAg                             >  1:116209/1‑139 (MQ=255)

                                                                                                       ggTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCa                           <  2:1111073/139‑1 (MQ=255)

                                                                                                              tCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGaa                    <  1:27806/139‑1 (MQ=255)

                                                                                                              tCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGaa                    <  2:407347/139‑1 (MQ=255)

                                                                                                                   gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCa                                                                                                                     >  2:668339/1‑37 (MQ=255)

                                                                                                                   gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCa                                                                                                                     <  1:668339/37‑1 (MQ=255)

                                                                                                                   gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCa                                       <  1:997195/115‑1 (MQ=255)

                                                                                                                   gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCa                                       >  2:997195/1‑115 (MQ=255)

                                                                                                                       aGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCgg                                                                              >  1:1073957/1‑72 (MQ=255)

                                                                                                                       aGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCgg                                                                              <  2:1073957/72‑1 (MQ=255)

                                                                                                                             tCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTc     <  2:647297/139‑1 (MQ=255)

                                                                                                                                gTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGTGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAg  <  1:358351/139‑1 (MQ=255)

                                                                                                                                gTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAg  <  2:16916/139‑1 (MQ=255)

AGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAG  >  NC_000913/4187992‑4188258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A