Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,189,084 |
C→T |
G1245G (GGC→GGT) |
rpoC → |
RNA polymerase, beta prime subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,189,084 | 0 | C | T | 100.0%
| 99.9
/ NA
| 32 | G1245G (GGC→GGT) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/14); total (18/14) |
AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAA > NC_000913/4188963‑4189215
|
aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATaa < 2:1175750/139‑1 (MQ=255)
ccGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgt < 2:429951/139‑1 (MQ=255)
ggAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtg < 1:942619/139‑1 (MQ=255)
ccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa < 2:74711/97‑1 (MQ=255)
ccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa > 1:74711/1‑97 (MQ=255)
ccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTa > 2:1048325/1‑139 (MQ=255)
tctgcgtctgcgTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATc > 2:935296/1‑139 (MQ=255)
cgtctgcgtGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgt > 2:1051728/1‑107 (MQ=255)
cgtctgcgtGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgt < 1:1051728/107‑1 (MQ=255)
ctgcgtGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcg > 2:728900/1‑139 (MQ=255)
tGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa < 2:93198/62‑1 (MQ=255)
tGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa > 1:93198/1‑62 (MQ=255)
gTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACt < 1:894042/136‑1 (MQ=255)
gTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACt > 2:894042/1‑136 (MQ=255)
gTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAgg < 1:728900/139‑1 (MQ=255)
gTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAgg < 1:1266586/139‑1 (MQ=255)
ttAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGt > 2:240873/1‑139 (MQ=255)
gAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGAt > 1:1308063/1‑38 (MQ=255)
gAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGAt < 2:1308063/38‑1 (MQ=255)
gTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGGTGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATAct > 1:174847/1‑139 (MQ=255)
aCAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATActct < 1:1048325/139‑1 (MQ=255)
cAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACtctc > 2:1332039/1‑139 (MQ=255)
gTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCa > 2:1370529/1‑139 (MQ=255)
gTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt > 2:490171/1‑77 (MQ=255)
gTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt < 1:490171/77‑1 (MQ=255)
tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgttc < 2:1365484/47‑1 (MQ=255)
tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgttc > 1:1365484/1‑47 (MQ=255)
tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGTTGCGTa < 2:65083/62‑1 (MQ=255)
tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGTTGCGTa > 1:65083/1‑62 (MQ=255)
tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGAGTCAAGATCGCa > 1:570296/1‑139 (MQ=255)
cTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCaa > 2:249659/1‑139 (MQ=255)
tGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCaaa > 2:127288/1‑139 (MQ=255)
|
AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAA > NC_000913/4188963‑4189215
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
