Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,490,141 |
C→T |
*333* (TAG→TAA) |
idnR ← |
transcriptional repressor, 5‑gluconate‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,490,141 | 0 | C | T | 100.0%
| 135.9
/ NA
| 43 | *333* (TAG→TAA) | idnR | transcriptional repressor, 5‑gluconate‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/22); total (21/22) |
CGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTG > NC_000913/4490007‑4490273
|
ttagccattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa > 2:1161919‑M2/121‑139 (MQ=255)
ttagccattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa > 1:748779‑M2/121‑139 (MQ=255)
cattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg < 1:1122764‑M2/24‑1 (MQ=255)
tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg < 2:748779‑M2/27‑1 (MQ=255)
cccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc < 1:1161919‑M2/28‑1 (MQ=255)
cagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGa < 2:1008201‑M2/33‑1 (MQ=255)
agtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGAt < 1:136833‑M2/34‑1 (MQ=255)
cagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGa < 1:1157538‑M2/42‑1 (MQ=255)
tctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc > 1:738953‑M2/94‑121 (MQ=255)
tctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc < 2:738953‑M2/28‑1 (MQ=255)
tgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg < 1:659798‑M2/27‑1 (MQ=255)
tgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg > 2:659798‑M2/90‑116 (MQ=255)
ggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATc > 2:1184992‑M2/66‑139 (MQ=255)
cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg < 1:666837‑M2/63‑1 (MQ=255)
cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg > 2:666837‑M2/60‑122 (MQ=255)
gagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAAtt > 1:600218‑M2/53‑139 (MQ=255)
atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggt < 1:663589‑M2/67‑1 (MQ=255)
atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggt > 2:663589‑M2/48‑114 (MQ=255)
tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttgt < 1:965027‑M2/64‑1 (MQ=255)
tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttgt > 2:965027‑M2/47‑110 (MQ=255)
agtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAg < 1:76251‑M2/95‑1 (MQ=255)
agtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAg > 2:76251‑M2/41‑135 (MQ=255)
gtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACa > 1:474890‑M2/40‑139 (MQ=255)
agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa > 2:880040‑M2/27‑61 (MQ=255)
agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa < 1:880040‑M2/35‑1 (MQ=255)
agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGc > 2:236240‑M2/27‑122 (MQ=255)
agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGc < 1:236240‑M2/96‑1 (MQ=255)
agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt > 2:1012481‑M2/27‑139 (MQ=255)
gtataggcaacgtccggcaaagataGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc < 1:1184992‑M2/114‑1 (MQ=255)
acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc > 2:987838‑M2/17‑63 (MQ=255)
acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc < 1:987838‑M2/47‑1 (MQ=255)
cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg < 2:1215705‑M2/50‑1 (MQ=255)
cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg > 1:1215705‑M2/16‑65 (MQ=255)
ggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa > 2:490824‑M2/11‑65 (MQ=255)
ggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa < 1:490824‑M2/55‑1 (MQ=255)
caaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACg < 2:474890‑M2/131‑1 (MQ=255)
aagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACggg < 2:401992‑M2/133‑1 (MQ=255)
aaaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAAc < 2:1028203/50‑1 (MQ=255)
aaaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAAc > 1:1028203/1‑50 (MQ=255)
aattAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCt > 2:1072531/4‑139 (MQ=255)
aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACg > 1:983120/1‑137 (MQ=255)
aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACg < 2:983120/137‑1 (MQ=255)
aaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTg > 1:930989/1‑139 (MQ=255)
|
CGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTG > NC_000913/4490007‑4490273
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
