Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,375	C→T	A255A (GCG→GCA)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,375	0	C	T	100.0%	175.1 / NA	54	A255A (GCG→GCA)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/31); total (23/31)

ATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGG  >  NC_000913/4490247‑4490505

atatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCg                                                                                                                          >  2:312061/1‑139 (MQ=255)

atatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCg                                                                                                                          <  2:56999/139‑1 (MQ=255)

atatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCg                                                                                                                          <  1:278182/139‑1 (MQ=255)

  atCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCa                                                                                                                        >  2:87582/1‑139 (MQ=255)

   tCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCaa                                                                                                                       >  2:32725/1‑139 (MQ=255)

      aaaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAatat                                                                                                                    >  2:101251/1‑139 (MQ=255)

      aaaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAatat                                                                                                                    >  1:537925/1‑139 (MQ=255)

           gggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                                                 >  1:757492/1‑121 (MQ=255)

           gggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                                                 <  2:757492/121‑1 (MQ=255)

            ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  1:144458/1‑139 (MQ=255)

            ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  1:439235/1‑139 (MQ=255)

            ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  1:435173/1‑139 (MQ=255)

            ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  1:262487/1‑139 (MQ=255)

            ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              <  1:219054/139‑1 (MQ=255)

              aGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTg                                                                                                            <  2:144458/139‑1 (MQ=255)

                tAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  2:170817/139‑1 (MQ=255)

                 aaTTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTg                                                                                                         <  2:720430/139‑1 (MQ=255)

                          ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGGAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  2:522254/139‑1 (MQ=255)

                          ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  1:537598/139‑1 (MQ=255)

                           gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATacac                                                                                               >  1:303794/1‑139 (MQ=255)

                           gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATacac                                                                                               >  2:526679/1‑139 (MQ=255)

                                     ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                                                     >  2:614552/1‑139 (MQ=255)

                                     ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                                                     >  2:123339/1‑139 (MQ=255)

                                          aTTTGCCTGCCAATCTCCTGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGt                                                                                <  2:537925/139‑1 (MQ=255)

                                          aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGt                                                                                >  2:796539/1‑139 (MQ=255)

                                          aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGt                                                                                <  2:799982/139‑1 (MQ=255)

                                                    cAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAg                                                                      >  2:597686/1‑139 (MQ=255)

                                                         tcCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 <  1:196652/139‑1 (MQ=255)

                                                         tcCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 <  1:704928/139‑1 (MQ=255)

                                                         tcCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 <  1:32725/139‑1 (MQ=255)

                                                         tcCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 <  1:388670/139‑1 (MQ=255)

                                                             gCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 <  1:250764/135‑1 (MQ=255)

                                                             gCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 >  2:250764/1‑135 (MQ=255)

                                                             gCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACg                                                             >  2:792799/1‑139 (MQ=255)

                                                                  tGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          >  2:97394/1‑89 (MQ=255)

                                                                  tGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  1:97394/89‑1 (MQ=255)

                                                                  tGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTa                                                        >  2:278457/1‑139 (MQ=255)

                                                                     aaaCCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACt                                                     <  2:303794/139‑1 (MQ=255)

                                                                       aCCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGc                                                   <  1:614552/139‑1 (MQ=255)

                                                                         ccGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt                                                 <  1:278457/139‑1 (MQ=255)

                                                                         ccGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt                                                 <  2:413426/139‑1 (MQ=255)

                                                                             aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          >  1:742290/1‑78 (MQ=255)

                                                                             aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  2:742290/78‑1 (MQ=255)

                                                                                           cTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCa                                                                                                                        <  2:40131/50‑1 (MQ=255)

                                                                                           cTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCa                                                                                                                        >  1:40131/1‑50 (MQ=255)

                                                                                                      aGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTg                    <  1:383734/139‑1 (MQ=255)

                                                                                                        gTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGa                  >  2:111595/1‑139 (MQ=255)

                                                                                                           tcgctcgcGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTc               <  2:439235/139‑1 (MQ=255)

                                                                                                           tcgctcgcGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTc               <  2:435173/139‑1 (MQ=255)

                                                                                                           tcgctcgcGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTc               <  1:477816/139‑1 (MQ=255)

                                                                                                             gctcgcGGAACAGCAGAAGTGCACCCATCGAAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAATGGCATCACGCATTATCTGCATTCCTAAATGCATGGATGAGATGGCGCGTGGATTCAt             <  1:796539/139‑1 (MQ=255)

                                                                                                                 gcgGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGt         <  1:312061/139‑1 (MQ=255)

                                                                                                                  cgGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTa        <  1:792799/139‑1 (MQ=255)

                                                                                                                        agcagAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATgggg  <  1:111595/139‑1 (MQ=255)

ATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGG  >  NC_000913/4490247‑4490505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A