Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,375	C→T	A255A (GCG→GCA)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,375	0	C	T	100.0%	149.9 / NA	47	A255A (GCG→GCA)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/28); total (19/28)

CCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGG  >  NC_000913/4490240‑4490505

ccGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                                                 <  2:572282/139‑1 (MQ=255)

  gACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               <  2:406702/139‑1 (MQ=255)

                cGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               >  2:320274/1‑125 (MQ=255)

                cGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               <  1:320274/125‑1 (MQ=255)

                   ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              <  2:999177/139‑1 (MQ=255)

                   ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  2:890725/1‑139 (MQ=255)

                   ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  2:415618/1‑139 (MQ=255)

                   ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGATCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  2:717425/1‑139 (MQ=255)

                       tAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  1:296580/139‑1 (MQ=255)

                       tAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  2:53284/139‑1 (MQ=255)

                          ttACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCa                                                                                                       >  1:96456/1‑139 (MQ=255)

                                 ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               <  2:701653/108‑1 (MQ=255)

                                 ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               >  1:701653/1‑108 (MQ=255)

                                 ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  2:96456/139‑1 (MQ=255)

                                 ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  2:213058/139‑1 (MQ=255)

                                 ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  1:717425/139‑1 (MQ=255)

                                   cGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                                                 >  2:910443/1‑104 (MQ=255)

                                   cGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                                                 <  1:910443/104‑1 (MQ=255)

                                       gCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCt                                                                                          <  1:250963/139‑1 (MQ=255)

                                                      cctgccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          >  2:1090548/1‑108 (MQ=255)

                                                      cctgccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  1:1090548/108‑1 (MQ=255)

                                                      cctgccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgcg                                                                           >  1:813486/1‑139 (MQ=255)

                                                          ccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTaa                                                                       >  2:601746/1‑139 (MQ=255)

                                                          ccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTaa                                                                       >  2:558212/1‑139 (MQ=255)

                                                                tcCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                                                 <  2:1128962/139‑1 (MQ=255)

                                                                  cAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCa                                                               <  1:558212/139‑1 (MQ=255)

                                                                  cAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCa                                                               <  1:555322/139‑1 (MQ=255)

                                                                  cAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCa                                                               <  2:477790/139‑1 (MQ=255)

                                                                          gAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTaa                                                       >  1:877640/1‑139 (MQ=255)

                                                                          gAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTaa                                                       >  1:1113399/1‑139 (MQ=255)

                                                                               cccGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  2:37955/1‑79 (MQ=255)

                                                                               cccGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              <  1:37955/79‑1 (MQ=255)

                                                                                    aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt                                             <  2:1114118/139‑1 (MQ=255)

                                                                                    aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt                                             <  2:877640/139‑1 (MQ=255)

                                                                                        gAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaat                                         <  1:415618/139‑1 (MQ=255)

                                                                                          aaTTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATACCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatga                                       <  1:34197/139‑1 (MQ=255)

                                                                                                          gCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgcgc                                                                          >  2:211986/1‑88 (MQ=255)

                                                                                                          gCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgcgc                                                                          <  1:211986/88‑1 (MQ=255)

                                                                                                          gCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGc                                                   <  2:1097968/111‑1 (MQ=255)

                                                                                                          gCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGc                                                   >  1:1097968/1‑111 (MQ=255)

                                                                                                                         cgGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  2:288816/41‑1 (MQ=255)

                                                                                                                         cgGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          >  1:288816/1‑41 (MQ=255)

                                                                                                                         cgGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCa                                                               >  2:240122/1‑84 (MQ=255)

                                                                                                                         cgGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCa                                                               <  1:240122/84‑1 (MQ=255)

                                                                                                                          ggCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTaa       >  2:734876/1‑139 (MQ=255)

                                                                                                                          ggCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACACATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTaa       <  1:650285/139‑1 (MQ=255)

                                                                                                                               agcagAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATgggg  <  1:43498/139‑1 (MQ=255)

CCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGG  >  NC_000913/4490240‑4490505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A