Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,493,416 |
C→T |
G331S (GGT→AGT) |
idnD ← |
L‑idonate 5‑dehydrogenase, NAD‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,493,416 | 0 | C | T | 100.0%
| 139.1
/ NA
| 44 | G331S (GGT→AGT) | idnD | L‑idonate 5‑dehydrogenase, NAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/20); total (24/20) |
CCTGTGCTGAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGA > NC_000913/4493294‑4493550
|
ccTGTGCTGAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc < 1:744698/139‑1 (MQ=255)
gAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGACACGTAGCGCCTCTTCCAg < 1:445370/139‑1 (MQ=255)
aaCCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAgg > 2:555856/1‑139 (MQ=255)
gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt > 1:325593/1‑135 (MQ=255)
gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt < 2:325593/135‑1 (MQ=255)
gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGt > 2:245665/1‑139 (MQ=255)
gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGt > 2:180769/1‑139 (MQ=255)
gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGt > 1:343228/1‑139 (MQ=255)
tAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTg < 1:245665/139‑1 (MQ=255)
atTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGAATTATTCCTTTATTTAGCTCTTAGAAAACAAGCTTGACTTTGTCTGCCTGGATTTTATCACTGGCGAAGCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATAtt < 1:37508/139‑1 (MQ=255)
ttCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc > 2:602984/1‑114 (MQ=255)
ttCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc < 1:602984/114‑1 (MQ=255)
tCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc < 2:343228/139‑1 (MQ=255)
tCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATa > 2:319753/1‑139 (MQ=255)
tGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc < 2:747706/112‑1 (MQ=255)
tGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc > 1:747706/1‑112 (MQ=255)
tattatTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc > 2:382289/1‑79 (MQ=255)
tattatTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc < 1:382289/79‑1 (MQ=255)
tattCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTc > 1:60309/1‑139 (MQ=255)
ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCa < 1:390550/104‑1 (MQ=255)
ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCa > 2:390550/1‑104 (MQ=255)
ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgc > 1:745172/1‑139 (MQ=255)
ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCGGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgc > 1:569013/1‑139 (MQ=255)
tCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCGCTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgcc < 1:221792/139‑1 (MQ=255)
tCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc > 2:112958/1‑104 (MQ=255)
tCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc < 1:112958/104‑1 (MQ=255)
cTTTATTTATCTCTTAGAACACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt < 2:227216/81‑1 (MQ=255)
cTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt > 1:227216/1‑81 (MQ=255)
cTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccag > 1:668055/1‑139 (MQ=255)
ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc < 1:717597/69‑1 (MQ=255)
ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc > 2:717597/1‑69 (MQ=255)
ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc > 2:119621/1‑139 (MQ=255)
ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc > 1:311557/1‑139 (MQ=255)
atttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCAt < 2:668055/139‑1 (MQ=255)
ctctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt > 1:14920/1‑139 (MQ=255)
ctctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt > 1:324/1‑139 (MQ=255)
tctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTg < 2:14920/139‑1 (MQ=255)
ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc < 2:150097/63‑1 (MQ=255)
ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc > 1:150097/1‑63 (MQ=255)
tgctgcCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTaaaa > 1:791580/1‑139 (MQ=255)
gcCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCTGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGa < 1:180769/139‑1 (MQ=255)
ggTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAg > 1:780720/1‑72 (MQ=255)
ggTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAg < 2:780720/72‑1 (MQ=255)
tCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGa < 2:60309/139‑1 (MQ=255)
|
CCTGTGCTGAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGA > NC_000913/4493294‑4493550
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
