Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,495,332	A→G	L293P (CTG→CCG)	ahr ←	broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,495,332	0	A	G	100.0%	178.8 / NA	50	L293P (CTG→CCG)	ahr	broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/25); total (25/25)

GCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGCCTCAAAA  >  NC_000913/4495214‑4495466

gcgGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTa                                                                                                                    >  2:1106832/1‑139 (MQ=255)

   gTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCa                                                                                                                 <  1:1030356/139‑1 (MQ=255)

              tACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGAc                                                                                                      >  2:1468527/1‑139 (MQ=255)

               aCCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACg                                                                                                     <  1:645586/139‑1 (MQ=255)

                      cgcACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGAt                                                                                              <  1:1193265/139‑1 (MQ=255)

                                   tGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTaa                                                                                 >  2:641592/1‑139 (MQ=255)

                                     gCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAg                                                                                                                   >  2:1659493/1‑103 (MQ=255)

                                     gCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAg                                                                                                                   <  1:1659493/103‑1 (MQ=255)

                                     gCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACg                                                                               >  1:1579933/1‑139 (MQ=255)

                                       gtcgtTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGcc                                                                                          >  2:1339990/1‑126 (MQ=255)

                                       gtcgtTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGcc                                                                                          <  1:1339990/126‑1 (MQ=255)

                                           ttAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg                                                                         <  1:875238/139‑1 (MQ=255)

                                           ttAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg                                                                         <  1:395202/139‑1 (MQ=255)

                                           ttAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg                                                                         <  1:1032384/139‑1 (MQ=255)

                                                  tCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAac                                                                  >  2:378451/1‑139 (MQ=255)

                                                                  ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGc                                                                                       <  2:1800784/102‑1 (MQ=255)

                                                                  ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGc                                                                                       >  1:1800784/1‑102 (MQ=255)

                                                                  ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTgaga                                                  >  2:280556/1‑139 (MQ=255)

                                                                  ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTgaga                                                  >  1:935129/1‑139 (MQ=255)

                                                                    cGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTa                                                                             <  2:1799759/110‑1 (MQ=255)

                                                                    cGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTa                                                                             >  1:1799759/1‑110 (MQ=255)

                                                                    cGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAAc                                                >  2:214454/1‑139 (MQ=255)

                                                                    cGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAAc                                                <  1:641592/139‑1 (MQ=255)

                                                                       tggtCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGc                                             >  1:766084/1‑139 (MQ=255)

                                                                          tCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCacc                                          <  1:1468527/139‑1 (MQ=255)

                                                                            ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGa                                                                    <  1:1525922/111‑1 (MQ=255)

                                                                            ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGa                                                                    >  2:1525922/1‑111 (MQ=255)

                                                                            ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga                                        <  1:1628837/139‑1 (MQ=255)

                                                                            ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga                                        >  2:1724188/1‑139 (MQ=255)

                                                                            ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga                                        >  2:1683381/1‑139 (MQ=255)

                                                                                      tgctgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGaa                              <  2:935129/139‑1 (MQ=255)

                                                                                       gctgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg                                                                         >  1:270041/1‑95 (MQ=255)

                                                                                       gctgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg                                                                         <  2:270041/95‑1 (MQ=255)

                                                                                        ctgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACg                                                                                                     <  2:1178367/66‑1 (MQ=255)

                                                                                        ctgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAAt                            >  1:796125/1‑139 (MQ=255)

                                                                                        ctgaGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACg                                                                                                     >  1:1178367/1‑66 (MQ=255)

                                                                                         tgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAAtt                           <  1:214454/139‑1 (MQ=255)

                                                                                           cgggcggCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTAc                         <  1:280556/139‑1 (MQ=255)

                                                                                             ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTaaa                                                                           <  1:1034/87‑1 (MQ=255)

                                                                                             ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTaaa                                                                           >  2:1034/1‑87 (MQ=255)

                                                                                                ggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAgcggc                                                        <  2:1485061/103‑1 (MQ=255)

                                                                                                ggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAgcggc                                                        >  1:1485061/1‑103 (MQ=255)

                                                                                                  cAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCa                                                                                                                 <  2:195986/44‑1 (MQ=255)

                                                                                                  cAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCa                                                                                                                 >  1:195986/1‑44 (MQ=255)

                                                                                                  cAAAACACATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATa                  >  1:1799005/1‑139 (MQ=255)

                                                                                                        gCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAg            <  2:796125/139‑1 (MQ=255)

                                                                                                        gCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAg            <  2:1579933/139‑1 (MQ=255)

                                                                                                             aGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGCCt       <  1:1683381/139‑1 (MQ=255)

                                                                                                                 tACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGCCTCaaa   >  1:758507/1‑139 (MQ=255)

                                                                                                                  aCGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCACCTCaaaa  >  1:647243/1‑139 (MQ=255)

GCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGCCTCAAAA  >  NC_000913/4495214‑4495466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A