Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,495,332 |
A→G |
L293P (CTG→CCG) |
ahr ← |
broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,495,332 | 0 | A | G | 97.4%
| 135.0
/ NA
| 39 | L293P (CTG→CCG) | ahr | broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (17/21); minor base T (1/0); total (18/21) |
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGCC > NC_000913/4495214‑4495460
|
gcgGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTa < 2:1278182/139‑1 (MQ=255)
cgGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAg < 2:638528/139‑1 (MQ=255)
ggTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGc > 1:1006566/1‑128 (MQ=255)
ggTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGc < 2:1006566/128‑1 (MQ=255)
gTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCa < 2:1070311/139‑1 (MQ=255)
gcCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCag > 1:1101537/1‑139 (MQ=255)
gcCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCag > 2:331022/1‑139 (MQ=255)
ccTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCaga < 2:1101537/139‑1 (MQ=255)
ccTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCaga < 2:1224020/139‑1 (MQ=255)
cgcACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGAt > 2:615448/1‑139 (MQ=255)
cacaTGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCg > 2:206904/1‑139 (MQ=255)
aTGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGTCGGTAGCAGAACCAGAGACGCTGCGATCGccc > 1:522107/1‑139 (MQ=255)
tGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCaa < 1:331022/139‑1 (MQ=255)
gCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACg > 1:1191906/1‑139 (MQ=255)
gtcgtTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTa > 2:184969/1‑139 (MQ=255)
cgtTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGGCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTaaa > 2:958266/1‑139 (MQ=255)
tAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGc < 1:615448/139‑1 (MQ=255)
ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAg < 2:873085/74‑1 (MQ=255)
ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAg > 1:873085/1‑74 (MQ=255)
ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg > 2:1146247/1‑116 (MQ=255)
ttCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAgg < 1:1146247/116‑1 (MQ=255)
tCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCacc < 2:634198/139‑1 (MQ=255)
tCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCacc > 1:1309443/1‑139 (MQ=255)
ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGTCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga < 2:522107/139‑1 (MQ=255)
ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGAt < 1:657265/85‑1 (MQ=255)
ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGAt > 2:657265/1‑85 (MQ=255)
ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGCCTCATAAGGCGTGCCGGTAGCAGAACCAGAGTCGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga < 1:889881/139‑1 (MQ=255)
aaCCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTa < 1:206904/139‑1 (MQ=255)
tgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGGCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAAtt < 1:958266/139‑1 (MQ=255)
tgcGGGCGGCAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga < 2:794256/126‑1 (MQ=255)
tgagagcggcAAAACGCATCAGCTTACGCTGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCaccga > 1:794256/6‑126 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGc < 2:1132388/48‑1 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGc > 1:1132388/1‑48 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGcc > 1:310189/1‑91 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGcc < 2:310189/91‑1 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTAc < 2:654727/137‑1 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTAc > 1:654727/1‑137 (MQ=255)
ggcggcAAAACGCATCAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCg > 2:1331629/1‑139 (MQ=255)
cAGCTTACGCGGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGcc < 2:1309443/139‑1 (MQ=255)
|
GCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTACCGCCATAGGTCAGCGCC > NC_000913/4495214‑4495460
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
