Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,565,966 C→T *427* (TAG→TAA)  yjiN ← zinc‑type alcohol dehydrogenase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,565,9660CT100.0% 211.6 / NA 65*427* (TAG→TAA) yjiNzinc‑type alcohol dehydrogenase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/31);  total (34/31)

ACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATT  >  NC_000913/4565840‑4566085
                                                                                                                              |                                                                                                                       
aCAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                             >  2:729142/1‑139 (MQ=255)
   aGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                          >  2:1142864/1‑139 (MQ=255)
        cATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGaa                                                                                                     <  2:478524/139‑1 (MQ=255)
          tGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACa                                                                                                   <  1:1142864/139‑1 (MQ=255)
          tGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACa                                                                                                   <  1:884735/139‑1 (MQ=255)
                           aCCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGAc                                                                                  >  1:514526/1‑139 (MQ=255)
                              aCGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                               <  1:196250/139‑1 (MQ=255)
                                              agagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGcc                                                               >  2:227377/1‑139 (MQ=255)
                                                          attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                             <  2:639686/81‑1 (MQ=255)
                                                          attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                             >  1:639686/1‑81 (MQ=255)
                                                            taCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCa                                                 >  1:1207640/1‑139 (MQ=255)
                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACaa                                                       >  2:522259/1‑128 (MQ=255)
                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACaa                                                       <  1:522259/128‑1 (MQ=255)
                                                                   cTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                          <  1:309204/139‑1 (MQ=255)
                                                                   cTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                          <  2:514526/139‑1 (MQ=255)
                                                                   cTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                          >  2:309204/1‑139 (MQ=255)
                                                                         gTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAGTTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGt                                    >  1:820514/1‑139 (MQ=255)
                                                                             ggTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                         >  2:568689/1‑114 (MQ=255)
                                                                             ggTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                         <  1:568689/114‑1 (MQ=255)
                                                                              gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                                <  2:1173106/122‑1 (MQ=255)
                                                                              gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                                >  1:1173106/1‑122 (MQ=255)
                                                                              gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGaca                               >  1:1190333/1‑139 (MQ=255)
                                                                              gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGaca                               >  1:1021968/1‑139 (MQ=255)
                                                                                    ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         <  1:278863/139‑1 (MQ=255)
                                                                                    ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         <  2:1207640/139‑1 (MQ=255)
                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                               >  1:113382/1‑81 (MQ=255)
                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                               <  2:113382/81‑1 (MQ=255)
                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                    >  1:351616/1‑92 (MQ=255)
                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                    <  2:351616/92‑1 (MQ=255)
                                                                                         ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                 >  1:1020638/1‑94 (MQ=255)
                                                                                         ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                 <  2:1020638/94‑1 (MQ=255)
                                                                                           ctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCa                  >  2:467467/1‑139 (MQ=255)
                                                                                            tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGAc                                                                                  >  2:981266/1‑74 (MQ=255)
                                                                                            tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGAc                                                                                  <  1:981266/74‑1 (MQ=255)
                                                                                            tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              >  2:1027932/1‑78 (MQ=255)
                                                                                            tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              <  1:1027932/78‑1 (MQ=255)
                                                                                            tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAata                                                                          >  1:151689/1‑82 (MQ=255)
                                                                                            tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAata                                                                          <  2:151689/82‑1 (MQ=255)
                                                                                              tcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                                  <  1:335909/104‑1 (MQ=255)
                                                                                              tcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                                  >  2:335909/1‑104 (MQ=255)
                                                                                                 cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                    <  1:268003/83‑1 (MQ=255)
                                                                                                 cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                    >  2:268003/1‑83 (MQ=255)
                                                                                                 cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCg                                                    >  2:723855/1‑99 (MQ=255)
                                                                                                 cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCg                                                    <  1:723855/99‑1 (MQ=255)
                                                                                                    ttAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGc         >  1:957139/1‑139 (MQ=255)
                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                         <  2:374521/89‑1 (MQ=255)
                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                         >  1:374521/1‑89 (MQ=255)
                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                               >  1:1188724/1‑99 (MQ=255)
                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                               <  2:1188724/99‑1 (MQ=255)
                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         <  1:713191/121‑1 (MQ=255)
                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         >  2:713191/1‑121 (MQ=255)
                                                                                                        ccACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGTCACGAATAAACTGCAGATCTTTGCCGat     >  2:730478/1‑139 (MQ=255)
                                                                                                         cACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         >  2:131810/1‑118 (MQ=255)
                                                                                                         cACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         <  1:131810/118‑1 (MQ=255)
                                                                                                           cTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATAtt  <  2:957139/139‑1 (MQ=255)
                                                                                                            ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAGAATTAg                                                                 >  2:757327/1‑75 (MQ=255)
                                                                                                            ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                 <  1:757327/75‑1 (MQ=255)
                                                                                                                  tttCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              >  2:713528/1‑56 (MQ=255)
                                                                                                                  tttCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              <  1:713528/56‑1 (MQ=255)
                                                                                                                     cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                 <  1:35701/66‑1 (MQ=255)
                                                                                                                     cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                 >  2:35701/1‑66 (MQ=255)
                                                                                                                     cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCa                  <  2:754987/113‑1 (MQ=255)
                                                                                                                     cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCa                  >  1:754987/1‑113 (MQ=255)
                                                                                                                      aTGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                          >  2:757812/1‑88 (MQ=255)
                                                                                                                      aTGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                          <  1:757812/88‑1 (MQ=255)
                                                                                                                              |                                                                                                                       
ACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATT  >  NC_000913/4565840‑4566085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A