Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
382,579 |
G→A |
*302* (TAG→TAA) |
insD1 → |
IS2 transposase TnpB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 382,579 | 0 | G | A | 100.0%
| 184.3
/ NA
| 58 | *302* (TAG→TAA) | insD1 | IS2 transposase TnpB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/29); total (29/29) |
CAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATT > NC_000913/382455‑382702
|
cAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAAtt < 1:139223/139‑1 (MQ=11)
cGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGATTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTAt > 1:1022900/1‑139 (MQ=25)
ttCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCa > 2:1376520/1‑139 (MQ=37)
tataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAGTCCAATTACCTATCAGGCAGTTTg < 1:1130114/139‑1 (MQ=255)
taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg > 1:923202/1‑139 (MQ=255)
aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt > 1:1268939/1‑139 (MQ=255)
ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg > 2:658803/1‑131 (MQ=255)
ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg < 1:658803/131‑1 (MQ=255)
gTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAgg > 2:287535/1‑110 (MQ=255)
gTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAgg < 1:287535/110‑1 (MQ=255)
gcgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGaa > 1:1161770/1‑139 (MQ=255)
ggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACgg < 2:923202/139‑1 (MQ=255)
gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGTTAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATg < 2:1111882/139‑1 (MQ=255)
gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATg < 2:1268939/139‑1 (MQ=255)
ctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCa < 1:1453957/133‑1 (MQ=255)
ctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCa > 2:1453957/1‑133 (MQ=255)
cgcCTCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGAcc < 2:1078897/118‑1 (MQ=255)
cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGATATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGAcc > 1:1078897/1‑118 (MQ=255)
gCGGCAGTGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCTATTACCTATCAGGCAGt > 1:1095037/1‑80 (MQ=17)
gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGt < 2:1095037/80‑1 (MQ=255)
ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa < 2:741156/86‑1 (MQ=255)
ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa > 1:741156/1‑86 (MQ=255)
aGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGAt < 1:177391/139‑1 (MQ=255)
cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATCTAAGGGCAAGTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaa > 1:1027947/1‑139 (MQ=255)
cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCgcgc > 1:1314882/1‑133 (MQ=255)
cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCgcgc < 2:1314882/133‑1 (MQ=255)
tGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCt < 1:1376520/139‑1 (MQ=255)
ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcgc > 2:152562/1‑66 (MQ=255)
ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcgc < 1:152562/66‑1 (MQ=255)
ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt < 1:1155256/70‑1 (MQ=255)
ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt > 2:1155256/1‑70 (MQ=255)
ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGc > 2:909577/1‑139 (MQ=255)
ttAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa < 2:422376/78‑1 (MQ=255)
ttAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa > 1:422376/1‑78 (MQ=255)
aaGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa > 2:1402579/1‑76 (MQ=255)
aaGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa < 1:1402579/76‑1 (MQ=255)
aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGttt > 2:659599/1‑139 (MQ=255)
aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGt < 2:1180500/137‑1 (MQ=255)
aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCCATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGt > 1:1180500/1‑137 (MQ=255)
gATAACAGATGTCTTGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATGACTACCGGACCCTGAATAACGGCAACATGACAAATACGGGTATTCGCGCCGCTAACAACTTCACCTGCCGTTtcat < 2:1022900/139‑1 (MQ=255)
gATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcat < 1:659599/139‑1 (MQ=255)
aTAACAGATGTCTGGAAGTATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcatc < 2:1027947/139‑1 (MQ=255)
tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCt > 1:1378874/1‑127 (MQ=255)
tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCt < 2:1378874/127‑1 (MQ=255)
tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcatca > 2:642854/1‑139 (MQ=255)
aTGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAAtt > 1:1499653/1‑139 (MQ=255)
tGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa > 1:1112697/1‑55 (MQ=255)
tGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa < 2:1112697/55‑1 (MQ=255)
tGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcatc > 2:500926/1‑131 (MQ=255)
tGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcatc < 1:500926/131‑1 (MQ=255)
gTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAcc > 2:1280827/1‑79 (MQ=255)
gTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAcc < 1:1280827/79‑1 (MQ=255)
tGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt > 2:1522780/1‑49 (MQ=255)
tGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt < 1:1522780/49‑1 (MQ=255)
tGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaac > 2:454991/1‑109 (MQ=255)
tGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaac < 1:454991/109‑1 (MQ=255)
gAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACtt < 2:1320783/109‑1 (MQ=255)
gAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACtt > 1:1320783/1‑109 (MQ=255)
|
CAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATT > NC_000913/382455‑382702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
