Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	382,579	G→A	302 (TAG→TAA)	insD1 →	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	382,579	0	G	A	100.0%	89.0 / NA	29	302 (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/13); total (16/13)

ATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTC  >  NC_000913/382480‑382710

aTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                                    <  1:587600/133‑1 (MQ=255)

aTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                                    >  2:587600/1‑133 (MQ=255)

  ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCAc                                                                                            >  2:731296/1‑139 (MQ=255)

  ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCAc                                                                                            >  1:592278/1‑139 (MQ=255)

      tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                                        <  2:592278/139‑1 (MQ=255)

        cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCgg                                                                                      <  1:731296/139‑1 (MQ=255)

                 cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAAt                                                                             >  2:777252/1‑139 (MQ=255)

                                                         ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGa                                                                >  1:242078/1‑112 (MQ=255)

                                                         ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGa                                                                <  2:242078/112‑1 (MQ=255)

                                                                    ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGc                          >  1:15177/1‑139 (MQ=255)

                                                                    ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGc                          >  2:147447/1‑139 (MQ=255)

                                                                         aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                               <  1:513750/65‑1 (MQ=255)

                                                                         aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                               >  2:513750/1‑65 (MQ=255)

                                                                         aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGttt                     >  1:437415/1‑139 (MQ=255)

                                                                            gATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcat                  <  2:437415/139‑1 (MQ=255)

                                                                              tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                                        >  1:220809/1‑67 (MQ=255)

                                                                              tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                                        <  2:220809/67‑1 (MQ=255)

                                                                              tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACa                                                              >  2:296870/1‑93 (MQ=255)

                                                                              tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACa                                                              <  1:296870/93‑1 (MQ=255)

                                                                                   gATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAgg                                                                                                             >  1:681043/1‑41 (MQ=255)

                                                                                   gATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAgg                                                                                                             <  2:681043/41‑1 (MQ=255)

                                                                                    aTGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                             <  1:717033/56‑1 (MQ=255)

                                                                                    aTGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                             >  2:717033/1‑56 (MQ=255)

                                                                                      gTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                                        >  1:784578/1‑59 (MQ=255)

                                                                                      gTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                                        <  2:784578/59‑1 (MQ=255)

                                                                                       tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt                                                                  >  2:229327/1‑80 (MQ=255)

                                                                                       tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt                                                                  <  1:229327/80‑1 (MQ=255)

                                                                                       tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTAcc       >  1:786412/1‑139 (MQ=255)

                                                                                            aaaTATAAGGGCCAATCCAATTACCTATCCGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACTGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCGGCAGTTTCATCAATAATTACCTGGTc  <  2:719318/139‑1 (MQ=255)

ATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTC  >  NC_000913/382480‑382710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A