Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 662,153 C→A E42D (GAG→GAT)  lipB ← octanoyltransferase, octanoyl‑[ACP]:protein N‑octanoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913662,1530CA100.0% 209.3 / NA 65E42D (GAG→GAT) lipBoctanoyltransferase, octanoyl‑[ACP]:protein N‑octanoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/32);  total (33/32)

CATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCAT  >  NC_000913/662024‑662286
                                                                                                                                 |                                                                                                                                     
catCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAccagc                                                                                                                              <  1:943153/139‑1 (MQ=255)
 atCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGAccagcc                                                                                                                             <  1:1393117/139‑1 (MQ=255)
         ttGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtca                                                                                                                     >  1:200688/1‑139 (MQ=255)
           gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                   <  2:999754/139‑1 (MQ=255)
             cccGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCaa                                                                                                                 >  2:526910/1‑139 (MQ=255)
                 gCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGt                                                                                                             >  1:774428/1‑139 (MQ=255)
                      tGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcat                                                                                                                    >  1:92018/1‑127 (MQ=255)
                      tGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcat                                                                                                                    <  2:92018/127‑1 (MQ=255)
                            gTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATcgc                                                                                                  <  2:200688/139‑1 (MQ=255)
                            gTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATcgc                                                                                                  >  1:283346/1‑139 (MQ=255)
                                ccTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGt                                                                                              <  2:774428/139‑1 (MQ=255)
                                 cTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTa                                                                                             >  2:30845/1‑139 (MQ=255)
                                  tGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTAt                                                                                            >  1:18298/1‑139 (MQ=255)
                                    cccACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcat                                                                                                                    >  2:23475/1‑113 (MQ=255)
                                    cccACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcat                                                                                                                    <  1:23475/113‑1 (MQ=255)
                                       aCCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTg                                                                                       <  1:1301220/139‑1 (MQ=255)
                                                 ctctGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             <  2:1151349/139‑1 (MQ=255)
                                                         cACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatca                                                                                                                  <  1:288764/94‑1 (MQ=255)
                                                         cACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatca                                                                                                                  >  2:288764/1‑94 (MQ=255)
                                                           ccGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGga                                                                   >  1:1479959/1‑139 (MQ=255)
                                                            cGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             <  1:1402991/128‑1 (MQ=255)
                                                            cGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             >  2:1402991/1‑128 (MQ=255)
                                                            cGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGgag                                                                  <  2:18298/139‑1 (MQ=255)
                                                                             ttAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTg                                                 <  1:526910/139‑1 (MQ=255)
                                                                                 aaTGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGa                                             <  2:283346/139‑1 (MQ=255)
                                                                                     tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTg                                                                                       <  1:343895/93‑1 (MQ=255)
                                                                                     tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTg                                                                                       >  2:343895/1‑93 (MQ=255)
                                                                                     tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAt                                                                >  1:1109602/1‑116 (MQ=255)
                                                                                     tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAt                                                                <  2:1109602/116‑1 (MQ=255)
                                                                                     tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGa                                         >  2:1325440/1‑139 (MQ=255)
                                                                                     tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGa                                         >  1:393973/1‑139 (MQ=255)
                                                                                       cTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatca                                                                                                                  <  1:1512462/64‑1 (MQ=255)
                                                                                       cTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatca                                                                                                                  >  2:1512462/1‑64 (MQ=255)
                                                                                                    gcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAAtt                                                                                   >  1:189455/1‑82 (MQ=255)
                                                                                                    gcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAAtt                                                                                   <  2:189455/82‑1 (MQ=255)
                                                                                                    gcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAAt                                                                                    >  1:1618382/1‑81 (MQ=255)
                                                                                                    gcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAAt                                                                                    <  2:1618382/81‑1 (MQ=255)
                                                                                                    gcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTg                                                                      >  1:1147336/1‑95 (MQ=255)
                                                                                                    gcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTg                                                                      <  2:1147336/95‑1 (MQ=255)
                                                                                                      ctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAAtt                        >  2:232335/1‑139 (MQ=255)
                                                                                                         aCCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCaa                                                                                                                 >  1:1384040/1‑47 (MQ=255)
                                                                                                         aCCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCaa                                                                                                                 <  2:1384040/47‑1 (MQ=255)
                                                                                                            ttGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATcc                  <  2:393973/139‑1 (MQ=255)
                                                                                                               ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCTAGAGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGa               >  1:750836/1‑139 (MQ=255)
                                                                                                               ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATcc                  >  1:1287401/1‑136 (MQ=255)
                                                                                                               ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATcc                  <  2:1287401/136‑1 (MQ=255)
                                                                                                                   aaTACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACa           >  1:642368/1‑139 (MQ=255)
                                                                                                                    aTACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACaa          >  2:68269/1‑139 (MQ=255)
                                                                                                                     tACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTatcatc                                                                                                    <  1:559716/48‑1 (MQ=255)
                                                                                                                     tACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTatcatc                                                                                                    >  2:559716/1‑48 (MQ=255)
                                                                                                                        cGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             >  1:375547/1‑68 (MQ=255)
                                                                                                                        cGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             <  2:375547/68‑1 (MQ=255)
                                                                                                                        cGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAtt                                                               <  1:758771/82‑1 (MQ=255)
                                                                                                                        cGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAtt                                                               >  2:758771/1‑82 (MQ=255)
                                                                                                                        cGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGc                                       >  1:509267/1‑106 (MQ=255)
                                                                                                                        cGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGc                                       <  2:509267/106‑1 (MQ=255)
                                                                                                                          gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             <  2:1154954/66‑1 (MQ=255)
                                                                                                                          gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                             >  1:1154954/1‑66 (MQ=255)
                                                                                                                          gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGc    >  2:133093/1‑139 (MQ=255)
                                                                                                                          gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGc    >  1:449736/1‑139 (MQ=255)
                                                                                                                           aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa   >  2:459718/1‑139 (MQ=255)
                                                                                                                           aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa   <  2:449736/139‑1 (MQ=255)
                                                                                                                           aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa   <  2:750836/139‑1 (MQ=255)
                                                                                                                           aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa   <  1:30845/139‑1 (MQ=255)
                                                                                                                            tAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCAt  <  1:68269/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
CATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCAT  >  NC_000913/662024‑662286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A