Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,153	C→A	E42D (GAG→GAT)	lipB ←	octanoyltransferase, octanoyl‑[ACP]:protein N‑octanoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,153	0	C	A	100.0%	163.8 / NA	51	E42D (GAG→GAT)	lipB	octanoyltransferase, octanoyl‑[ACP]:protein N‑octanoyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/24); total (27/24)

ACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCA  >  NC_000913/662023‑662285

acatCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGa                                                                                                                                  >  2:476112/1‑135 (MQ=255)

acatCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGa                                                                                                                                  <  1:476112/135‑1 (MQ=255)

      ccTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGAt                                                                                                                        >  2:662932/1‑139 (MQ=255)

      ccTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGAt                                                                                                                        <  1:687297/139‑1 (MQ=255)

          ttGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtca                                                                                                                    >  1:1119403/1‑139 (MQ=255)

            gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  1:155614/139‑1 (MQ=255)

            gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  2:581994/139‑1 (MQ=255)

            gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  2:1119403/139‑1 (MQ=255)

            gCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  1:864871/139‑1 (MQ=255)

             ccccGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatca                                                                                                                 <  1:662932/139‑1 (MQ=255)

                 ggCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcat                                                                                                                   >  2:902514/1‑133 (MQ=255)

                 ggCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcat                                                                                                                   <  1:902514/133‑1 (MQ=255)

                 ggCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAggg                                                                                                             >  1:96973/1‑139 (MQ=255)

                             gTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATcg                                                                                                  >  2:550214/1‑138 (MQ=255)

                             gTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATcg                                                                                                  <  1:550214/138‑1 (MQ=255)

                                 ccTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGt                                                                                             >  1:680637/1‑139 (MQ=255)

                                     cccACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  1:594944/114‑1 (MQ=255)

                                     cccACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  >  2:594944/1‑114 (MQ=255)

                                        aCCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTg                                                                                      <  1:32854/139‑1 (MQ=255)

                                                  ctctGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                            <  1:501232/139‑1 (MQ=255)

                                                   tctGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCAt                                                                           <  1:537613/139‑1 (MQ=255)

                                                         tCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTg                                                                     <  1:531902/139‑1 (MQ=255)

                                                            ccGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  >  1:622580/1‑91 (MQ=255)

                                                            ccGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTtcatc                                                                                                                  <  2:622580/91‑1 (MQ=255)

                                                            ccGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGga                                                                  >  2:783570/1‑139 (MQ=255)

                                                             cGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTatcatc                                                                                                   <  2:764267/105‑1 (MQ=255)

                                                             cGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTatcatc                                                                                                   >  1:764267/1‑105 (MQ=255)

                                                               gAAGATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGAt                                                               >  2:292484/1‑139 (MQ=255)

                                                                          ggCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTAt                                                                                           >  1:185806/1‑100 (MQ=255)

                                                                          ggCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTAt                                                                                           <  2:185806/100‑1 (MQ=255)

                                                                                      tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGAt                                                                                                                        >  1:914815/1‑59 (MQ=255)

                                                                                      tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGAt                                                                                                                        <  2:914815/59‑1 (MQ=255)

                                                                                           cGCTTTTCCTGCCTGACCTTCGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCgg                                                                                               <  2:315768/79‑1 (MQ=255)

                                                                                           cGCTTTTCCTGCCTGACCTTCGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCgg                                                                                               >  1:315768/1‑79 (MQ=255)

                                                                                                  cctgcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                            <  1:8343/91‑1 (MQ=255)

                                                                                                  cctgcctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCa                                                                            >  2:8343/1‑91 (MQ=255)

                                                                                                       ctgACCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAAtt                       >  1:201341/1‑139 (MQ=255)

                                                                                                          aCCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACaa                            >  2:947469/1‑131 (MQ=255)

                                                                                                          aCCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACaa                            <  1:947469/131‑1 (MQ=255)

                                                                                                          aCCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTa                    >  2:1221777/1‑139 (MQ=255)

                                                                                                          aCCTTGGGTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTa                    >  2:819070/1‑139 (MQ=255)

                                                                                                                ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGa              >  1:1037912/1‑139 (MQ=255)

                                                                                                                ggTGAATACCGGATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGa              >  2:47179/1‑139 (MQ=255)

                                                                                                                          ggATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAACCgg    >  2:852026/1‑139 (MQ=255)

                                                                                                                          ggATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACgg    >  1:990877/1‑139 (MQ=255)

                                                                                                                          ggATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACgg    <  2:1037912/139‑1 (MQ=255)

                                                                                                                          ggATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACgg    <  1:274652/139‑1 (MQ=255)

                                                                                                                           gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTa                                                                                                        >  2:871747/1‑38 (MQ=38)

                                                                                                                           gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTa                                                                                                        <  1:871747/38‑1 (MQ=38)

                                                                                                                           gATAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCGTGCATAGCCTGGGACATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGc   >  2:927350/1‑139 (MQ=255)

                                                                                                                            aTAGTGATCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCa  >  2:95272/1‑139 (MQ=255)

ACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCA  >  NC_000913/662023‑662285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A