Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,826,546 |
T→C |
E126E (GAA→GAG) |
astE ← |
succinylglutamate desuccinylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,826,546 | 0 | T | C | 98.6%
| 251.7
/ NA
| 71 | E126E (GAA→GAG) | astE | succinylglutamate desuccinylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (34/36); minor base G (1/0); total (35/36) |
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAC > NC_000913/1826422‑1826677
|
tgggAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCgcgcgc < 2:912945/138‑1 (MQ=255)
aGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCgcgcgc > 1:1570035/1‑139 (MQ=255)
aaTGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGa < 1:1223831/139‑1 (MQ=255)
gTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAg > 1:450984/1‑139 (MQ=255)
tGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTtctc > 2:1538393/1‑139 (MQ=255)
gTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCt < 2:797156/139‑1 (MQ=255)
ccGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaa > 2:117955/1‑139 (MQ=255)
ccGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaa > 2:713882/1‑139 (MQ=255)
aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACa < 2:334187/116‑1 (MQ=255)
aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACa > 1:334187/1‑116 (MQ=255)
aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATa > 1:1301713/1‑139 (MQ=255)
aCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg < 2:1570035/139‑1 (MQ=255)
cTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGc < 1:1538393/139‑1 (MQ=255)
tGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCt > 1:1450946/1‑139 (MQ=255)
ggATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg < 1:199222/101‑1 (MQ=255)
ggATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg > 2:199222/1‑101 (MQ=255)
tGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc < 2:637703/139‑1 (MQ=255)
tGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc < 2:450984/139‑1 (MQ=255)
gAGCCATGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGTTGCCAACg < 2:1084422/134‑1 (MQ=255)
gAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACg > 1:1084422/1‑134 (MQ=255)
gAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGc < 2:1301713/139‑1 (MQ=255)
gCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCa < 1:1658621/139‑1 (MQ=255)
ccACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCaa > 1:830114/1‑139 (MQ=255)
aCGAATTGCGGTATGTAGATCATGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAAc > 1:1063397/1‑139 (MQ=255)
ttGCGGTATGTAGATCATGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCg < 2:1063397/139‑1 (MQ=255)
tGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa < 1:117955/139‑1 (MQ=255)
aGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGa > 1:1066954/1‑78 (MQ=255)
aGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGa < 2:1066954/78‑1 (MQ=255)
ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg > 2:1304789/1‑66 (MQ=255)
ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg < 1:1304789/66‑1 (MQ=255)
ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGcc < 1:1048072/112‑1 (MQ=255)
ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGcc > 2:1048072/1‑112 (MQ=255)
ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa > 2:403106/1‑139 (MQ=255)
ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa < 2:1151278/139‑1 (MQ=255)
ggTGACAGCGCACAGATTCTTTGCCCTGGTCATCAAAATCTTCCAGGCACTGGTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCTAACGACCGCCACACATTCGATTCATATAGCAATGGCa > 1:1151278/1‑139 (MQ=255)
tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAAt > 1:876590/1‑139 (MQ=255)
tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAAt < 2:830114/139‑1 (MQ=255)
tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAAt > 2:1356361/1‑139 (MQ=255)
cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAgg < 1:1126332/70‑1 (MQ=255)
cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAgg > 2:1126332/1‑70 (MQ=255)
cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCat < 1:1305738/122‑1 (MQ=255)
cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCat > 2:1305738/1‑122 (MQ=255)
caGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTccc > 2:1306972/1‑139 (MQ=255)
gATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGcc < 1:1447969/54‑1 (MQ=255)
gATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGcc > 2:1447969/1‑54 (MQ=255)
cTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCtt < 2:1450946/139‑1 (MQ=255)
cTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCtt < 1:1589754/139‑1 (MQ=255)
cTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCtt > 1:952819/1‑139 (MQ=255)
tttGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTc > 2:947645/1‑139 (MQ=255)
cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTTGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCa > 1:1488575/1‑139 (MQ=255)
cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa < 2:1256135/109‑1 (MQ=255)
cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTa > 1:1256135/1‑109 (MQ=255)
cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCa > 1:1068054/1‑139 (MQ=255)
tgttCATAAAAATCTTCCAGCCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATACGa < 1:1075019/93‑1 (MQ=255)
tGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa > 2:1075019/1‑96 (MQ=255)
ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGcc < 2:284185/85‑1 (MQ=255)
ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGcc > 1:284185/1‑85 (MQ=255)
ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCa > 2:535470/1‑86 (MQ=255)
ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCa < 1:535470/86‑1 (MQ=255)
ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCat > 2:155396/1‑100 (MQ=255)
ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCat < 1:155396/100‑1 (MQ=255)
gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGc < 1:522635/56‑1 (MQ=255)
gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGc > 2:522635/1‑56 (MQ=255)
tCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCaggag < 1:1356361/139‑1 (MQ=255)
aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAggagga < 2:1615699/139‑1 (MQ=255)
aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAggagga < 1:713882/139‑1 (MQ=255)
aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAggagga < 2:876590/139‑1 (MQ=255)
tCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa > 1:1447176/1‑101 (MQ=255)
tCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa < 2:1447176/101‑1 (MQ=255)
ggCACTGCTCCAGTTTGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAc < 2:1488575/139‑1 (MQ=255)
ggCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAc > 1:578234/1‑139 (MQ=255)
|
AGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAC > NC_000913/1826422‑1826677
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
