Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,826,546	T→C	E126E (GAA→GAG)	astE ←	succinylglutamate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,826,546	0	T	C	100.0%	226.2 / NA	63	E126E (GAA→GAG)	astE	succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (35/28); total (35/28)

TCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAT  >  NC_000913/1826430‑1826674

tCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAgg                                                                                                            >  1:1000939/1‑139 (MQ=255)

    gTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTtc                                                                                                        >  2:1827240/1‑139 (MQ=255)

        ccgTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGAGCGCCCGACAGGTTTCTCCg                                                                                                    <  1:1105876/137‑1 (MQ=255)

           tAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCtt                                                                                                 <  1:977435/139‑1 (MQ=255)

                cacCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGc                                                                                            >  2:478372/1‑139 (MQ=255)

                  ccGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaa                                                                                          >  2:1028712/1‑139 (MQ=255)

                  ccGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaa                                                                                          >  1:1740835/1‑139 (MQ=255)

                     aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATa                                                                                       >  1:1765648/1‑139 (MQ=255)

                      aCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                      <  1:478372/139‑1 (MQ=255)

                               gCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACg                                                                             <  2:166314/139‑1 (MQ=255)

                               gCAAGGAACCACGCATTGCGGTATGTAGATCACGGTGCCAGCGCACAGATTCTTTGCCCTGGGCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTACAGCAAATAGCTGCCAACg                                                                             >  1:1231008/1‑139 (MQ=255)

                                       ccACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg                                                                                                                       <  2:309675/89‑1 (MQ=255)

                                       ccACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg                                                                                                                       >  1:309675/1‑89 (MQ=255)

                                          cGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                      <  2:1756442/119‑1 (MQ=255)

                                          cGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                      >  1:1756442/1‑119 (MQ=255)

                                               tGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa                                                             <  2:1740835/139‑1 (MQ=255)

                                                gCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGa                                                                                                                >  1:549265/1‑87 (MQ=255)

                                                gCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGa                                                                                                                <  2:549265/87‑1 (MQ=255)

                                                    tATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCat                                                        <  1:1028712/139‑1 (MQ=255)

                                                            tCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTtc                                                                                                        >  1:1783943/1‑83 (MQ=255)

                                                            tCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTtc                                                                                                        <  2:1783943/83‑1 (MQ=255)

                                                            tCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTAt                                                <  2:1000939/139‑1 (MQ=255)

                                                              aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATgg                                              >  2:1072636/1‑139 (MQ=255)

                                                              aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATgg                                              >  1:826284/1‑139 (MQ=255)

                                                              aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATgg                                              >  2:944888/1‑139 (MQ=255)

                                                                ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCa                                                                                              >  2:1096082/1‑89 (MQ=255)

                                                                ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCa                                                                                              <  1:1096082/89‑1 (MQ=255)

                                                                ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                            >  2:316411/1‑139 (MQ=255)

                                                                ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                            >  2:362436/1‑139 (MQ=255)

                                                                ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                            >  2:494614/1‑139 (MQ=255)

                                                                  tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCt                                                  <  1:582245/131‑1 (MQ=255)

                                                                  tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCt                                                  >  2:582245/1‑131 (MQ=255)

                                                                     cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAt                                                                 <  1:1289330/113‑1 (MQ=255)

                                                                     cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACAt                                                                 >  2:1289330/1‑113 (MQ=255)

                                                                     cAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCACTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAAc                                       >  2:963480/1‑139 (MQ=255)

                                                                        cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCa                                                                                              >  2:38035/1‑81 (MQ=255)

                                                                        cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCa                                                                                              <  1:38035/81‑1 (MQ=255)

                                                                        cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGtt                                    <  2:826284/139‑1 (MQ=255)

                                                                        cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGtt                                    >  2:1871891/1‑139 (MQ=255)

                                                                           acaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTcc                                 <  1:316411/139‑1 (MQ=255)

                                                                                     tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGtt                                    <  2:706628/126‑1 (MQ=255)

                                                                                     tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGtt                                    >  1:706628/1‑126 (MQ=255)

                                                                                         cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg                                                                                                                       <  1:135525/39‑1 (MQ=38)

                                                                                         cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcg                                                                                                                       >  2:135525/1‑39 (MQ=38)

                                                                                           ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa                                                             >  2:392895/1‑95 (MQ=255)

                                                                                           ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGa                                                             <  1:392895/95‑1 (MQ=255)

                                                                                           ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagg                 >  2:901580/1‑139 (MQ=255)

                                                                                           ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagg                 >  1:502476/1‑139 (MQ=255)

                                                                                           ggTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagg                 >  1:581590/1‑139 (MQ=255)

                                                                                            gTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg                                                                                                                     >  1:1679651/1‑38 (MQ=255)

                                                                                            gTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg                                                                                                                     <  2:1679651/38‑1 (MQ=255)

                                                                                            gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGAt                                                            >  1:113535/1‑95 (MQ=255)

                                                                                            gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGAt                                                            <  2:113535/95‑1 (MQ=255)

                                                                                            gTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCagga                >  1:1013495/1‑139 (MQ=255)

                                                                                             tCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCaggag               <  1:944888/139‑1 (MQ=255)

                                                                                             tCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCaggag               <  1:1072636/139‑1 (MQ=255)

                                                                                               aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                      <  1:1035381/66‑1 (MQ=255)

                                                                                               aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAg                                                                                      >  2:1035381/1‑66 (MQ=255)

                                                                                                 aaaaaTCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAtt           >  2:1394075/1‑139 (MQ=255)

                                                                                                      tCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCACTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa      <  1:963480/139‑1 (MQ=255)

                                                                                                          ccAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt  <  1:1827240/139‑1 (MQ=255)

                                                                                                           cAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCtt                                                                                                 <  2:998193/43‑1 (MQ=255)

                                                                                                           cAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCtt                                                                                                 >  1:998193/1‑43 (MQ=255)

TCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAT  >  NC_000913/1826430‑1826674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A