Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 524,158 C→T L300L (CTG→TTG)  rhsD → Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913524,1580CT100.0% 187.3 / NA 59L300L (CTG→TTG) rhsDRhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

CCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCT  >  NC_000913/524037‑524277
                                                                                                                         |                                                                                                                       
ccGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCa                                                                                                        <  2:321315/139‑1 (MQ=255)
   cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATAc                                                                                                     >  1:74819/1‑139 (MQ=255)
      ctctTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCg                                                                                                  <  1:933344/139‑1 (MQ=255)
                 cttcttctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                  <  1:890700/112‑1 (MQ=255)
                 cttcttctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                  >  2:890700/1‑112 (MQ=255)
                          aCAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGcc                                                                              >  2:779035/1‑139 (MQ=255)
                              ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                          >  1:424149/1‑139 (MQ=255)
                              ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                          <  2:23419/139‑1 (MQ=255)
                              ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                          >  2:74316/1‑139 (MQ=255)
                              ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                          >  1:1063857/1‑139 (MQ=255)
                               tCCCGCCCGCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTgg                                                                         <  2:1063857/139‑1 (MQ=255)
                                 ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  1:74316/139‑1 (MQ=255)
                                      ctctctCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                  <  2:471599/139‑1 (MQ=255)
                                        ctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTaca                                                                >  2:751423/1‑139 (MQ=255)
                                         tctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATg                                                                                                                    <  1:36314/86‑1 (MQ=255)
                                         tctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATg                                                                                                                    >  2:36314/1‑86 (MQ=255)
                                               cctcagcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                  <  2:111980/82‑1 (MQ=255)
                                               cctcagcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                  >  1:111980/1‑82 (MQ=255)
                                                   agcGTTGCCCGACACAATGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCTCCTTTCGGCGGTTTTGTTGATGCACGACCCGGCATACCCGGAGAGTCTGCCCGCTGCGCCACTGGTTCGGTACACGTAAACGGaa                                                     >  2:435435/1‑139 (MQ=255)
                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  2:398470/1‑121 (MQ=255)
                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  1:1067811/1‑121 (MQ=255)
                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  2:1067811/121‑1 (MQ=255)
                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  1:398470/121‑1 (MQ=255)
                                                   agcGTTCCCCGACACACTGCCCGGTACCAAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                    <  2:56632/124‑1 (MQ=255)
                                                   agcGTTCCCCGACACACTGCCCGGTACCAAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                    >  1:56632/1‑124 (MQ=255)
                                                                   cTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     <  1:779035/139‑1 (MQ=255)
                                                                   cTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     >  2:867552/1‑139 (MQ=255)
                                                                    tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATGCGGAAGCCGGTGAACTGATGGc                                    >  2:976295/1‑139 (MQ=255)
                                                                    tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     <  1:696372/138‑1 (MQ=255)
                                                                    tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     >  2:696372/1‑138 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGAc                                                                                                              <  2:197132/63‑1 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGAc                                                                                                              >  1:197132/1‑63 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  <  2:527033/91‑1 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  >  1:527033/1‑91 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  1:762069/1‑102 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  2:762069/102‑1 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     >  2:244321/1‑136 (MQ=255)
                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     <  1:244321/136‑1 (MQ=255)
                                                                           tACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGa                                                                                                               <  1:586500/57‑1 (MQ=255)
                                                                           tACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGa                                                                                                               >  2:586500/1‑57 (MQ=255)
                                                                           tACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGc                                    <  2:354993/132‑1 (MQ=255)
                                                                           tACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGc                                    >  1:354993/1‑132 (MQ=255)
                                                                              cGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  <  2:272350/83‑1 (MQ=255)
                                                                              cGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                  >  1:272350/1‑83 (MQ=255)
                                                                              cGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGAc                          <  2:424149/139‑1 (MQ=255)
                                                                                  tACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     >  2:403942/1‑124 (MQ=255)
                                                                                  tACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     <  1:403942/124‑1 (MQ=255)
                                                                                     ggCCCCGACAGGGGTATCCGCCTTTCGGCGGAGTGGTTGATGCa                                                                                                                  >  2:116031/1‑44 (MQ=255)
                                                                                     ggCCCCGACAGGGGTATCCGCCTTTCGGCGGAGTGGTTGATGCa                                                                                                                  <  1:116031/44‑1 (MQ=255)
                                                                                       ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAAt                 >  2:438138/1‑139 (MQ=255)
                                                                                            aCAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTac                                                                 >  1:679964/1‑86 (MQ=255)
                                                                                            aCAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTac                                                                 <  2:679964/86‑1 (MQ=255)
                                                                                                 ggTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     >  2:748107/1‑109 (MQ=255)
                                                                                                 ggTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                     <  1:748107/109‑1 (MQ=255)
                                                                                                      ccGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCt  <  1:867552/139‑1 (MQ=255)
                                                                                                      ccGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCt  >  1:768851/1‑139 (MQ=255)
                                                                                                          cTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  2:496564/1‑66 (MQ=255)
                                                                                                          cTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       <  1:496564/66‑1 (MQ=255)
                                                                                                                      tgGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                       >  1:978178/1‑54 (MQ=255)
                                                                                                                         |                                                                                                                       
CCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCT  >  NC_000913/524037‑524277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A