Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,089,570	T→C	E96E (GAA→GAG)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,089,570	0	T	C	96.9%	101.6 / ‑6.4	32	E96E (GAA→GAG)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (13/18); total (14/18)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTT  >  NC_000913/1089455‑1089679

cagGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGAc                                                                                        <  1:148890/139‑1 (MQ=255)

cagGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGAc                                                                                        >  2:549582/1‑139 (MQ=255)

   gCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCgg                                                                                     >  1:741669/1‑139 (MQ=255)

     cACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGtt                                                                                   <  1:957116/139‑1 (MQ=255)

     cACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGtt                                                                                   >  2:649810/1‑139 (MQ=255)

           aaGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAAc                                                                             <  2:840931/139‑1 (MQ=255)

                  gaagaaTTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTtctc                                                                      >  2:592953/1‑139 (MQ=255)

                     gaaTTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTcgcg                                                                   <  1:592953/139‑1 (MQ=255)

                             aaGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGTCAgg                                                           >  1:306815/1‑139 (MQ=255)

                                  gcgGGTATAAAAACTCTGGTAGACGTCATCAAAAGTCAGCACTACAGCTTCTTACGATAGCGATGTTTATCCTCGATGTGCTTCTCGAATCTGAGAAATACTGACCGGTTGATTACAGTTCTCGCGCAGCCAGCGAAAt                                                      >  2:282006/1‑139 (MQ=255)

                                        ataAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTTCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCa                                                <  1:282006/139‑1 (MQ=255)

                                          aaaaaCTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGTCAGGCAAATTGTTCACg                                              <  2:306815/139‑1 (MQ=255)

                                          aaaaaCTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                              <  1:649810/139‑1 (MQ=255)

                                          aaaaaCTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACg                                              <  1:549582/139‑1 (MQ=255)

                                                tctGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGc                                        >  1:45001/1‑139 (MQ=255)

                                                   ggTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGc                                                                                                 <  1:510487/79‑1 (MQ=255)

                                                   ggTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGc                                                                                                 >  2:510487/1‑79 (MQ=255)

                                                   ggTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGtt                                                                         >  1:731735/1‑103 (MQ=255)

                                                   ggTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGtt                                                                         <  2:731735/103‑1 (MQ=255)

                                                   ggTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGa                                     >  2:1021594/1‑139 (MQ=255)

                                                           tcatcaAAAGTCAGCTGTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCGCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCAGGCAGTGCTGATGTCCGcg                             <  2:489024/139‑2 (MQ=255)

                                                           tcatcaAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCa                             <  2:2680/139‑1 (MQ=255)

                                                            catcaAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCa                                                                 <  1:1011475/102‑1 (MQ=255)

                                                            catcaAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCa                                                                 >  2:1011475/1‑102 (MQ=255)

                                                            catcaAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCAc                            <  2:680790/139‑1 (MQ=255)

                                                                           cTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTg             <  2:741669/139‑1 (MQ=255)

                                                                              cAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTc          >  1:519168/1‑139 (MQ=255)

                                                                                      tCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                  >  2:1044285/1‑43 (MQ=255)

                                                                                      tCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAg                                                                                                  <  1:1044285/43‑1 (MQ=255)

                                                                                      tCCGGTAGCGGTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCtt  <  2:519168/139‑1 (MQ=255)

                                                                                                gTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCg                                                                                      >  1:833795/1‑45 (MQ=255)

                                                                                                gTTTTCCTCCTCGATGTGCCTCACGAATTTGAGCAATACTGACCg                                                                                      <  2:833795/45‑1 (MQ=255)

CAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACGAATTTGAGCAATACTGACCGGTTGATAACCGTTCTCGCGCAGCCAGGCAAATTGTTCACGCAGTGCTGATGTCCGCACTGACATAAAACGCTGGTCGGCAGCTT  >  NC_000913/1089455‑1089679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A