Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,308	A→G	E151E (GAA→GAG)	opgD →	OPG biosynthetic periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,308	0	A	G	100.0%	166.9 / NA	47	E151E (GAA→GAG)	opgD	OPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/27); total (20/27)

GGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCA  >  NC_000913/1497186‑1497427

ggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCg                                                                                                         >  1:544377/1‑139 (MQ=255)

  aTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGc                                                                                                       >  2:13549/1‑139 (MQ=255)

    tCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCtt                                                                                                     >  2:44855/1‑139 (MQ=255)

     ccGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCttt                                                                                                    >  2:506968/1‑139 (MQ=255)

      cGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTg                                                                                                   >  2:805210/1‑139 (MQ=255)

          gccgcGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCgg                                                                                               >  2:13225/1‑139 (MQ=255)

           ccgcGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGt                                                                                              >  2:602145/1‑139 (MQ=255)

                 tCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc                                                                                        <  2:544377/139‑1 (MQ=255)

                                       gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGTTTTTCGCGTGTTTAAAGCCCCCGAACTgg                                                                  >  2:692594/1‑139 (MQ=255)

                                       gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTgg                                                                  >  1:126522/1‑139 (MQ=255)

                                       gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTgg                                                                  <  1:73151/139‑1 (MQ=255)

                                         aaCACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                <  2:812380/139‑1 (MQ=255)

                                         aaCACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                <  1:33923/139‑1 (MQ=255)

                                         aaCACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                <  2:506011/139‑1 (MQ=255)

                                         aaCACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                <  2:233242/139‑1 (MQ=255)

                                           cacaTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcgcg                                                              <  1:13225/139‑1 (MQ=255)

                                             caTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                            >  1:831059/1‑139 (MQ=255)

                                             caTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                            <  2:954155/139‑1 (MQ=255)

                                             caTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                            <  2:554703/139‑1 (MQ=255)

                                              aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                           <  2:126522/139‑1 (MQ=255)

                                                  ggCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTa                                                                                 <  2:984260/113‑1 (MQ=255)

                                                  ggCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTa                                                                                 >  1:984260/1‑113 (MQ=255)

                                                   gcgcGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATg                                                      <  1:44855/139‑1 (MQ=255)

                                                                     cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg                                                         <  1:454833/118‑1 (MQ=255)

                                                                     cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg                                                         >  2:454833/1‑118 (MQ=255)

                                                                     cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                                    <  1:805210/139‑1 (MQ=255)

                                                                                   cAACTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgc                      <  1:692594/139‑1 (MQ=255)

                                                                                      aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                   <  1:13549/139‑1 (MQ=255)

                                                                                           aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGa                                                        <  2:377630/97‑1 (MQ=255)

                                                                                           aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGa                                                        >  1:377630/1‑97 (MQ=255)

                                                                                           aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                   >  1:584198/1‑134 (MQ=255)

                                                                                           aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                   <  2:584198/134‑1 (MQ=255)

                                                                                           aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga              <  2:762218/139‑1 (MQ=255)

                                                                                               tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTgatgat          <  2:831059/139‑1 (MQ=255)

                                                                                                gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTg                                                                   >  2:429097/1‑81 (MQ=255)

                                                                                                gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTg                                                                   <  1:429097/81‑1 (MQ=255)

                                                                                                 cAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtgatga           <  1:70292/136‑1 (MQ=255)

                                                                                                 cAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtgatga           >  2:70292/1‑136 (MQ=255)

                                                                                                       ttGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCa  <  1:602145/139‑1 (MQ=255)

                                                                                                         gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                >  2:537419/1‑75 (MQ=255)

                                                                                                         gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                <  1:537419/75‑1 (MQ=255)

                                                                                                               aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                <  2:572929/69‑1 (MQ=255)

                                                                                                               aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                >  1:572929/1‑69 (MQ=255)

                                                                                                                aaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATg                                                      <  2:80084/78‑1 (MQ=255)

                                                                                                                aaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATg                                                      >  1:80084/1‑78 (MQ=255)

                                                                                                                     ttAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                   <  2:853377/108‑1 (MQ=255)

                                                                                                                     ttAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                   >  1:853377/1‑108 (MQ=255)

GGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCA  >  NC_000913/1497186‑1497427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A