Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,308	A→G	E151E (GAA→GAG)	opgD →	OPG biosynthetic periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,308	0	A	G	100.0%	183.5 / NA	51	E151E (GAA→GAG)	opgD	OPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/25); total (26/25)

TGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCC  >  NC_000913/1497178‑1497443

tgggaatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAg                                                                                                                                 >  1:470449/1‑139 (MQ=255)

    aatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGc                                                                                                                                     >  1:1185190/1‑131 (MQ=255)

    aatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGc                                                                                                                                     <  2:1185190/131‑1 (MQ=255)

           ttCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCt                                                                                                                      >  1:667410/1‑139 (MQ=255)

              cGTCGCCGCGTTCGTATGTTGTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCttgg                                                                                                                   >  2:771405/1‑137 (MQ=255)

                   ccgcGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGt                                                                                                              >  2:1089076/1‑139 (MQ=255)

                       gTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGACGCGTGAAATACACTTTCGCCCGGAGATGTTCAAATACAACGATGCAGGTATTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTc                                                                                                          >  1:836469/1‑139 (MQ=255)

                              tGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGtt                                                                                                   <  1:800053/139‑1 (MQ=255)

                                       tAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGcccc                                                                                          >  1:637123/1‑139 (MQ=255)

                                                    acaTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcgcgc                                                                             >  2:813736/1‑139 (MQ=255)

                                                      aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                           >  2:987460/1‑139 (MQ=255)

                                                      aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                           >  1:743694/1‑139 (MQ=255)

                                                      aTCTGGCGCGGGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                           <  1:1248886/139‑1 (MQ=255)

                                                       tCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGt                                                                          <  2:1126936/139‑1 (MQ=255)

                                                        cTGGCGCGTGAAATTCACTTTCGCCCTGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg                                                                         <  2:836469/139‑1 (MQ=255)

                                                             gcgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCGGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTATAGCCCCCGAACTGGCGCGCCGTGATgta                                                                    >  2:1073260/1‑139 (MQ=255)

                                                              cgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCgg                                                                                                                        >  1:736593/1‑86 (MQ=255)

                                                              cgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCgg                                                                                                                        <  2:736593/86‑1 (MQ=255)

                                                                tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAACACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTATCGCGTGTTTAAAGCCCTCGAACTGGCGCGCCGTGCTgtagta                                                                 >  2:1219944/1‑139 (MQ=255)

                                                                tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGccc                                                                                           <  1:211794/113‑1 (MQ=255)

                                                                tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGccc                                                                                           >  2:211794/1‑113 (MQ=255)

                                                                tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtagta                                                                 >  2:908876/1‑139 (MQ=255)

                                                                        aCTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTtct                                                         <  1:1089076/139‑1 (MQ=255)

                                                                            tCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgc                                                     >  2:1054930/1‑139 (MQ=255)

                                                                             cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACACAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                                                    <  1:1073260/139‑1 (MQ=255)

                                                                                ccGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGa                                                 <  2:1246655/139‑1 (MQ=255)

                                                                                            aaaTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg                                     <  1:1202850/139‑1 (MQ=255)

                                                                                                   aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga                              <  1:987460/139‑1 (MQ=255)

                                                                                                   aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga                              <  2:642364/139‑1 (MQ=255)

                                                                                                   aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga                              <  2:637123/139‑1 (MQ=255)

                                                                                                       tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                           >  2:119928/1‑90 (MQ=255)

                                                                                                       tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                           <  1:119928/90‑1 (MQ=255)

                                                                                                       tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg                                     >  2:861407/1‑128 (MQ=255)

                                                                                                       tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg                                     <  1:861407/128‑1 (MQ=255)

                                                                                                        gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                                            >  2:165241/1‑88 (MQ=255)

                                                                                                        gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                                            <  1:165241/88‑1 (MQ=255)

                                                                                                        gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa                         <  2:487474/139‑1 (MQ=255)

                                                                                                        gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa                         <  2:667410/139‑1 (MQ=255)

                                                                                                          aGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGGGAGTTATTTCCGCGCCGTTGATGATACa                       <  1:813736/139‑1 (MQ=255)

                                                                                                          aGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACa                       <  2:743694/139‑1 (MQ=255)

                                                                                                                 gATACAAAACAATTAGAGGGGCAAAGCTATCTCGGCTTTGCCGGTTTTCGCGTGTTTaaa                                                                                               <  2:781216/60‑1 (MQ=255)

                                                                                                                 gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTaaa                                                                                               >  1:781216/1‑60 (MQ=255)

                                                                                                                 gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt                                                                     <  1:79506/86‑1 (MQ=255)

                                                                                                                 gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt                                                                     >  2:79506/1‑86 (MQ=255)

                                                                                                                      aaaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                                                    >  1:1069529/1‑98 (MQ=255)

                                                                                                                      aaaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                                                    <  2:1069529/98‑1 (MQ=255)

                                                                                                                       aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGtt          >  1:701800/1‑139 (MQ=255)

                                                                                                                        aaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGttt         >  1:939437/1‑139 (MQ=255)

                                                                                                                        aaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGttt         >  2:894178/1‑139 (MQ=255)

                                                                                                                             ttAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg                                     >  2:994059/1‑106 (MQ=255)

                                                                                                                             ttAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg                                     <  1:994059/106‑1 (MQ=255)

                                                                                                                               agagGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc                                                                                                        >  1:737475/1‑37 (MQ=255)

                                                                                                                               agagGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATTCGGTTTGTCGGcc  <  1:771405/139‑1 (MQ=255)

TGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCC  >  NC_000913/1497178‑1497443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A