Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,391	G→A	G90S (GGC→AGC)	bdcR →	transcriptional repressor for divergent bdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,391	0	G	A	100.0%	239.3 / NA	75	G90S (GGC→AGC)	bdcR	transcriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (39/36); total (39/36)

ATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATC  >  NC_000913/4474257‑4474526

aTCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGa                                                                                                                                     >  1:669367/1‑139 (MQ=255)

    tGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                                 >  1:564700/1‑139 (MQ=255)

     ggTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGcc                                                                                                                                >  1:867367/1‑139 (MQ=255)

           aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                          >  1:386564/1‑139 (MQ=255)

           aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                          >  1:251563/1‑139 (MQ=255)

           aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                          >  2:31654/1‑139 (MQ=255)

           aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                          <  2:949896/139‑1 (MQ=255)

            aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                                 <  1:792672/131‑1 (MQ=255)

            aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                                 >  2:792672/1‑131 (MQ=255)

            aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                         <  1:552612/139‑1 (MQ=255)

            aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                         >  1:815413/1‑139 (MQ=255)

            aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                         <  1:775516/139‑1 (MQ=255)

            aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                         <  2:524947/139‑1 (MQ=255)

                cccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTa                                                                                                                     >  1:225583/1‑139 (MQ=255)

                      ccTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTaaaa                                                                                                               >  1:986472/1‑139 (MQ=255)

                            gcaggattttGGCAGTAAAGCTGGGTTATTTTGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcg                                                                                                         <  2:867367/133‑1 (MQ=255)

                               ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcggcg                                                                                                      <  2:188636/139‑1 (MQ=255)

                                gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAg                                                                                                                        <  2:785524/120‑1 (MQ=255)

                                gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAg                                                                                                                        >  1:785524/1‑120 (MQ=255)

                                gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgc                                                                                                     <  2:251563/139‑1 (MQ=255)

                                 cTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                    >  1:879172/1‑139 (MQ=255)

                                           tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGc                                                                                          >  1:596063/1‑139 (MQ=255)

                                                cTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCCTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCaaaa                                                                                     >  2:803813/1‑139 (MQ=255)

                                                           aGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcg                                                                          <  2:815413/139‑1 (MQ=255)

                                                            gCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCCTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                         <  1:803813/139‑1 (MQ=255)

                                                             ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgct                                                                        >  2:41551/1‑139 (MQ=255)

                                                                    cTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                 <  1:31654/139‑1 (MQ=255)

                                                                            aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                                  >  1:685348/1‑66 (MQ=255)

                                                                            aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                                  <  2:685348/66‑1 (MQ=255)

                                                                            aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGTTGCgct                                                                        <  2:804688/124‑1 (MQ=255)

                                                                            aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGTTGCgct                                                                        >  1:804688/1‑124 (MQ=255)

                                                                             tACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                        <  2:527201/139‑1 (MQ=255)

                                                                             tACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                        >  1:527201/1‑139 (MQ=255)

                                                                                gTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAg                                                     >  2:939903/1‑139 (MQ=255)

                                                                                 tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                                 <  2:94128/62‑1 (MQ=255)

                                                                                 tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                                 >  1:94128/1‑62 (MQ=255)

                                                                                 tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAgg                                                    >  2:303508/1‑139 (MQ=255)

                                                                                  cGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGt                                                   >  1:80613/1‑139 (MQ=255)

                                                                                   ggTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTa                                                  >  2:666449/1‑139 (MQ=255)

                                                                                     tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGtt                                                                                                                           >  2:243897/1‑64 (MQ=255)

                                                                                     tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGtt                                                                                                                           <  1:243897/64‑1 (MQ=255)

                                                                                     tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgct                                                                        <  2:1012055/115‑1 (MQ=255)

                                                                                     tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgct                                                                        >  1:1012055/1‑115 (MQ=255)

                                                                                             cTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        <  2:669367/139‑1 (MQ=255)

                                                                                             cTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        <  2:80613/139‑1 (MQ=255)

                                                                                             cTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        <  1:666449/139‑1 (MQ=255)

                                                                                             cTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        <  2:386564/139‑1 (MQ=255)

                                                                                             cTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        <  1:553659/139‑1 (MQ=255)

                                                                                              tATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        >  1:112128/1‑138 (MQ=255)

                                                                                              tATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                        <  2:112128/138‑1 (MQ=255)

                                                                                               aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                          >  2:984424/1‑55 (MQ=255)

                                                                                               aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                          <  1:984424/55‑1 (MQ=255)

                                                                                               aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAg                                                                                                                        >  2:795202/1‑57 (MQ=255)

                                                                                               aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAg                                                                                                                        <  1:795202/57‑1 (MQ=255)

                                                                                                 tCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAaagaag                                                                                                           <  2:769911/68‑1 (MQ=255)

                                                                                                 tCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAaagaag                                                                                                           >  1:769911/1‑68 (MQ=255)

                                                                                                     cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                         >  1:289900/1‑50 (MQ=255)

                                                                                                     cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                         <  2:289900/50‑1 (MQ=255)

                                                                                                     cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCcac                                <  1:164324/139‑1 (MQ=255)

                                                                                                     cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCcac                                >  1:848412/1‑139 (MQ=255)

                                                                                                     cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCcac                                <  1:993499/139‑1 (MQ=255)

                                                                                                        gCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAg                             >  1:786837/1‑139 (MQ=255)

                                                                                                          cGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgcg                           <  1:41551/139‑1 (MQ=255)

                                                                                                                    cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcgc                                                                                                   >  2:756078/1‑57 (MQ=255)

                                                                                                                    cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcgc                                                                                                   <  1:756078/57‑1 (MQ=255)

                                                                                                                       gatgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCg              <  1:939903/139‑1 (MQ=255)

                                                                                                                         tgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGtt            <  2:246472/139‑1 (MQ=255)

                                                                                                                           atCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGTGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCa          >  2:1048728/1‑139 (MQ=255)

                                                                                                                           atCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                 >  2:373228/1‑84 (MQ=255)

                                                                                                                           atCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                 <  1:373228/84‑1 (MQ=255)

                                                                                                                           atCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCa          >  1:858430/1‑139 (MQ=255)

                                                                                                                               tCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGTGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                 >  1:734723/1‑80 (MQ=255)

                                                                                                                               tCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGTGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                 <  2:734723/80‑1 (MQ=255)

                                                                                                                               tCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAatat      >  1:181158/1‑139 (MQ=255)

                                                                                                                                   gTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATc  >  2:278380/1‑139 (MQ=255)

ATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATC  >  NC_000913/4474257‑4474526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A