Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,428	C→T	A347A (GCG→GCA)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,428	0	C	T	97.6%	262.3 / ‑2.3	84	A347A (GCG→GCA)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (41/41); total (42/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01

CAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGTGACCGAAGCC  >  NC_000913/753294‑753562

cAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGa                                                                                                                                     >  1:809522/1‑139 (MQ=255)

  gCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATg                                                                                                                                   <  1:299478/139‑1 (MQ=255)

  gCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATg                                                                                                                                   <  1:467514/139‑1 (MQ=255)

   ccAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGt                                                                                                                                  >  2:1436768/1‑139 (MQ=255)

     aaCGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGttt                                                                                                                                <  2:291631/139‑1 (MQ=255)

        ggTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTcc                                                                                                                             >  2:523883/1‑139 (MQ=255)

         gTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCa                                                                                                                            <  1:1436768/139‑1 (MQ=255)

            cGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCt                                                                                                                         >  1:358137/1‑139 (MQ=255)

                  aTTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAg                                                                                                                   <  1:1124294/139‑1 (MQ=255)

                  aTTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAg                                                                                                                   <  1:670859/139‑1 (MQ=255)

                  aTTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAg                                                                                                                   <  1:523883/139‑1 (MQ=255)

                    tGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGcc                                                                                                                 >  1:947790/1‑139 (MQ=255)

                     gCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCa                                                                                                                <  1:928644/139‑1 (MQ=255)

                            tGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcag                                                                                                         <  2:809522/139‑1 (MQ=255)

                             gACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcagc                                                                                                        <  1:1113113/139‑1 (MQ=255)

                                  tGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTc                                                                                                   <  2:1253588/139‑1 (MQ=255)

                                    aaCATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCAt                                                                                                 >  2:218876/1‑139 (MQ=255)

                                              aCGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCt                                                                                              <  2:1074622/132‑1 (MQ=255)

                                              aCGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCt                                                                                              >  1:1074622/1‑132 (MQ=255)

                                              aCGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGcc                                                                                       >  1:113406/1‑139 (MQ=255)

                                              aCGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGc                                                                                        <  2:1561112/138‑1 (MQ=255)

                                              aCGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGc                                                                                        >  1:1561112/1‑138 (MQ=255)

                                                ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTc                                                                                            <  2:551087/132‑1 (MQ=255)

                                                ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTc                                                                                            >  1:551087/1‑132 (MQ=255)

                                                ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                                     <  2:947790/139‑1 (MQ=255)

                                                ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                                     >  2:1359501/1‑139 (MQ=255)

                                                 gAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcagc                                                                                                        >  2:1654980/1‑119 (MQ=255)

                                                 gAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcagc                                                                                                        <  1:1654980/119‑1 (MQ=255)

                                                 gAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAg                                                                                    >  1:1513981/1‑139 (MQ=255)

                                                  aaTACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCGCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGc                                                                                   <  1:1191620/139‑1 (MQ=255)

                                                         agcGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCTAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCa                                                                            >  1:1129089/3‑139 (MQ=255)

                                                          tCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGTGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTGAg                                                                           <  1:1802489/139‑1 (MQ=255)

                                                          tCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAg                                                                           <  1:547596/139‑1 (MQ=255)

                                                          tCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAg                                                                           <  1:1621736/139‑1 (MQ=255)

                                                            gCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGa                                                                                                                                     <  1:1375290/79‑1 (MQ=255)

                                                            gCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGa                                                                                                                                     >  2:1375290/1‑79 (MQ=255)

                                                                tCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTc                                                                     >  1:709083/1‑139 (MQ=255)

                                                                 cAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTcc                                                                                                                             <  2:1773873/82‑1 (MQ=255)

                                                                 cAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTcc                                                                                                                             >  1:1773873/1‑82 (MQ=255)

                                                                       gatACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAg                                                              >  1:7882/1‑139 (MQ=255)

                                                                          aCCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGtt                                                           >  2:276189/1‑139 (MQ=255)

                                                                             agagTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCa                                                        <  2:908570/139‑1 (MQ=255)

                                                                               agTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACg                                                      >  1:918244/1‑139 (MQ=255)

                                                                               agTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACg                                                      <  1:218876/139‑1 (MQ=255)

                                                                                   gAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcag                                                                                                         <  1:597452/84‑1 (MQ=255)

                                                                                   gAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcag                                                                                                         >  2:597452/1‑84 (MQ=255)

                                                                                    aaaTCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAg                                                                           <  2:999424/113‑1 (MQ=255)

                                                                                    aaaTCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAg                                                                           >  1:999424/1‑113 (MQ=255)

                                                                                      aTCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACg                                               >  1:925530/1‑139 (MQ=255)

                                                                                         gACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAg                                                                                                                   <  1:16070/68‑1 (MQ=255)

                                                                                         gACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAg                                                                                                                   >  2:16070/1‑68 (MQ=255)

                                                                                           cGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGc                                                                                   <  2:941083/98‑1 (MQ=255)

                                                                                           cGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGc                                                                                   >  1:941083/1‑98 (MQ=255)

                                                                                              tCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTCCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGgcgcg                                       <  2:1129089/139‑1 (MQ=255)

                                                                                                 ggTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGc                                                                <  1:260371/111‑1 (MQ=255)

                                                                                                 ggTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGc                                                                >  2:260371/1‑111 (MQ=255)

                                                                                                     cAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCg                                >  1:124235/1‑139 (MQ=255)

                                                                                                      aGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGt                               <  2:7882/139‑1 (MQ=255)

                                                                                                        tttCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATg                                                                                                                                   >  1:1868019/1‑37 (MQ=255)

                                                                                                        tttCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATg                                                                                                                                   <  2:1868019/37‑1 (MQ=255)

                                                                                                        tttCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACt                                                                                                              >  2:1479929/1‑58 (MQ=255)

                                                                                                        tttCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACt                                                                                                              <  1:1479929/58‑1 (MQ=255)

                                                                                                        tttCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                                     <  1:515206/83‑1 (MQ=255)

                                                                                                        tttCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                                     >  2:515206/1‑83 (MQ=255)

                                                                                                           cttctCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAg                                                                                    <  2:951553/81‑1 (MQ=255)

                                                                                                           cttctCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAg                                                                                    >  1:951553/1‑81 (MQ=255)

                                                                                                            ttctCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACg                                                      <  1:933566/110‑1 (MQ=255)

                                                                                                            ttctCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACg                                                      >  2:933566/1‑110 (MQ=255)

                                                                                                                  aTAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTaca                  >  2:562153/1‑139 (MQ=255)

                                                                                                                  aTAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTaca                  >  2:934764/1‑139 (MQ=255)

                                                                                                                        tACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGt            <  2:113406/139‑1 (MQ=255)

                                                                                                                         aCGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGAACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGTg           >  1:1351385/1‑139 (MQ=255)

                                                                                                                            ggTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGTGAcc        <  2:358137/139‑1 (MQ=255)

                                                                                                                             gTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGTGACCg       >  2:957615/1‑139 (MQ=255)

                                                                                                                                gTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACg                                                      <  1:1362705/90‑1 (MQ=255)

                                                                                                                                gTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACg                                                      >  2:1362705/1‑90 (MQ=255)

                                                                                                                                gTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGTCGCGCGGGTCGTAA‑TTTTTg                      <  2:1279152/121‑1 (MQ=255)

                                                                                                                                gTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTg                      >  1:1279152/1‑121 (MQ=255)

                                                                                                                                 ttCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                                     >  2:1309719/1‑58 (MQ=255)

                                                                                                                                 ttCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                                     <  1:1309719/58‑1 (MQ=255)

                                                                                                                                 ttCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAg                                                                                    >  2:743615/1‑59 (MQ=255)

                                                                                                                                 ttCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAg                                                                                    <  1:743615/59‑1 (MQ=255)

                                                                                                                                  tCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTTGTACACGCGGTGACCGAAGc   >  1:754578/1‑139 (MQ=255)

                                                                                                                                  tCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGTGACCGAAGcc  >  2:471384/1‑139 (MQ=255)

CAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAA‑TTTTTGTACACGCGGTGACCGAAGCC  >  NC_000913/753294‑753562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A