Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,421,933	T→C	V272A (GTC→GCC)	ydaU →	Rac prophage, conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,421,933	0	T	C	100.0%	165.2 / NA	47	V272A (GTC→GCC)	ydaU	Rac prophage, conserved protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/24); total (23/24)

CTGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAA  >  NC_000913/1421803‑1422059

cTGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTAc                                                                                                                        <  1:1869597/139‑1 (MQ=255)

  ggAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCg                                                                                                                      >  1:1799048/1‑139 (MQ=255)

  ggAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCg                                                                                                                      >  2:1686346/1‑139 (MQ=255)

   gAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCgg                                                                                                                     >  1:1006986/1‑139 (MQ=255)

                           tCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                             <  2:756984/139‑1 (MQ=255)

                              ccTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAggg                                                                                          <  2:1081425/139‑1 (MQ=255)

                                tGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAggggg                                                                                        >  1:551793/1‑139 (MQ=255)

                                     aGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACt                                                                                   <  2:1006986/139‑1 (MQ=255)

                                       gTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                             <  2:1126822/127‑1 (MQ=255)

                                       gTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                             >  1:1126822/1‑127 (MQ=255)

                                       gTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGa                                                                                 <  2:1799048/139‑1 (MQ=255)

                                       gTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGa                                                                                 >  2:1384548/1‑139 (MQ=255)

                                       gTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGa                                                                                 >  2:591079/1‑139 (MQ=255)

                                            tCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATg                                                                            >  2:1856270/1‑139 (MQ=255)

                                                   aGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                             >  2:1400975/1‑115 (MQ=255)

                                                   aGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                             <  1:1400975/115‑1 (MQ=255)

                                                     cATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                              >  2:1012666/1‑80 (MQ=255)

                                                     cATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                              <  1:1012666/80‑1 (MQ=255)

                                                     cATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                              <  1:1524002/80‑1 (MQ=255)

                                                          ccAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                              >  2:1079691/1‑75 (MQ=255)

                                                          ccAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGAc                                                              >  1:15321/1‑139 (MQ=255)

                                                           cAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACa                                                             <  2:1151572/139‑1 (MQ=255)

                                                             aTGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGAcc                                                                               >  2:1354797/1‑119 (MQ=255)

                                                             aTGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGAcc                                                                               <  1:1354797/119‑1 (MQ=255)

                                                                aaTACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGa                                                        <  2:1476903/139‑1 (MQ=255)

                                                                 aTACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGAt                                                       <  2:1160018/139‑1 (MQ=255)

                                                                  tACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATg                                                      <  2:551793/139‑1 (MQ=255)

                                                                   aCCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCg                                                         >  1:194676/1‑135 (MQ=255)

                                                                   aCCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCg                                                         <  2:194676/135‑1 (MQ=255)

                                                                    ccAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGtt                                                    >  1:642767/1‑139 (MQ=255)

                                                                        ggCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTGCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTg                                                >  1:1157625/1‑139 (MQ=255)

                                                                         gCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGa                                               >  2:1473091/1‑139 (MQ=255)

                                                                          cTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGaa                                              >  2:358443/1‑139 (MQ=255)

                                                                            tGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACg                                            <  1:1384548/139‑1 (MQ=255)

                                                                             gTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGt                                           >  2:1593411/1‑139 (MQ=255)

                                                                             gTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGt                                           <  1:1686346/139‑1 (MQ=255)

                                                                             gTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGt                                           >  1:1130530/1‑139 (MQ=255)

                                                                             gTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGt                                           >  1:104178/1‑139 (MQ=255)

                                                                                   gcCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAg                                                                                                   >  2:916660/1‑77 (MQ=255)

                                                                                   gcCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAg                                                                                                   <  1:916660/77‑1 (MQ=255)

                                                                                            tgtTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGcc                            <  1:1856270/139‑1 (MQ=255)

                                                                                             gtTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAg                                                                                                   <  1:1826594/67‑1 (MQ=255)

                                                                                             gtTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAg                                                                                                   >  2:1826594/1‑67 (MQ=255)

                                                                                                cGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTGTCCGCAGACTGGCCCCGt                        <  1:1043283/139‑1 (MQ=255)

                                                                                                cGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGt                        <  2:15321/139‑1 (MQ=255)

                                                                                                     tGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGaa                                                                          >  1:730461/1‑84 (MQ=255)

                                                                                                     tGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGaa                                                                          <  2:730461/84‑1 (MQ=255)

                                                                                                             tACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGAt                                                       >  2:267758/1‑95 (MQ=255)

                                                                                                             tACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGAt                                                       <  1:267758/95‑1 (MQ=255)

                                                                                                              aCTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACcgcg          <  1:591079/139‑1 (MQ=255)

                                                                                                                      gggTTAACACCTCCTCGTTACCAGATAGTGCGATCCCGCCAGGCTTCAGGGGGTGACTGACCATGAAAAATATTGCGTCGGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCaa  <  1:4335/138‑1 (MQ=255)

CTGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAA  >  NC_000913/1421803‑1422059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A