Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,092	A→G	V82V (GTA→GTG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,092	0	A	G	100.0%	177.7 / NA	51	V82V (GTA→GTG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/27); total (24/27)

TCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTG  >  NC_000913/2477962‑2478221

tcGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGc                                                                                                                           >  2:148603/1‑139 (MQ=255)

tcGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGc                                                                                                                           <  1:1676785/139‑1 (MQ=255)

      gAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                                     <  2:75318/139‑1 (MQ=255)

              atgGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTg                                                                                                             >  1:799318/1‑139 (MQ=255)

                    aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGTGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGc                                                                                                                           >  1:1099390/1‑119 (MQ=255)

                    aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACTATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAACAATTGGTCTGa                                                                                                       <  1:1089654/139‑1 (MQ=255)

                    aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGc                                                                                                                           <  2:1099390/119‑1 (MQ=255)

                    aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                       <  1:148603/139‑1 (MQ=255)

                        ggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCCGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACt                                                                                                   >  1:576454/1‑139 (MQ=255)

                            aCTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAAt                                                                                                               <  2:1544491/123‑1 (MQ=255)

                            aCTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAAt                                                                                                               >  1:1544491/1‑123 (MQ=255)

                                    tGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                       <  2:974191/139‑1 (MQ=255)

                                     ggTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                      <  2:799318/139‑1 (MQ=255)

                                     ggTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                      >  2:17241/1‑139 (MQ=255)

                                       tAAATGCTATTGAAAGTGGAATTGGCGGAACGCCGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTg                                                                                    <  2:576454/139‑1 (MQ=255)

                                          aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                       >  1:1708004/1‑133 (MQ=255)

                                          aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                       <  2:1708004/133‑1 (MQ=255)

                                          aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                 >  1:314528/1‑139 (MQ=255)

                                                        ggAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                        >  1:1261593/1‑134 (MQ=255)

                                                        ggAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                        <  2:1261593/134‑1 (MQ=255)

                                                          aaTTGGAGGAACGCTGGGGATGCTCGCAGCGGTTATCGGCCTTGGCACGAGACTGGGAAAAATGATGGAAGTGTCCGGGGCCGTAGCAAGAATTGGTCTGTAACTTCAACGCTGCCGCTGGCTGTCAGTTGATGTCAtt                                                                 >  1:1650208/1‑139 (MQ=255)

                                                           aTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                <  1:17241/139‑1 (MQ=255)

                                                                    aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGAGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTGCAGTTGATGTCAGTATTGTGCTgg                                                       >  2:1224651/1‑139 (MQ=255)

                                                                          gggTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACtt                                                                                                  <  2:931404/90‑1 (MQ=255)

                                                                          gggTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACtt                                                                                                  >  1:931404/1‑90 (MQ=255)

                                                                               ccTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                 >  1:1292319/1‑102 (MQ=255)

                                                                               ccTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                 <  2:1292319/102‑1 (MQ=255)

                                                                                   gCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                                        <  1:1625491/139‑1 (MQ=255)

                                                                                     aGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGc                                      >  2:466580/1‑139 (MQ=255)

                                                                                     aGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACACTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGc                                      >  1:23209/1‑139 (MQ=255)

                                                                                             tCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                              <  1:466580/139‑1 (MQ=255)

                                                                                                    tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttgtt                       >  1:1688054/1‑139 (MQ=255)

                                                                                                     ggCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTttgttg                      >  2:653746/1‑139 (MQ=255)

                                                                                                           aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                          >  2:1352801/1‑81 (MQ=255)

                                                                                                           aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                          <  1:1352801/81‑1 (MQ=255)

                                                                                                             aCTAGGAAAAATGATGGAAGTGTCCGGGACCGCAGTAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGTTGGTTGGCCTGATTTGGGGCATCGCGCTGTTTGTTGAAGTGGGc              <  2:1650208/139‑1 (MQ=255)

                                                                                                              cTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg             <  2:713717/139‑1 (MQ=255)

                                                                                                                gggAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGc                                                  >  2:1687524/1‑100 (MQ=255)

                                                                                                                gggAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGACGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGc                                                  <  1:1687524/100‑1 (MQ=255)

                                                                                                                  gAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcgtcgt         <  2:1714756/139‑1 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                      <  2:372519/58‑1 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                      >  1:372519/1‑58 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                           >  2:1182223/1‑69 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                           <  1:1182223/69‑1 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGc                                                  >  2:534261/1‑94 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGc                                                  <  1:534261/94‑1 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                >  2:1019213/1‑112 (MQ=255)

                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                <  1:1019213/112‑1 (MQ=255)

                                                                                                                         gatgGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACACTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTg  <  2:23209/139‑1 (MQ=255)

                                                                                                                            ggAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCACTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATc                                   >  2:223374/1‑103 (MQ=255)

                                                                                                                            ggAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCACTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATc                                   <  1:223374/103‑1 (MQ=255)

TCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTG  >  NC_000913/2477962‑2478221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A