Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,092	A→G	V82V (GTA→GTG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,092	0	A	G	100.0%	116.6 / NA	33	V82V (GTA→GTG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/16); total (17/16)

GTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTG  >  NC_000913/2477964‑2478215

gTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                   <  2:284653/139‑1 (MQ=255)

        gatgatGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCagaaaga                                                                                                           <  1:554444/139‑1 (MQ=255)

           gatgGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAAtt                                                                                                        >  1:1434498/1‑139 (MQ=255)

                  aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                 <  2:1434498/139‑1 (MQ=255)

                                  tGGTAAATGCTATTGAAAGTGGAATTGGCGGAGCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                 >  1:560706/1‑139 (MQ=255)

                                  tGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                 <  1:500880/139‑1 (MQ=255)

                                        aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                           >  2:1027322/1‑139 (MQ=255)

                                                 aaaGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                  <  1:416498/139‑1 (MQ=255)

                                                        aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGcc                                                                                                                    <  2:593118/82‑1 (MQ=255)

                                                        aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGcc                                                                                                                    >  1:593118/1‑82 (MQ=255)

                                                                           ttCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                    >  2:883708/1‑111 (MQ=255)

                                                                           ttCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                    <  1:883708/111‑1 (MQ=255)

                                                                             ccTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                    >  2:1324359/1‑109 (MQ=255)

                                                                             ccTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                    <  1:1324359/109‑1 (MQ=255)

                                                                              cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAAt                                                                                                         >  1:1429413/1‑71 (MQ=255)

                                                                              cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAAt                                                                                                         <  2:1429413/71‑1 (MQ=255)

                                                                              cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGAttt                                     >  2:1492693/1‑139 (MQ=255)

                                                                                cGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgt                                   >  2:884098/1‑139 (MQ=255)

                                                                                       gTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCgggg                                                                                                                      >  1:1263120/1‑49 (MQ=255)

                                                                                       gTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCgggg                                                                                                                      <  2:1263120/49‑1 (MQ=255)

                                                                                       gTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCa                            >  2:954902/1‑139 (MQ=255)

                                                                                       gTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGAGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCa                            <  1:1408072/139‑1 (MQ=255)

                                                                                        ttATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCAc                           <  2:100382/139‑1 (MQ=255)

                                                                                        ttATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCAc                           >  1:100382/1‑139 (MQ=255)

                                                                                         tATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGc                                                                                                                     <  2:161067/48‑1 (MQ=255)

                                                                                         tATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGc                                                                                                                     >  1:161067/1‑48 (MQ=255)

                                                                                         tATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                          <  2:560706/139‑1 (MQ=255)

                                                                                          aTCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGc                         >  2:1364021/1‑139 (MQ=255)

                                                                                                  tGGCCCGATACTGGGAAAAATGATGGAAGTGTACGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttgtt                 >  2:78447/1‑139 (MQ=255)

                                                                                                         aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCAGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCTTCACGCTGTTTGTTGAAGTgg          >  2:892214/1‑139 (MQ=255)

                                                                                                                 aaaaaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTg  <  1:1492693/139‑1 (MQ=255)

                                                                                                                    aaTGATGGAAGTGTCCGGGGCCGCCGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                          <  1:975946/112‑1 (MQ=255)

                                                                                                                    aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                          >  2:975946/1‑112 (MQ=255)

GTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTG  >  NC_000913/2477964‑2478215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A